LOCUS       40924_8506        7189 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-TAX1BP1-L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             92..2335
                     /codon_start=1
                     /note="unnamed protein product; hTAX1BP1-L"
                     /protein_id="SJL88026.1"
                     /translation="MTSFQEVPLQTSNFAHVIFQNVAKSYLPNAHLECHYTLTPYIHPH
                     PKDWVGIFKVGWSTARDYYTFLWSPMPEHYVEGSTVNCVLAFQGYYLPNDDGEFYQFCY
                     VTHKGEIRGASTPFQFRASSPVEELLTMEDEGNSDMLVVTTKAGLLELKIEKTMKEKEE
                     LLKLIAVLEKETAQLREQVGRMERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKK
                     RFSDATSKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKE
                     LYKVHLKNTEIENTKLMSEVQTLKNIDGNKESVITHFKEEIGRLQLCLAEKENLQRTFL
                     LTTSSKEDTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENE
                     KVKKQLADAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQR
                     LQKQINKLSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFD
                     IVTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENV
                     KLELAEVQDNYKELKRSLENPAERKMEDGADGAFYPDEIQRPPVRVPSWGLEDNVVCSQ
                     PARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCELM
                     FPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFDQNVLNFD"
     polyA_signal    2544..2599
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2960..2976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2984..3000)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3008..3038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3052..3073)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3131..3327
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    3333..3467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3705..4293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4467..5324)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5325..5429)
                     /label=AmpR promoter
     enhancer        5464..5843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5464..5841
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5845..6122
                     /label=chicken beta-actin promoter
     intron          6123..7140
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 aattcnnngg ttcggcggct gatggcggat caggatcgga agcctgcgta actttctccc
       61 ttgatccggg agtctttcca ctggattcac aatgacatcc tttcaagaag tcccattgca
      121 gacttccaac tttgcccatg tcatctttca aaatgtggcc aagagttacc ttcctaatgc
      181 acacctggaa tgtcattaca ccttaactcc atatattcat ccacatccaa aagattgggt
      241 tggtatattc aaggttggat ggagtactgc tcgtgattat tacacgtttt tatggtcccc
      301 tatgcctgaa cattatgtgg aaggatcaac agtcaattgt gtactagcat tccaaggata
      361 ttaccttcca aatgatgatg gagaatttta tcagttctgt tacgttaccc ataagggtga
      421 aattcgtgga gcaagtacac ctttccagtt tcgagcttct tctccagttg aagagctgct
      481 tactatggaa gatgaaggaa attctgacat gttagtggtg accacaaaag caggccttct
      541 tgagttgaaa attgagaaaa ccatgaaaga aaaagaagaa ctgttaaagt taattgccgt
      601 tctggaaaaa gaaacagcac aacttcgaga acaagttggg agaatggaaa gagaacttaa
      661 ccatgagaaa gaaagatgtg accaactgca agcagaacaa aagggtctta ctgaagtaac
      721 acaaagctta aaaatggaaa atgaagagtt taagaagagg ttcagtgatg ctacatccaa
      781 agcccatcag cttgaggaag atattgtgtc agtaacacat aaagcaattg aaaaagaaac
      841 cgaattagac agtttaaagg acaaactcaa gaaggcacaa catgaaagag aacaacttga
      901 atgtcagttg aagacagaga aggatgaaaa ggaactttat aaggtacatt tgaagaatac
      961 agaaatagaa aataccaagc ttatgtcaga ggtccagact ttaaaaaata tagatgggaa
     1021 caaagaaagc gtgattactc atttcaaaga agagattggc aggctgcagt tatgtttggc
     1081 tgaaaaggaa aatctgcaaa gaactttcct gcttacaacc tcaagtaaag aagatacttg
     1141 ttttttaaag gagcaacttc gtaaagcaga ggaacaggtt caggcaactc ggcaagaagt
     1201 tgtctttctg gctaaagaac tcagtgatgc tgtcaacgta cgagacagaa cgatggcaga
     1261 cctgcatact gcacgcttgg aaaacgagaa agtgaaaaag cagttagctg atgcagtggc
     1321 agaacttaaa ctaaatgcta tgaaaaaaga tcaggacaag actgatacac tggaacacga
     1381 actaagaaga gaagttgaag atctgaaact ccgtcttcag atggctgcag accattataa
     1441 agaaaaattt aaggaatgcc aaaggctcca aaaacaaata aacaaacttt cagatcaatc
     1501 agctaataat aataatgtct tcacaaagaa aacggggaat cagcagaaag tgaatgatgc
     1561 ttcagtaaac acagacccag ccacttctgc ctctactgta gatgtaaagc catcaccttc
     1621 tgcagcagag gcagattttg acatagtaac aaaggggcaa gtctgtgaaa tgaccaaaga
     1681 aattgctgac aaaacagaaa agtataataa atgtaaacaa ctcttgcagg atgagaaagc
     1741 aaaatgcaat aaatatgctg atgaacttgc aaaaatggag ctgaaatgga aagaacaagt
     1801 gaaaattgct gaaaatgtaa aacttgaact agctgaagta caggacaatt ataaagaact
     1861 taaaaggagt ctagaaaatc cagcagaaag gaaaatggaa gatggagcag atggtgcttt
     1921 ttacccagat gaaatacaaa ggccacctgt cagagtcccc tcttggggac tggaagacaa
     1981 tgttgtctgc agccagcctg ctcgaaactt tagtcggcct gatggcttag aggactctga
     2041 ggatagcaaa gaagatgaga atgtgcctac tgctcctgat cctccaagtc aacatttacg
     2101 tgggcatggg acaggctttt gctttgattc cagctttgat gttcacaaga agtgtcccct
     2161 ctgtgagtta atgtttcctc ctaactatga tcagagcaaa tttgaagaac atgttgaaag
     2221 tcactggaag gtgtgcccga tgtgcagcga gcagttccct cctgactatg accagcaggt
     2281 gtttgaaagg catgtgcaga cccattttga tcagaatgtt ctaaattttg actagttact
     2341 ttttattatg agttaatata gtttagcagt aaaaaaaaaa aaaaaaaaac tcgaggaatt
     2401 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     2461 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     2521 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2581 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     2641 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     2701 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     2761 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     2821 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     2881 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     2941 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag
     3061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3121 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3181 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3241 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3301 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     3361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     3481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3601 ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc
     3661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
     5461 atcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     5521 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5581 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     5641 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     5701 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     5761 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     5821 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     5881 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     5941 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
     6001 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     6061 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     6121 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
     6181 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     6241 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     6301 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     6361 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     6421 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     6481 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     6541 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     6601 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     6661 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     6721 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     6781 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     6841 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     6901 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     6961 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     7021 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     7081 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     7141 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaag
//
