LOCUS       40924_8511        6560 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-TAX1BP1-S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6560)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6560)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..1710
                     /codon_start=1
                     /note="unnamed protein product; hTAX1BP1-S"
                     /protein_id="SJL88313.1"
                     /translation="MERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKKRFSDATS
                     KAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKELYKVHLK
                     NTEIENTKLMSEVQTLKNLDGNKESVITHFKEEIGRLQLCLAEKENLQRTFLLTTSSKE
                     DTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENEKVKKQLA
                     DAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQRLQKQINK
                     LSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFDIVTKGQV
                     CEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENVKLELAEV
                     QDNYKELKRSLENPAERKMEDGADGAFYPDEIQRPPVRVPSWGLEDNVVCSQPARNFSR
                     PDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCELMFPPNYDQ
                     SKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFDQNVLNFD"
     polyA_signal    1919..1974
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2335..2351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2359..2375)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2383..2413)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2427..2448)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2506..2702
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    2708..2842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3080..3668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3842..4699)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4700..4804)
                     /label=AmpR promoter
     enhancer        4835..5214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4835..5212
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5216..5493
                     /label=chicken beta-actin promoter
     intron          5494..6511
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 aattcggcac gagggagaat ggaaagagaa cttaaccatg agaaagaaag atgtgaccaa
       61 ctgcaagcag aacaaaaggg tcttactgaa gtaacacaaa gcttaaaaat ggaaaatgaa
      121 gagtttaaga agaggttcag tgatgctaca tccaaagccc atcagcttga ggaagatatt
      181 gtgtcagtaa cacataaagc aattgaaaaa gaaaccgaat tagacagttt aaaggacaaa
      241 ctcaagaagg cacaacatga aagagaacaa cttgaatgtc agttgaagac agagaaggat
      301 gaaaaggaac tttataaggt acatttgaag aatacagaaa tagaaaatac caagcttatg
      361 tcagaggtcc agactttaaa aaatttagat gggaacaaag aaagcgtgat tactcatttc
      421 aaagaagaga ttggcaggct gcagttatgt ttggctgaaa aggaaaatct gcaaagaact
      481 ttcctgctta caacctcaag taaagaagat acttgttttt taaaggagca acttcgtaaa
      541 gcagaggaac aggttcaggc aactcggcaa gaagttgtct ttctggctaa agaactcagt
      601 gatgctgtca acgtacgaga cagaacgatg gcagacctgc atactgcacg cttggaaaac
      661 gagaaagtga aaaagcagtt agctgatgca gtggcagaac ttaaactaaa tgctatgaaa
      721 aaagatcagg acaagactga tacactggaa cacgaactaa gaagagaagt tgaagatctg
      781 aaactccgtc ttcagatggc tgcagaccat tataaagaaa aatttaagga atgccaaagg
      841 ctccaaaaac aaataaacaa actttcagat caatcagcta ataataataa tgtcttcaca
      901 aagaaaacgg ggaatcagca gaaagtgaat gatgcttcag taaacacaga cccagccact
      961 tctgcctcta ctgtagatgt aaagccatca ccttctgcag cagaggcaga ttttgacata
     1021 gtaacaaagg ggcaagtctg tgaaatgacc aaagaaattg ctgacaaaac agaaaagtat
     1081 aataaatgta aacaactctt gcaggatgag aaagcaaaat gcaataaata tgctgatgaa
     1141 cttgcaaaaa tggagctgaa atggaaagaa caagtgaaaa ttgctgaaaa tgtaaaactt
     1201 gaactagctg aagtacagga caattataaa gaacttaaaa ggagtctaga aaatccagca
     1261 gaaaggaaaa tggaagatgg agcagatggt gctttttacc cagatgaaat acaaaggcca
     1321 cctgtcagag tcccctcttg gggactggaa gacaatgttg tctgcagcca gcctgctcga
     1381 aactttagtc ggcctgatgg cttagaggac tctgaggata gcaaagaaga tgagaatgtg
     1441 cctactgctc ctgatcctcc aagtcaacat ttacgtgggc atgggacagg cttttgcttt
     1501 gattccagct ttgatgttca caagaagtgt cccctctgtg agttaatgtt tcctcctaac
     1561 tatgatcaga gcaaatttga agaacatgtt gaaagtcact ggaaggtgtg cccgatgtgc
     1621 agcgagcagt tccctcctga ctatgaccag caggtgtttg aaaggcatgt gcagacccat
     1681 tttgatcaga atgttctaaa ttttgactag ttacttttta ttatgagtta atatagttta
     1741 gcagtaaaaa aaaaaaaaaa aaaactcgag gaattcactc ctcaggtgca ggctgcctat
     1801 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     1861 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     1921 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     1981 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     2041 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     2101 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     2161 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     2221 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     2281 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     2341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2401 cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2461 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     2521 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2581 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2641 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2701 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2821 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     2881 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2941 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3781 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3841 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3901 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3961 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4021 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4081 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4141 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4201 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4261 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4321 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4381 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4441 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4501 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4561 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4621 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4681 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4741 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4801 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
     4861 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     4921 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     4981 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     5041 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     5101 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     5161 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
     5221 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     5281 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc
     5341 cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg
     5401 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     5461 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc
     5521 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
     5581 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
     5641 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
     5701 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     5761 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     5821 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc
     5881 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg
     5941 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
     6001 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
     6061 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
     6121 ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     6181 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     6241 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     6301 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     6361 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     6421 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     6481 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     6541 gtctcatcat tttggcaaag
//
