LOCUS V020032 8045 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020032 VERSION V020032 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS 16..3306 /label="IPO5(NM_002271)" /note="IPO5(NM_002271)" /gene="IPO5" CDS 3307..3330 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 3402..3457 /note="rabbit β-globin polyadenylation signal (Gil and Proudfoot, 1987)" /label="β-globin poly(A) signal" primer_bind complement(3818..3834) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3842..3858 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3866..3896) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3910..3931 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 3990..4185 /note="SV40 early promoter" /label="SV40 promoter" polyA_signal 4191..4325 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4563..5151) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5322..6182) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6183..6287) /gene="bla" /label="AmpR promoter" enhancer 6318..6697 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 6699..6976 /label="chicken β-actin promoter" intron 6977..7994 /note="chimera between introns from chicken β-actin and rabbit β-globin" /label="chimeric intron" ORIGIN 1 ttcctcgagg ccaccatggc ggcggccgcg gcggagcagc aacagttcta cctgctcctg 61 ggaaacctgc tcagccccga caatgtggtc cggaaacagg cagaggaaac ctatgagaat 121 atcccaggcc agtcaaagat cacattcctc ttacaagcca tcagaaatac aacagctgct 181 gaagaggcta gacaaatggc cgccgttctc ctaagacgtc tcttgtcctc tgcatttgat 241 gaagtctatc cagcacttcc ctctgatgtt cagactgcca tcaagagtga gctactcatg 301 attattcaga tggaaacaca atctagcatg aggaaaaaag tttgtgatat tgcggcagaa 361 ctggccagga atttaataga tgaggatggc aataaccagt ggcccgaagg tttgaagttc 421 ctttttgatt cagtcagctc tcaaaatgtg ggactgcggg aagctgccct tcacattttc 481 tggaactttc ctggaatttt tgggaaccag caacaacact atttagatgt catcaaacga 541 atgttagttc agtgtatgca agatcaggaa cacccgtcga tcaggacgtt atctgctaga 601 gctacagctg catttatact tgcaaatgag cataatgttg ctctgttcaa acattttgca 661 gacttgctac cgggattcct acaggcggta aatgactcgt gctaccagaa tgatgattct 721 gtcctaaaat ccctcgttga gattgcagat actgttccaa agtatttgcg tcctcacttg 781 gaagcaactc tacagctaag tctaaagttg tgtggagaca ctagcctcaa caatatgcaa 841 cgccagcttg cccttgaagt gatcgtcacc ctctctgaga ctgcagctgc tatgttaaga 901 aaacatacca atattgttgc acagactatt cctcagatgt tagcaatgat ggttgatttg 961 gaagaagatg aggactgggc aaatgcagat gaactagaag atgatgattt tgacagcaat 1021 gcagttgcag gcgagagtgc tctagatcga atggcttgcg gacttggtgg aaagctcgtt 1081 ctgccgatga tcaaggaaca cattatgcaa atgcttcaaa atcctgactg gaaataccgg 1141 catgcaggat tgatggcctt atctgccatt ggtgaagggt gccaccagca aatggaagga 1201 attctaaatg agatcgtaaa ttttgtttta ctttttctcc aggatcctca tccaagagta 1261 aggtatgcag cctgtaatgc cgtgggacag atggctacag attttgcacc tggtttccaa 1321 aagaaatttc atgagaaggt gattgcagct ctgctgcaga ccatggaaga ccaaggcaat 1381 caacgtgtgc aggcccatgc agctgctgcc ctcattaact ttactgaaga ctgtcccaag 1441 tcactactta ttccatactt ggataatttg gtgaaacatc tgcattccat tatggtactg 1501 aagcttcaag agctgattca gaaaggcacc aagttagttt tggaacaagt tgtgacatcc 1561 attgcatcag ttgccgatac tgcagaagaa aaatttgtcc cctactatga tttatttatg 1621 ccatcactga agcacatcgt tgagaatgcg gttcaaaaag aactgagact tctgagagga 1681 aaaactattg aatgcattag cctcattggt ctggctgttg ggaaggaaaa attcatgcag 1741 gatgcatcag atgtgatgca gcttttgtta aagacccaga cagacttcaa tgatatggaa 1801 gatgatgatc ctcagatctc ttacatgatc tcagcatggg ccagaatgtg caaaatcctt 1861 ggaaaagaat ttcagcaata ccttccagtg gttatggggc ctttaatgaa gacggcttca 1921 attaagcccg aagtagccct tttagatacc caagacatgg agaatatgag tgatgatgat 1981 ggttgggaat ttgtgaacct tggagatcag caaagctttg gtattaaaac tgcaggacta 2041 gaagaaaaat caactgcttg ccagatgttg gtttgctatg ctaaggagtt aaaggaaggc 2101 tttgtggagt acaccgaaca ggttgtcaaa ctgatggtcc ctttactgaa attttatttc 2161 cacgatggtg ttcgagtggc agcagcggaa tccatgcctc ttctcctgga gtgtgcaaga 2221 gtccgtggtc ctgagtatct cacacagatg tggcatttta tgtgtgatgc tctaattaag 2281 gccattggta cagaaccaga ttcagacgtc ctctcagaaa taatgcattc ttttgcaaag 2341 tgcattgaag taatgggaga tggatgcctt aataatgaac actttgaaga actgggaggt 2401 atattgaaag caaagcttga agaacatttt aaaaatcaag aattacgaca agttaaaaga 2461 caagatgaag actatgatga acaggtcgaa gagtcactac aagatgagga tgataatgat 2521 gtttatattc tgaccaaagt gtcagatatt ttacactcaa tattcagtag ctacaaagaa 2581 aaggtgttac catggtttga acagctgctt ccattaattg tcaacctcat ttgtccacat 2641 agaccatggc cagacagaca atggggatta tgcatctttg atgatgtcat agaacactgt 2701 agtccagcct catttaaata cgcagaatat ttcttaagac caatgctcca atatgtatgt 2761 gacaacagcc cagaagtcag gcaagcagct gcatatggcc tgggagtcat ggcacagtac 2821 ggtggagata attatcgccc tttttgtaca gaagcacttc ccctgctggt aagagttatt 2881 cagtctgcgg attctaagac caaagaaaat gtcaatgcta cagagaactg catctcagca 2941 gtagggaaaa tcatgaagtt caagcctgac tgtgtaaacg ttgaagaggt ccttccacac 3001 tggttgtctt ggcttccact acatgaagat aaagaagaag ctgttcagac tttcaattat 3061 ctgtgtgacc