LOCUS V036177 7279 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V036177 VERSION V036177 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 4..383 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 385..662 /label="chicken β-actin promoter" intron 663..1680 /note="chimera between introns from chicken β-actin and rabbit β-globin" /label="chimeric intron" CDS 1738..1764 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1771..4266 /label="KAT2B(NM_003884)" /note="KAT2B(NM_003884)" /gene="KAT2B" polyA_signal 4366..4421 /note="rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)" /label="β-globin poly(A) signal" primer_bind complement(4782..4798) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4806..4822 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4830..4860) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4875..4896 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 4955..5150 /note="SV40 early promoter" /label="SV40 promoter" polyA_signal 5156..5290 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(5528..6116) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6287..7147) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7148..7252) /gene="bla" /label="AmpR promoter" ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc 541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc 721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg 841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt 901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc 961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg 1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg 1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc 1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc 1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg 1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc 1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg 1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc 1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg 1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt 1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt 1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag 1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac 1741 ccatacgatg ttccagatta cgctgaattc atgtccgagg ctggcggggc cgggccgggc 1801 ggctgcgggg caggagccgg ggcaggggcc gggcccgggg cgctgccccc gcagcctgcg 1861 gcgcttccgc ccgcgccccc gcagggctcc ccctgcgccg ctgccgccgg gggctcgggc 1921 gcctgcggtc cggcgacggc agtggctgca gcgggcacgg ccgaaggacc gggaggcggt 1981 ggctcggccc gaatcgccgt gaagaaagcg caactacgct ccgctccgcg ggccaagaaa 2041 ctggagaaac tcggagtgta ctccgcctgc aaggccgagg agtcttgtaa atgtaatggc 2101 tggaaaaacc ctaacccctc acccactccc cccagagccg acctgcagca aataattgtc 2161 agtctaacag aatcctgtcg gagttgtagc catgccctag ctgctcatgt ttcccacctg 2221 gagaatgtgt cagaggaaga aatgaacaga ctcctgggaa tagtattgga tgtggaatat 2281 ctctttacct gtgtccacaa ggaagaagat gcagatacca aacaagttta tttctatcta 2341 tttaagctct tgagaaagtc tattttacaa agaggaaaac ctgtggttga aggctctttg 2401 gaaaagaaac ccccatttga aaaacctagc attgaacagg gtgtgaataa ctttgtgcag 2461 tacaaattta gtcacctgcc agcaaaagaa aggcaaacaa tagttgagtt ggcaaaaatg 2521 ttcctaaacc gcatcaacta ttggcatctg gaggcaccat ctcaacgaag actgcgatct 2581 cccaatgatg atatttctgg atacaaagag aactacacaa ggtggctgtg ttactgcaac 2641 gtgccacagt tctgcgacag tctacctcgg tacgaaacca cacaggtgtt tgggagaaca 2701 ttgcttcgct cggtcttcac tgttatgagg cgacaactcc tggaacaagc aagacaggaa 2761 aaagataaac tgcctcttga aaaacgaact ctaatcctca ctcatttccc aaaatttctg 2821 tccatgctag aagaagaagt atatagtcaa aactctccca tctgggatca ggattttctc 2881 tcagcctctt ccagaaccag ccagctaggc atccaaacag ttatcaatcc acctcctgtg 2941 gctgggacaa tttcatacaa ttcaacctca tcttcccttg agcagccaaa cgcagggagc 3001 agcagtcctg cctgcaaagc ctcttctgga cttgaggcaa acccaggaga aaagaggaaa 3061 atgactgatt ctcatgttct ggaggaggcc aagaaacccc gagttatggg ggatattccg 3121 atggaattaa tcaacgaggt tatgtctacc atcacggacc ctgcagcaat gcttggacca 3181 gagaccaatt ttctgtcagc acactcggcc agggatgagg cggcaaggtt ggaagagcgc 3241 aggggtgtaa ttgaatttca cgtggttggc aattccctca accagaaacc aaacaagaag 3301 atcctgatgt ggctggttgg cctacagaac gttttctccc accagctgcc ccgaatgcca 3361 aaagaataca tcacacggct cgtctttgac ccgaaacaca aaacccttgc tttaattaaa 3421 gatggccgtg ttattggtgg tatctgtttc cgtatgttcc catctcaagg attcacagag 3481 attgtcttct gtgctgtaac ctcaaatgag caagtcaagg gctatggaac acacctgatg 3541 aatcatttga aagaatatca cataaagcat gacatcctga acttcctcac atatgcagat 3601 gaatatgcaa ttggatactt taagaaacag ggtttctcca aagaaattaa aatacctaaa 3661 accaaatatg ttggctatat caaggattat gaaggagcca ctttaatggg atgtgagcta 3721 aatccacgga tcccgtacac agaattttct gtcatcatta aaaagcagaa ggagataatt 3781 aaaaaactga ttgaaagaaa acaggcacaa attcgaaaag tttaccctgg actttcatgt 3841 tttaaagatg gagttcgaca gattcctata gaaagcattc ctggaattag agagacaggc 3901 tggaaaccga gtggaaaaga gaaaagtaaa gagcccagag accctgacca gctttacagc 3961 acgctcaaga gcatcctcca gcaggtgaag agccatcaaa gcgcttggcc cttcatggaa 4021 cctgtgaaga gaacagaagc tccaggatat tatgaagtta taaggttccc catggatctg 4081 aaaaccatga gtgaacgcct caagaatagg tactacgtgt ctaagaaatt attcatggca 4141 gacttacagc gagtctttac caattgcaaa gagtacaacc cccctgagag tgaatactac 4201 aaatgtgcca atatcctgga gaaattcttc ttcagtaaaa ttaaggaagc tggattaatt 4261 gacaagtgac tcgagctagc agatcttaat taattaagga tctttttccc tctgccaaaa 4321 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta 4381 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga 4441 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc 4501 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc 4561 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta 4621 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac 4681 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat 4741 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg 4801 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 4861 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 4921 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag 4981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 5041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 5101 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt 5161 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5221 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5281 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 5341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 5401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag 5461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 5521 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 5581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 5641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 5701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 5761 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 5821 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 5881 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 5941 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 6001 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 6061 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 6121 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 6181 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 6241 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 6301 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 6361 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 6421 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 6481 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 6541 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 6601 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 6661 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 6721 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 6781 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 6841 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 6901 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 6961 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 7021 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 7081 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 7141 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 7201 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 7261 cccgaaaagt gccacctgg //