LOCUS       V036177                 7279 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036177
VERSION     V036177
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1738..1764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1771..4266
                     /label="KAT2B(NM_003884)"
                     /note="KAT2B(NM_003884)"
                     /gene="KAT2B"
     polyA_signal    4366..4421
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(4782..4798)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4806..4822
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4830..4860)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4875..4896
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        4955..5150
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    5156..5290
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5528..6116)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6287..7147)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7148..7252)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac
     1741 ccatacgatg ttccagatta cgctgaattc atgtccgagg ctggcggggc cgggccgggc
     1801 ggctgcgggg caggagccgg ggcaggggcc gggcccgggg cgctgccccc gcagcctgcg
     1861 gcgcttccgc ccgcgccccc gcagggctcc ccctgcgccg ctgccgccgg gggctcgggc
     1921 gcctgcggtc cggcgacggc agtggctgca gcgggcacgg ccgaaggacc gggaggcggt
     1981 ggctcggccc gaatcgccgt gaagaaagcg caactacgct ccgctccgcg ggccaagaaa
     2041 ctggagaaac tcggagtgta ctccgcctgc aaggccgagg agtcttgtaa atgtaatggc
     2101 tggaaaaacc ctaacccctc acccactccc cccagagccg acctgcagca aataattgtc
     2161 agtctaacag aatcctgtcg gagttgtagc catgccctag ctgctcatgt ttcccacctg
     2221 gagaatgtgt cagaggaaga aatgaacaga ctcctgggaa tagtattgga tgtggaatat
     2281 ctctttacct gtgtccacaa ggaagaagat gcagatacca aacaagttta tttctatcta
     2341 tttaagctct tgagaaagtc tattttacaa agaggaaaac ctgtggttga aggctctttg
     2401 gaaaagaaac ccccatttga aaaacctagc attgaacagg gtgtgaataa ctttgtgcag
     2461 tacaaattta gtcacctgcc agcaaaagaa aggcaaacaa tagttgagtt ggcaaaaatg
     2521 ttcctaaacc gcatcaacta ttggcatctg gaggcaccat ctcaacgaag actgcgatct
     2581 cccaatgatg atatttctgg atacaaagag aactacacaa ggtggctgtg ttactgcaac
     2641 gtgccacagt tctgcgacag tctacctcgg tacgaaacca cacaggtgtt tgggagaaca
     2701 ttgcttcgct cggtcttcac tgttatgagg cgacaactcc tggaacaagc aagacaggaa
     2761 aaagataaac tgcctcttga aaaacgaact ctaatcctca ctcatttccc aaaatttctg
     2821 tccatgctag aagaagaagt atatagtcaa aactctccca tctgggatca ggattttctc
     2881 tcagcctctt ccagaaccag ccagctaggc atccaaacag ttatcaatcc acctcctgtg
     2941 gctgggacaa tttcatacaa ttcaacctca tcttcccttg agcagccaaa cgcagggagc
     3001 agcagtcctg cctgcaaagc ctcttctgga cttgaggcaa acccaggaga aaagaggaaa
     3061 atgactgatt ctcatgttct ggaggaggcc aagaaacccc gagttatggg ggatattccg
     3121 atggaattaa tcaacgaggt tatgtctacc atcacggacc ctgcagcaat gcttggacca
     3181 gagaccaatt ttctgtcagc acactcggcc agggatgagg cggcaaggtt ggaagagcgc
     3241 aggggtgtaa ttgaatttca cgtggttggc aattccctca accagaaacc aaacaagaag
     3301 atcctgatgt ggctggttgg cctacagaac gttttctccc accagctgcc ccgaatgcca
     3361 aaagaataca tcacacggct cgtctttgac ccgaaacaca aaacccttgc tttaattaaa
     3421 gatggccgtg ttattggtgg tatctgtttc cgtatgttcc catctcaagg attcacagag
     3481 attgtcttct gtgctgtaac ctcaaatgag caagtcaagg gctatggaac acacctgatg
     3541 aatcatttga aagaatatca cataaagcat gacatcctga acttcctcac atatgcagat
     3601 gaatatgcaa ttggatactt taagaaacag ggtttctcca aagaaattaa aatacctaaa
     3661 accaaatatg ttggctatat caaggattat gaaggagcca ctttaatggg atgtgagcta
     3721 aatccacgga tcccgtacac agaattttct gtcatcatta aaaagcagaa ggagataatt
     3781 aaaaaactga ttgaaagaaa acaggcacaa attcgaaaag tttaccctgg actttcatgt
     3841 tttaaagatg gagttcgaca gattcctata gaaagcattc ctggaattag agagacaggc
     3901 tggaaaccga gtggaaaaga gaaaagtaaa gagcccagag accctgacca gctttacagc
     3961 acgctcaaga gcatcctcca gcaggtgaag agccatcaaa gcgcttggcc cttcatggaa
     4021 cctgtgaaga gaacagaagc tccaggatat tatgaagtta taaggttccc catggatctg
     4081 aaaaccatga gtgaacgcct caagaatagg tactacgtgt ctaagaaatt attcatggca
     4141 gacttacagc gagtctttac caattgcaaa gagtacaacc cccctgagag tgaatactac
     4201 aaatgtgcca atatcctgga gaaattcttc ttcagtaaaa ttaaggaagc tggattaatt
     4261 gacaagtgac tcgagctagc agatcttaat taattaagga tctttttccc tctgccaaaa
     4321 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     4381 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4441 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     4501 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4561 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4621 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4681 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4741 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4801 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4861 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4921 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
     4981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     5101 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     5161 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5221 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5281 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag
     5461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7261 cccgaaaagt gccacctgg
//
