LOCUS       V031519                 5127 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031519
VERSION     V031519
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     regulatory      1..10
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             10..39
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             64..387
                     /label="RBX1(NM_014248)"
                     /note="RBX1(NM_014248)"
                     /gene="RBX1"
     polyA_signal    487..542
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(903..919)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    927..943
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(951..981)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    995..1016
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        1075..1270
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    1276..1410
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(1648..2236)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(2407..3267)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3268..3372)
                     /gene="bla"
                     /label="AmpR promoter"
     enhancer        3403..3782
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        3784..4061
                     /label="chicken β-actin promoter"
     intron          4062..5079
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
ORIGIN
        1 gccaccatgg aacaaaaact catctcagaa gaggatctgg aattcgagct catcgatggt
       61 accatggcgg cagcgatgga tgtggatacc ccgagcggca ccaacagcgg cgcgggcaag
      121 aagcgctttg aagtgaaaaa gtggaatgca gtagccctct gggcctggga tattgtggtt
      181 gataactgtg ccatctgcag gaaccacatt atggatcttt gcatagaatg tcaagctaac
      241 caggcgtccg ctacttcaga agagtgtact gtcgcatggg gagtctgtaa ccatgctttt
      301 cacttccact gcatctctcg ctggctcaaa acacgacagg tgtgtccatt ggacaacaga
      361 gagtgggaat tccaaaagta tgggcactag ctcgagctag cagatcttaa ttaattaagg
      421 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
      481 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
      541 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
      601 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
      661 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
      721 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
      781 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
      841 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
      901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      961 gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc acattaattg
     1021 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     1081 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1141 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1201 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1261 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1321 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1381 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
     1441 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1561 cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2521 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     3301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3361 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgacattga ttattgacta
     3421 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     3481 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3541 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3601 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3661 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3721 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3781 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
     3841 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     3901 gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggaaag
     3961 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     4021 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc
     4081 ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     4141 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     4201 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     4261 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     4321 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     4381 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     4441 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     4501 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     4561 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     4621 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     4681 agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
     4741 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
     4801 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     4861 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     4921 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     4981 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     5041 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     5101 attgtgctgt ctcatcattt tggcaaa
//
