LOCUS       Exported                7421 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Exported.
ACCESSION   V008802
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Paterson RG, Russell CJ, Lamb RA.
  TITLE     Fusion protein of the paramyxovirus SV5: destabilizing and 
            stabilizing mutants of fusion activation
  JOURNAL   Virology 270 (1), 17-30 (2000)
  PUBMED    10772976
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS.
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic 
            tracing and high-level gene expression in neurons
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7421)
  AUTHORS   Wickersham IR.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes 
            Medical Institute and Massachusetts Institute of Technology, 43 
            Vassar St., Cambridge, MA 02139, USA
REFERENCE   4  (bases 1 to 7421)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7421)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology"; 
            date: "2000"; volume: "270"; issue: "1"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2009) Brain and Cognitive Sciences, Howard Hughes Medical 
            Institute and Massachusetts Institute of Technology, 43 Vassar St., 
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..642
                     /label=chicken beta-actin promoter
     intron          644..1659
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1750..4398
                     /codon_start=1
                     /label=T7 RNA polymerase
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE
                     MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ
                     FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK
                     KNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIE
                     STGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGI
                     TGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANV
                     ITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLE
                     FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYY
                     WLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
                     YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAK
                     KVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM
                     TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE
                     AMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG
                     QFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD
                     SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN
                     LRDILESDFAFA"
     polyA_signal    4490..4545
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4906..4922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4930..4946)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4954..4984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4999..5020)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5078..5274
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    5280..5414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5653..6241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6415..7272)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7273..7377)
                     /label=AmpR promoter
     promoter        7405..7421
                     /label=CAG
                     /note="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc ccctccccac
      421 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      481 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      541 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      601 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      661 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      721 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      781 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      841 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      901 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      961 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     1021 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     1081 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     1141 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     1201 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     1261 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     1321 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     1381 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     1441 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1501 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1561 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1621 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1681 attgtgctgt ctcatcattt tggcaaagaa ttcgagctca tcgatgcatg gtacccgggc
     1741 atgctcgaga tgaacacgat taacatcgct aagaacgact tctctgacat cgaactggct
     1801 gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg
     1861 gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt
     1921 caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta
     1981 ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc
     2041 aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc
     2101 accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta
     2161 gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt
     2221 gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc
     2281 tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt
     2341 ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc
     2401 gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta
     2461 ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt
     2521 gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg
     2581 tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg
     2641 cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac
     2701 aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc
     2761 gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgaacgt
     2821 gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg
     2881 aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc
     2941 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct
     3001 tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac
     3061 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac
     3121 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag
     3181 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg
     3241 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag
     3301 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac
     3361 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc
     3421 gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa
     3481 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg
     3541 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct
     3601 ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg
     3661 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca
     3721 gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg
     3781 gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac
     3841 tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag
     3901 attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag
     3961 gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta
     4021 cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt
     4081 atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg
     4141 gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt
     4201 ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag
     4261 tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa
     4321 ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag
     4381 tcggacttcg cgttcgcgta acgccaaatc aatacgactc cggatctttt tccctctgcc
     4441 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     4501 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     4561 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     4621 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     4681 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     4741 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     4801 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     4861 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     4921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5041 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     5101 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5161 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5221 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     5281 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5341 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5401 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6061 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7381 atttccccga aaagtgccac ctgggtcgac attgattatt g
//
