LOCUS       V043037                 7133 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043037
VERSION     V043037
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1738..1764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1771..4131
                     /label="STAT5B(NM_012448)"
                     /note="STAT5B(NM_012448)"
                     /gene="STAT5B"
     polyA_signal    4220..4275
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(4636..4652)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4660..4676
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4684..4714)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4729..4750
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        4809..5004
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    5010..5144
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5382..5970)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6141..7001)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7002..7106)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac
     1741 ccatacgatg ttccagatta cgctgaattc atggctgtgt ggatacaagc tcagcagctc
     1801 caaggagaag cccttcatca gatgcaagcg ttatatggcc agcattttcc cattgaggtg
     1861 cggcattatt tatcccagtg gattgaaagc caagcatggg actcagtaga tcttgataat
     1921 ccacaggaga acattaaggc cacccagctc ctggagggcc tggtgcagga gctgcagaag
     1981 aaggcagagc accaggtggg ggaagatggg tttttactga agatcaagct ggggcactat
     2041 gccacacagc tccagaacac gtatgaccgc tgccccatgg agctggtccg ctgcatccgc
     2101 catatattgt acaatgaaca gaggttggtc cgagaagcca acaatggtag ctctccagct
     2161 ggaagccttg ctgatgccat gtcccagaaa cacctccaga tcaaccagac gtttgaggag
     2221 ctgcgactgg tcacgcagga cacagagaat gagttaaaaa agctgcagca gactcaggag
     2281 tacttcatca tccagtacca ggagagcctg aggatccaag ctcagtttgg cccgctggcc
     2341 cagctgagcc cccaggagcg tctgagccgg gagacggccc tccagcagaa gcaggtgtct
     2401 ctggaggcct ggttgcagcg tgaggcacag acactgcagc agtaccgcgt ggagctggcc
     2461 gagaagcacc agaagaccct gcagctgctg cggaagcagc agaccatcat cctggatgac
     2521 gagctgatcc agtggaagcg gcggcagcag ctggccggga acggcgggcc ccccgagggc
     2581 agcctggacg tgctacagtc ctggtgtgag aagttggccg agatcatctg gcagaaccgg
     2641 cagcagatcc gcagggctga gcacctctgc cagcagctgc ccatccccgg cccagtggag
     2701 gagatgctgg ccgaggtcaa cgccaccatc acggacatta tctcagccct ggtgaccagc
     2761 acgttcatca ttgagaagca gcctcctcag gtcctgaaga cccagaccaa gtttgcagcc
     2821 actgtgcgcc tgctggtggg cgggaagctg aacgtgcaca tgaacccccc ccaggtgaag
     2881 gccaccatca tcagtgagca gcaggccaag tctctgctca agaacgagaa cacccgcaat
     2941 gattacagtg gcgagatctt gaacaactgc tgcgtcatgg agtaccacca agccacaggc
     3001 acccttagtg cccacttcag gaatatgtcc ctgaaacgaa ttaagaggtc agaccgtcgt
     3061 ggggcagagt cggtgacaga agaaaaattt acaatcctgt ttgaatccca gttcagtgtt
     3121 ggtggaaatg agctggtttt tcaagtcaag accctgtccc tgccagtggt ggtgatcgtt
     3181 catggcagcc aggacaacaa tgcgacggcc actgttctct gggacaatgc ttttgcagag
     3241 cctggcaggg tgccatttgc cgtgcctgac aaagtgctgt ggccacagct gtgtgaggcg
     3301 ctcaacatga aattcaaggc cgaagtgcag agcaaccggg gcctgaccaa ggagaacctc
     3361 gtgttcctgg cgcagaaact gttcaacaac agcagcagcc acctggagga ctacagtggc
     3421 ctgtctgtgt cctggtccca gttcaacagg gagaatttac caggacggaa ttacactttc
     3481 tggcaatggt ttgacggtgt gatggaagtg ttaaaaaaac atctcaagcc tcattggaat
     3541 gatggggcca ttttggggtt tgtaaacaag caacaggccc atgacctact cattaacaag
     3601 ccagatggga ccttcctcct gagattcagt gactcagaaa ttggcggcat caccattgct
     3661 tggaagtttg attctcagga aagaatgttt tggaatctga tgccttttac caccagagac
     3721 ttctccattc ggtccctagc cgaccgcttg ggagacttga attaccttat ctacgtgttt
     3781 cctgatcggc caaaagatga agtatactcc aaatactaca caccagttcc ctgcgagtct
     3841 gctactgcta aagctgttga tggatacgtg aagccacaga tcaagcaagt ggtccctgag
     3901 tttgtgaacg catctgcaga tgccgggggc ggcagcgcca cgtacatgga ccaggccccc
     3961 tccccagctg tgtgtcccca ggctcactat aacatgtacc cacagaaccc tgactcagtc
     4021 cttgacaccg atggggactt cgatctggag gacacaatgg acgtagcgcg gcgtgtggag
     4081 gagctcctgg gccggccaat ggacagtcag tggatcccgc acgcacaatc gtgaagatct
     4141 taattaatta aggatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     4201 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     4261 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     4321 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     4381 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     4441 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     4501 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     4561 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     4621 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4681 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4741 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4801 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4861 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4921 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4981 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     5041 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5101 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     5161 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5281 aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag
     5341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6421 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7021 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7081 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgg
//
