LOCUS       40924_8091        5340 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-deltaPTB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             8..628
                     /codon_start=1
                     /note="unnamed protein product; deltaPTB"
                     /protein_id="SJL87092.1"
                     /translation="MVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFI
                     EMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQ
                     SGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFT
                     KNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKL"
     misc_feature    20..274
                     /label=RRM1
                     /note="RRM1"
     misc_feature    395..625
                     /label=RRM2
                     /note="RRM2"
     polyA_signal    697..752
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1113..1129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1137..1153)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1161..1191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1205..1226)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1284..1480
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1486..1620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1858..2446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2620..3477)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3478..3582)
                     /label=AmpR promoter
     enhancer        3613..3992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3613..3990
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        3994..4271
                     /label=chicken beta-actin promoter
     intron          4272..5289
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg gtcccctcta gagtgatcca catccggaag ctccccatcg acgtcacgga
       61 gggggaagtc atctccctgg ggctgccctt tgggaaggtc accaacctcc tgatgctgaa
      121 ggggaaaaac caggccttca tcgagatgaa cacggaggag gctgccaaca ccatggtgaa
      181 ctactacacc tcggtgaccc ctgtgctgcg cggccagccc atctacatcc agttctccaa
      241 ccacaaggag ctgaagaccg acagctctcc caaccaggcg cgggcccagg cggccctgca
      301 ggcggtgaac tcggtccagt cggggaacct ggccttggct gcctcggcgg cggccgtgga
      361 cgcagggatg gcgatggccg ggcagagccc cgtgctcagg atcatcgtgg agaacctctt
      421 ctaccctgtg accctggatg tgctgcacca gattttctcc aagttcggca cagtgttgaa
      481 gatcatcacc ttcaccaaga acaaccagtt ccaggccctg ctgcagtatg cggaccccgt
      541 gagcgcccag cacgccaagc tgtcgctgga cgggcagaac atctacaacg cctgctgcac
      601 gctgcgcatc gacttttcca agctctagag atctttttcc ctctgccaaa aattatgggg
      661 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
      721 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
      781 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
      841 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
      901 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
      961 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     1021 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     1081 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1141 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
     1201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1261 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     1321 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1441 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     1621 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1681 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
     1801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3601 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     3661 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     3721 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     3781 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     3841 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     3901 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     3961 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
     4021 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
     4081 tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg
     4141 gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc
     4201 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     4261 cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct
     4321 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     4381 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     4441 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     4501 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     4561 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     4621 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     4681 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     4741 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     4801 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
     4861 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc
     4921 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
     4981 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
     5041 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
     5101 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
     5161 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     5221 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     5281 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//
