LOCUS       62056_4570        4809 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             10..39
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     polyA_signal    169..224
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(585..601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(609..625)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(633..663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(677..698)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        756..952
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    958..1092
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1330..1918)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2092..2949)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2950..3054)
                     /label=AmpR promoter
     intron          3744..4761
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 gccaccatgg aacaaaaact catctcagaa gaggatctgg aattcgagct catcgatggt
       61 acccgggcat gcctcgagct agcagatctt aattaattaa ggatcttttt ccctctgcca
      121 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
      181 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
      241 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
      301 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
      361 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
      421 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
      481 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
      541 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
      601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      661 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      721 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
      781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
      901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
      961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1081 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1141 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc
     1261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     2221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     2281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     2881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     2941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3061 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
     3121 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     3181 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     3241 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     3301 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     3361 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     3421 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     3481 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     3541 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg
     3601 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc
     3661 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     3721 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct
     3781 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     3841 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     3901 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     3961 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     4021 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     4081 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     4141 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     4201 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     4261 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     4321 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg
     4381 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     4441 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     4501 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     4561 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     4621 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     4681 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     4741 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     4801 tttggcaaa
//
