LOCUS V020027 7868 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020027 VERSION V020027 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 16..3129 /label="IPO7(NM_006391)" /note="IPO7(NM_006391)" /gene="IPO7" CDS 3130..3153 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 3225..3280 /note="rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)" /label="β-globin poly(A) signal" primer_bind complement(3641..3657) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3665..3681 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3689..3719) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3733..3754 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 3813..4008 /note="SV40 early promoter" /label="SV40 promoter" polyA_signal 4014..4148 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4386..4974) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5145..6005) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6006..6110) /gene="bla" /label="AmpR promoter" enhancer 6141..6520 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 6522..6799 /label="chicken β-actin promoter" intron 6800..7817 /note="chimera between introns from chicken β-actin and rabbit β-globin" /label="chimeric intron" ORIGIN 1 ttcctcgagg ccaccatgga ccccaacacc attatcgagg ccctgcgggg cactatggac 61 ccagccctgc gtgaggccgc ggagcgccag ctcaatgaag cacacaagtc tctgaatttt 121 gtctcaacac tgctccagat tactatgtcg gaacagctgg atttacctgt gagacaggca 181 ggtgttatct atctgaaaaa tatgataaca cagtattggc ctgatcgaga aacagcacca 241 ggggatatat ccccttatac tattccagaa gaagatcgcc attgtattcg agaaaatatt 301 gtagaagcca ttatccattc tcctgagctc atcagggtac agcttactac atgcattcat 361 cacatcatca aacatgatta tccaagccgc tggactgcca ttgtggacaa aattggcttt 421 tatcttcagt ccgataacag tgcttgttgg ctaggaattc ttctttgcct ttatcagctt 481 gtgaaaaatt atgagtataa aaaaccagag gagcggagtc cattggtagc agcaatgcag 541 cattttctgc cagttctaaa ggatcgtttt atccagcttc tttctgacca gtctgatcag 601 tctgtcctca tccagaaaca gatattcaag atcttctatg ctcttgttca gtatacacta 661 ccactggaac tgataaacca acagaacctg acagaatgga tagaaatttt aaagactgtt 721 gtgaacaggg atgtacctaa tgaaacactt caagttgaag aagatgatcg acctgagtta 781 ccatggtgga aatgcaagaa gtgggcctta catattttag caagactttt tgaaagatat 841 ggaagccctg gcaatgtttc caaggagtat aatgaatttg ctgaagtatt tctgaaggca 901 tttgctgttg gtgtccagca agttttattg aaggtgttat atcagtacaa ggagaagcaa 961 tatatggctc ctcgagtttt acaacagaca ttaaattata ttaatcaagg agtttctcat 1021 gctctcacct ggaagaatct gaagccccat atacaaggca ttatccaaga tgttattttt 1081 ccattgatgt gctatacaga tgctgatgag gaactttggc aagaagaccc ttacgaatat 1141 atacgcatga agtttgatgt gtttgaagat ttcatttctc ctaccactgc tgcccagaca 1201 cttttgttta cagcctgtag taagaggaaa gaggtactgc aaaagactat gggattttgt 1261 taccagattc ttacagaacc aaatgctgac cctcgaaaaa aagatggagc cctgcatatg 1321 attggctctt tagctgaaat acttctgaag aaaaagatct ataaagatca gatggaatac 1381 atgttgcaga atcatgtatt ccctctcttc agcagtgaac taggctacat gagagcaagg 1441 gcttgctggg tacttcacta tttttgtgaa gtgaagttca aaagtgatca gaaccttcaa 1501 acagccttag agctaacaag aagatgtctg attgatgata gagaaatgcc tgtgaaagtg 1561 gaagctgcca ttgcccttca agtattgatc agcaatcaag aaaaagctaa agaatatatc 1621 acaccattca tcagacctgt aatgcaggct cttcttcaca ttataagaga aacagaaaat 1681 gatgacctta ccaatgtaat tcagaaaatg atctgtgaat atagtgaaga agttactcct 1741 attgcagtag aaatgacaca acatttggca atgacattta accaagtaat ccagacgggg 1801 ccagatgaag aaggtagtga tgacaaagca gttactgcta tgggaattct gaatacaatt 1861 gatacacttc ttagtgtagt tgaagatcat aaagagataa cccaacagct tgagggaatc 1921 tgcttacagg tcattggtac tgttttacaa cagcatgtct tagaattcta tgaggagatc 1981 ttctctttag cgcacagttt gacatgtcaa caagtgtctc cacagatgtg gcagctacta 2041 ccccttgtat ttgaagtctt tcagcaagat ggctttgatt actttacaga tatgatgccc 2101 ctccttcata attatgtaac agttgataca gacacacttc tgtctgacac caagtatctt 2161 gaaatgatat acagtatgtg caaaaaggtt cttacaggag ttgcaggaga agatgcagag 2221 tgtcatgcag caaaattgtt agaggtcatc attctgcagt gcaaagggcg tggcattgac 2281 cagtgcattc ccttattcgt ggaagcagcc ttagaaagac tgacaagaga ggttaagaca 2341 agtgaacttc gaactatgtg tctgcaagtt gcaattgcag ctttgtatta taatccacac 2401 ctactactca ataccttaga aaatcttcgc ttccctaata atgttgaacc agttacaaat 2461 cattttatta cacagtggct taatgatgtt gactgtttct tggggcttca tgacagaaag 2521 atgtgtgttc tcggactctg tgctcttatt gatatggaac agatacccca agttttaaat 2581 caggtttctg gacagatttt gccggctttt atccttttat ttaacggatt gaaaagagca 2641 tatgcctgcc atgcagaaca tgagaatgac agtgatgatg atgatgaagc tgaagatgat 2701 gatgaaaccg aggaactggg gagtgatgaa gatgatattg atgaagatgg gcaagaatat 2761 ttggagattc tggctaagca ggctggtgaa gatggagatg atgaagattg ggaagaagat 2821 gatgctgaag agactgctct ggaaggctat tccacaatca ttgatgatga agataaccct 2881 gttgatgagt atcagatatt taaagctatc tttcaaacta ttcaaaatcg taatcctgtg 2941 tggtatcagg cactgactca cggtcttaat gaagaacaaa gaaaacagtt acaggacata 3001 gcaactctgg ctgatcaaag aagagcagcc catgaatcca aaatgattga gaagcatgga 3061 ggatacaaat tcagtgctcc agttgtgcca agttctttca attttggagg cccagcacca 3121 gggatgaatg attacaagga cgacgatgac aagtagagat ctttttccct ctgccaaaaa 3181 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 3241 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 3301 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 3361 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 3421 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 3481 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 3541 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 3601 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 3661 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 3721 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 3781 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 3841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 3901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 3961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 4021 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 4081 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 4141 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 4201 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 4261 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 4321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 4501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 4561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 4621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 4681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 4801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 4861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 5041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 5101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 5161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 5221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 5281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 5341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 5401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 5461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 5521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 5581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 5641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 5701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 5761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 5821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 5881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 5941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 6001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 6061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 6121 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 6181 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 6241 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 6301 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 6361 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 6421 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 6481 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 6541 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 6601 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 6661 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 6721 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 6781 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 6841 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 6901 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 6961 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 7021 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 7081 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 7141 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 7201 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 7261 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 7321 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 7381 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 7441 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 7501 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 7561 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 7621 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 7681 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 7741 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 7801 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 7861 gcaaagaa //