LOCUS       V020027                 7868 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020027
VERSION     V020027
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             16..3129
                     /label="IPO7(NM_006391)"
                     /note="IPO7(NM_006391)"
                     /gene="IPO7"
     CDS             3130..3153
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    3225..3280
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(3641..3657)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3665..3681
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3689..3719)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3733..3754
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        3813..4008
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    4014..4148
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4386..4974)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5145..6005)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6006..6110)
                     /gene="bla"
                     /label="AmpR promoter"
     enhancer        6141..6520
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        6522..6799
                     /label="chicken β-actin promoter"
     intron          6800..7817
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
ORIGIN
        1 ttcctcgagg ccaccatgga ccccaacacc attatcgagg ccctgcgggg cactatggac
       61 ccagccctgc gtgaggccgc ggagcgccag ctcaatgaag cacacaagtc tctgaatttt
      121 gtctcaacac tgctccagat tactatgtcg gaacagctgg atttacctgt gagacaggca
      181 ggtgttatct atctgaaaaa tatgataaca cagtattggc ctgatcgaga aacagcacca
      241 ggggatatat ccccttatac tattccagaa gaagatcgcc attgtattcg agaaaatatt
      301 gtagaagcca ttatccattc tcctgagctc atcagggtac agcttactac atgcattcat
      361 cacatcatca aacatgatta tccaagccgc tggactgcca ttgtggacaa aattggcttt
      421 tatcttcagt ccgataacag tgcttgttgg ctaggaattc ttctttgcct ttatcagctt
      481 gtgaaaaatt atgagtataa aaaaccagag gagcggagtc cattggtagc agcaatgcag
      541 cattttctgc cagttctaaa ggatcgtttt atccagcttc tttctgacca gtctgatcag
      601 tctgtcctca tccagaaaca gatattcaag atcttctatg ctcttgttca gtatacacta
      661 ccactggaac tgataaacca acagaacctg acagaatgga tagaaatttt aaagactgtt
      721 gtgaacaggg atgtacctaa tgaaacactt caagttgaag aagatgatcg acctgagtta
      781 ccatggtgga aatgcaagaa gtgggcctta catattttag caagactttt tgaaagatat
      841 ggaagccctg gcaatgtttc caaggagtat aatgaatttg ctgaagtatt tctgaaggca
      901 tttgctgttg gtgtccagca agttttattg aaggtgttat atcagtacaa ggagaagcaa
      961 tatatggctc ctcgagtttt acaacagaca ttaaattata ttaatcaagg agtttctcat
     1021 gctctcacct ggaagaatct gaagccccat atacaaggca ttatccaaga tgttattttt
     1081 ccattgatgt gctatacaga tgctgatgag gaactttggc aagaagaccc ttacgaatat
     1141 atacgcatga agtttgatgt gtttgaagat ttcatttctc ctaccactgc tgcccagaca
     1201 cttttgttta cagcctgtag taagaggaaa gaggtactgc aaaagactat gggattttgt
     1261 taccagattc ttacagaacc aaatgctgac cctcgaaaaa aagatggagc cctgcatatg
     1321 attggctctt tagctgaaat acttctgaag aaaaagatct ataaagatca gatggaatac
     1381 atgttgcaga atcatgtatt ccctctcttc agcagtgaac taggctacat gagagcaagg
     1441 gcttgctggg tacttcacta tttttgtgaa gtgaagttca aaagtgatca gaaccttcaa
     1501 acagccttag agctaacaag aagatgtctg attgatgata gagaaatgcc tgtgaaagtg
     1561 gaagctgcca ttgcccttca agtattgatc agcaatcaag aaaaagctaa agaatatatc
     1621 acaccattca tcagacctgt aatgcaggct cttcttcaca ttataagaga aacagaaaat
     1681 gatgacctta ccaatgtaat tcagaaaatg atctgtgaat atagtgaaga agttactcct
     1741 attgcagtag aaatgacaca acatttggca atgacattta accaagtaat ccagacgggg
     1801 ccagatgaag aaggtagtga tgacaaagca gttactgcta tgggaattct gaatacaatt
     1861 gatacacttc ttagtgtagt tgaagatcat aaagagataa cccaacagct tgagggaatc
     1921 tgcttacagg tcattggtac tgttttacaa cagcatgtct tagaattcta tgaggagatc
     1981 ttctctttag cgcacagttt gacatgtcaa caagtgtctc cacagatgtg gcagctacta
     2041 ccccttgtat ttgaagtctt tcagcaagat ggctttgatt actttacaga tatgatgccc
     2101 ctccttcata attatgtaac agttgataca gacacacttc tgtctgacac caagtatctt
     2161 gaaatgatat acagtatgtg caaaaaggtt cttacaggag ttgcaggaga agatgcagag
     2221 tgtcatgcag caaaattgtt agaggtcatc attctgcagt gcaaagggcg tggcattgac
     2281 cagtgcattc ccttattcgt ggaagcagcc ttagaaagac tgacaagaga ggttaagaca
     2341 agtgaacttc gaactatgtg tctgcaagtt gcaattgcag ctttgtatta taatccacac
     2401 ctactactca ataccttaga aaatcttcgc ttccctaata atgttgaacc agttacaaat
     2461 cattttatta cacagtggct taatgatgtt gactgtttct tggggcttca tgacagaaag
     2521 atgtgtgttc tcggactctg tgctcttatt gatatggaac agatacccca agttttaaat
     2581 caggtttctg gacagatttt gccggctttt atccttttat ttaacggatt gaaaagagca
     2641 tatgcctgcc atgcagaaca tgagaatgac agtgatgatg atgatgaagc tgaagatgat
     2701 gatgaaaccg aggaactggg gagtgatgaa gatgatattg atgaagatgg gcaagaatat
     2761 ttggagattc tggctaagca ggctggtgaa gatggagatg atgaagattg ggaagaagat
     2821 gatgctgaag agactgctct ggaaggctat tccacaatca ttgatgatga agataaccct
     2881 gttgatgagt atcagatatt taaagctatc tttcaaacta ttcaaaatcg taatcctgtg
     2941 tggtatcagg cactgactca cggtcttaat gaagaacaaa gaaaacagtt acaggacata
     3001 gcaactctgg ctgatcaaag aagagcagcc catgaatcca aaatgattga gaagcatgga
     3061 ggatacaaat tcagtgctcc agttgtgcca agttctttca attttggagg cccagcacca
     3121 gggatgaatg attacaagga cgacgatgac aagtagagat ctttttccct ctgccaaaaa
     3181 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3241 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3301 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     3361 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     3421 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     3481 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     3541 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     3601 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     3661 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3721 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3781 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     4021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4141 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     4321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6121 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     6181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     6241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     6301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     6361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     6421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     6481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     6541 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     6601 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     6661 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     6721 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     6781 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     6841 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     6901 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     6961 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     7021 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     7081 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     7141 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     7201 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     7261 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     7321 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     7381 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     7441 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     7501 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     7561 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     7621 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     7681 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     7741 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     7801 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     7861 gcaaagaa
//
