LOCUS       V038530                 7366 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038530
VERSION     V038530
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     regulatory      1729..1738
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             1738..1761
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             1768..4353
                     /label="MARCHF6(NM_001270660)"
                     /note="MARCHF6(NM_001270660)"
                     /gene="MARCHF6"
     polyA_signal    4453..4508
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(4869..4885)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4893..4909
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4917..4947)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4962..4983
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        5042..5237
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    5243..5377
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5615..6203)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6374..7234)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7235..7339)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatggat
     1741 tacaaggatg acgacgataa ggaattcatg gacaccgcgg aggaagacat atgtagagtg
     1801 tgtcggtcag aaggaacacc tgagaaaccg ctttatcatc cttgtgtatg tactggcagt
     1861 attaagttta tccatcaaga atgcttagtt caatggctga aacacagtcg aaaagaatac
     1921 tgtgaattat gcaagcacag atttgctttt acaccaagcc gcatctacaa gtgcttgttt
     1981 actggctccg tgagctcact actgacgctg ccattagata tgctgtcaac ggaaaatttg
     2041 ttggcagatt gtttgcaggg ttgttttgtg gtgacgtgca cactgtgtgc attcatcagc
     2101 ctggtgtggt tgagagagca gatagtccat gggggagcac caatttggtt ggagcatgct
     2161 gccccaccgt tcaatgctgc ggggcatcac caaaatgagg ctccagcagg aggaaatggt
     2221 gcagaaaatg ttgctgctga tcagcctgct aacccaccag ctgagaacgc agtggtgggg
     2281 gaaaaccctg atgcccagga tgaccaggca gaagaggagg aggaggacaa tgaggaggaa
     2341 gatgacgctg gtgtggagga tgcggcagat gctaataacg gagcccagga tgacatgaat
     2401 tggaatgctt tagaatggga ccgagctgct gaagagctta catgggaaag aatgctagga
     2461 cttgatggat cactagtttt tctggaacat gtcttctggg tggtatcttt aaatacactg
     2521 ttcattcttg tttttgcatt ttgcccttac catattggtc atttctccct tgttggtttg
     2581 ggatttgaag aacacgtcca agcatctcat tttgaaggcc taatcacaac catagttggg
     2641 tatatacttt tagcaataac actgataatt tgtcatggct tggcaactct tgtgaaattt
     2701 catagatctc gtcgcttact gggagtctgc tatattgttg ttaaggtctc tttgttagtg
     2761 gtggtagaaa ttggagtatt ccctctcatt tgtggttggt ggctggatat ctgttccttg
     2821 gaaatgtttg atgctactct gaaagatcga gaactgagct ttcagtcggc tccaggtact
     2881 accatgtttc tgcattggct agtgggaatg gtatatgtct tctactttgc ctccttcatt
     2941 ctactactga gagaggtact tcgacctggt gtcctgtggt ttctaaggaa tttgaatgat
     3001 ccagatttca atccagtaca ggaaatgatc catttgccaa tatataggca tctccgaaga
     3061 tttattttgt cagtgattgt ctttggctcc attgtcctcc tgatgctttg gcttcctata
     3121 cgtataatta agagtgtgct gcctaatttt cttccataca atgtcatgct ctacagtgat
     3181 gctccagtga gtgaactgtc cctcgagctg cttctgcttc aggttgtctt gccagcatta
     3241 ctcgaacagg gacacacgag gcagtggctg aaggggctgg tgcgagcgtg gactgtgacc
     3301 gccggatact tgctggatct tcattcttat ttattgggag accaggaaga aaatgaaaac
     3361 agtgcaaatc aacaagttaa caataatcag catgctcgaa ataacaacgc tattcctgtg
     3421 gtgggagaag gccttcatgc agcccaccaa gccatactcc agcagggagg gcctgttggc
     3481 tttcagcctt accgccgacc tttaaatttt ccactcagga tatttctgtt gattgtcttc
     3541 atgtgtataa cattactgat tgccagcctc atctgcctta ctttaccagt atttgctggc
     3601 cgttggttaa tgtcgttttg gacggggact gccaaaatcc atgagctcta cacagctgct
     3661 tgtggtctct atgtttgctg gctaaccata agggctgtga cggtgatggt ggcatggatg
     3721 cctcagggac gcagagtgat cttccagaag gttaaagagt ggtctctcat gatcatgaag
     3781 actttgatag ttgcggtgct gttggctgga gttgtccctc tccttctggg gctcctgttt
     3841 gagctggtca ttgtggctcc cctgagggtt cccttggatc agactcctct tttttatcca
     3901 tggcaggact gggcacttgg agtcctgcat gccaaaatca ttgcagctat aacattgatg
     3961 ggtcctcagt ggtggttgaa aactgtaatt gaacaggttt acgcaaatgg catccggaac
     4021 attgaccttc actatattgt tcgtaaactg gcagctcccg tgatctctgt gctgttgctt
     4081 tccctgtgtg taccttatgt catagcttct ggtgttgttc ctttactagg tgttactgcg
     4141 gaaatgcaaa acttagtcca tcggcggatt tatccatttt tactgatggt cgtggtattg
     4201 atggcaattt tgtccttcca agtccgccag tttaagcgcc tttatgaaca tattaaaaat
     4261 gacaagtacc ttgtgggtca acgactcgtg aactacgaac ggaaatctgg caaacaaggc
     4321 tcatctccac cacctccaca gtcatcccaa gaataactcg agctagcaga tcttaattaa
     4381 ttaaggatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     4441 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     4501 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     4561 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     4621 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     4681 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     4741 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     4801 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     4861 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4921 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4981 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     5041 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5101 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5161 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5221 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     5281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5341 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     5401 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5461 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5521 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5581 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5641 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5701 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5761 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5821 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5881 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5941 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6001 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6061 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6121 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6181 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6241 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6301 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6361 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6421 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6481 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6541 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6601 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6661 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6721 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6781 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6841 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6901 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6961 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7021 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7081 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7141 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7201 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7261 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7321 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
