LOCUS       V008820                 6167 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008820
VERSION     V008820
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6167)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6167)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             72..950
                     /gene="CASP6"
                     /label="Caspase-6"
                     /note="Caspase-6 from Homo sapiens. Accession#: P55212"
     misc_feature    954..1403
                     /label="3UTR"
                     /note="3UTR"
     polyA_signal    1524..1579
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1940..1956)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1964..1980)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1988..2018)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2032..2053)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2111..2307
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2313..2447
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2685..3273)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3447..4304)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4305..4409)
                     /label="AmpR promoter"
     enhancer        4440..4819
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4440..4817
                     /label="hCMV"
                     /note="hCMV"
     promoter        4821..5098
                     /label="chicken beta-actin promoter"
     intron          5099..6116
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 gtcgagctgc aatgagctcg gcctcggggc tccgcagggg gcacccggca ggtggggaag
      121 aaaacatgac agaaacagat gccttctata aaagagaaat gtttgatccg gcagaaaagt
      181 acaaaatgga ccacaggagg agaggaattg ctttaatctt caatcatgag aggttctttt
      241 ggcacttaac actgccagaa aggcggcgca cctgcgcaga tagagacaat cttacccgca
      301 ggttttcaga tctaggattt gaagtgaaat gctttaatga tcttaaagca gaagaactac
      361 tgctcaaaat tcatgaggtg tcaactgtta gccacgcaga tgccgattgc tttgtgtgtg
      421 tcttcctgag ccatggcgaa ggcaatcaca tttatgcata tgatgctaaa atcgaaattc
      481 agacattaac tggcttgttc aaaggagaca agtgtcacag cctggttgga aaacccaaga
      541 tatttatcat ccaggcatgt cggggaaacc agcacgatgt gccagtcatt cctttggatg
      601 tagtagataa tcagacagag aagttggaca ccaacataac tgaggtggat gcagcctccg
      661 tttacacgct gcctgctgga gctgacttcc tcatgtgtta ctctgttgca gaaggatatt
      721 attctcaccg ggaaactgtg aacggctcat ggtacattca agatttgtgt gagatgttgg
      781 gaaaatatgg ctcctcctta gagttcacag aactcctcac actggtgaac aggaaagttt
      841 ctcagcgccg agtggacttt tgcaaagacc caagtgcaat tggaaagaag caggttccct
      901 gttttgcctc aatgctaact aaaaagctgc atttctttcc aaaatctaat taattaatag
      961 aggctatcta atttcacact ctgtattgaa aatggctttc tcagccaggc gtggttactc
     1021 acacctgtaa tcccagcact ttgggagtcc aaggtgggcg gatcacctga ggtcgggagt
     1081 tcgagaccag cctgaccaac atggcagaag ccccgcctct actaaaaatg caaaaaaaaa
     1141 tttagctagg catggcggcg catgcctgca atcccagcta cttggaaggc tgaggcagga
     1201 gaatcacttg aacccaggag gtggaggctg cggtgagccg agcattgcgc cattgcactc
     1261 cagcctgggc aacgagtgaa actccgtctc aaaaaaaaag aaaatgtctt tctcttcctt
     1321 ttatataaat atcgttaggg tgaagcatta tggtctaatg attcaaatgt tttaaagttt
     1381 aatgcctagc agagaactgc cttaaaaaaa aaaaaaaaaa aactcgacca atgccctggc
     1441 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     1501 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     1561 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     1621 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     1681 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     1741 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     1801 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     1861 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     1921 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1981 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag
     2041 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2101 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2281 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     2461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2521 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2581 ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc
     2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
     4441 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4501 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4561 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4621 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4681 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4741 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4801 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     4861 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     4921 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg
     4981 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     5041 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     5101 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     5161 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     5221 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     5281 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     5341 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     5401 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     5461 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     5521 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     5581 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     5641 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     5701 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg
     5761 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     5821 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     5881 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     5941 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     6001 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     6061 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     6121 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa
//
