LOCUS       40924_8516        6757 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-TS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6757)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             73..2004
                     /codon_start=1
                     /note="unnamed protein product; mature TS"
                     /protein_id="SJL88093.1"
                     /translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNVD
                     GVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVIVK
                     GNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVSLKK
                     FFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKTWKFG
                     KGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVWGPSPK
                     SDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQVSIGDE
                     NSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDSHLSSICT
                     PADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAERVRNGLKFA
                     GVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLDSSGGKKLLG
                     LSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGELLEGSGQTVVP
                     DGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFLSQDLIGTEAHM
                     DSSSDTSA"
     polyA_signal    2114..2169
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2530..2546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2554..2570)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2578..2608)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2622..2643)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2701..2897
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    2903..3037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3275..3863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4037..4894)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4895..4999)
                     /label=AmpR promoter
     enhancer        5030..5409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5030..5407
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5411..5688
                     /label=chicken beta-actin promoter
     intron          5689..6706
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgagg aattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
       61 ggccatggcc atatgctggc acccggatcg agccgagttg agctgtttaa gcgtaagaat
      121 tcgacggtgc cgtttgaaga caaggccggc aaagtcaccg agcgggttgt ccactcgttc
      181 cgcctccccg cccttgttaa tgtggacggg gtgatggttg ccatcgcgga cgctcgctac
      241 gacacatcca atgacaactc cctcattgat acggtggcga agtacagcgt ggacgatggg
      301 gagacgtggg agacccaaat tgccatcaag aacagccgtg tatcgtctgt ttctcgtgtg
      361 gtggatccca ccgtgattgt gaagggcaac aagctttacg tcctggttgg aagctactat
      421 agttcgagaa gctactggtc gtcgcatggt gatgcgagag actgggatat tctgcttgcc
      481 gttggtgagg tcacgaagtc cattgcgggc ggcaagataa ctgcgagtat caaatggggg
      541 agccccgtgt cactgaagaa gttttttccg gcagaaatgg aaggcatgca cacaaatcaa
      601 tttcttggcg gcgcgggtgt tgccattgtg gcgtccaacg ggaatcttgt gtaccctgtg
      661 caggttacga acaaaaggaa gcaagttttc tccaagatct tctactcgga agatgatggg
      721 aagacgtgga agtttgggaa gggtaggagc gattttggct gctctgaacc tgtggccctt
      781 gagtgggagg ggaagctcat cataaacacc cgagttgact ggaaacgccg tctggtgtac
      841 gagtccagtg acatggggaa tacgtgggtg gaggctgtcg gcacgctctc gcgtgtgtgg
      901 ggcccctcac caaaatcgga ccatcccggc agtcagagca gcttcactgc cgtgaccatc
      961 gaaggaatgc gtgtgatgct cttcacacac ccgctgaatt ttaagggaag gtggctgcgc
     1021 gaccgactga acctctggct gacggataac cagcgcattt ataacgttgg gcaagtatcc
     1081 attggtgatg aaaattccgc ctacagctcc gtcctgtaca aggatgataa gctgtactgt
     1141 ttgcatgaga tcaacacgga cgaggtgtac agccttgttt ttgcacgcct ggttggcgag
     1201 ctacggatca ttaaatcagt gctgcgatcc tggaagaatt gggacagcca cctgtccagc
     1261 atttgcaccc ctgctgatcc agccgcttcg tcgtcagaga gtggttgtgg tcccgctgtc
     1321 accacggttg gtcttgttgg ctttttgtcc ggcaatgcct cccaaaacgt atgggaggat
     1381 gcgtaccgct gcgtcaacgc aagcacggca aatgcggaga gggttcggaa cggtttgaag
     1441 tttgcggggg ttggcggagg ggcgctttgg ccggtgagcc agcaggggca gaatcagcgg
     1501 tatcgttttg caaaccacgc gttcacgctg gtggcgtcgg tgacgattca cgaggctccg
     1561 agggccgcga gtcctttgct gggtgcgagc ctggactctt ctggtggcaa aaaactcctg
     1621 gggctctcgt acgacgagaa gcaccagtgg cagccaatat acggatcaac gccggtgacg
     1681 ccgacgggat cgtgggagac gggtaagagg taccacgtgg ttcttacggt ggcgaataaa
     1741 attggctccg tgtacattga tggagaactt ctggagggtt caggacagac cgttgtgcca
     1801 gacgggagga cgcctgacat ctcccacttc tacgttggcg ggtatggaag gagtgatatg
     1861 ccaaccataa gccacgtgac ggtgaataat gttcttcttt acaaccgaca gctgaatacc
     1921 gaggagatca ggaccttgtt cttgagccag gacctgattg gcacggaagc acacatggac
     1981 agcagcagcg acacgagtgc ctgatcaggc ctaggcccca atgccctggc tcacaaatac
     2041 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     2101 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     2161 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     2221 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     2281 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     2341 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     2401 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     2461 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     2521 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2581 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
     2641 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     2701 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2821 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2881 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     2941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3001 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     3061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     3181 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta
     5041 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5101 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5161 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5221 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5281 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5341 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5401 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     5461 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     5521 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     5581 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     5641 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg
     5701 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     5761 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     5821 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     5881 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     5941 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     6001 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     6061 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     6121 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     6181 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     6241 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     6301 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
     6361 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     6421 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     6481 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
     6541 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
     6601 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
     6661 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     6721 gctggttatt gtgctgtctc atcattttgg caaagaa
//