tgattgaaag taatcatcca attgttcttg gcccaaacaa taccaatctg 3121 cccaaaatat ttagtataat tgcggaagga gaaatgcacg aggcaattaa acatgaagat 3181 ccttgtgcca aacgtctggc caatgtcgtt cgccaagtac agacttctgg aggactgtgg 3241 actgagtgca tagcacagct cagtcctgag cagcaggccg ccattcagga gctcctgaac 3301 tctgcggatt acaaggacga cgatgacaag tagagatctt tttccctctg ccaaaaatta 3361 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt 3421 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc 3481 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat 3541 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc 3601 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt 3661 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc 3721 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag 3781 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 3841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 3901 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 3961 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg 4021 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 4081 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 4141 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta 4201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 4261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 4321 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag 4501 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 4561 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 4621 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 4681 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 4741 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 4801 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 4861 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 4921 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 4981 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 5041 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5101 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 5161 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 5221 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 5281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 5341 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 5401 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 5461 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 5521 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 5581 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 5641 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 5701 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 5761 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 5821 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 5881 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 5941 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 6001 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 6061 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 6121 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 6181 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 6241 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 6301 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg 6361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 6421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 6481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 6541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga 6601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg 6661 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg 6721 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta 6781 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg 6841 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg 6901 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg 6961 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc 7021 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg 7081 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt 7141 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg 7201 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc 7261 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga 7321 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg 7381 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct 7441 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg 7501 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg 7561 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg 7621 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt 7681 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg 7741 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga 7801 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc 7861 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt 7921 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct 7981 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca 8041 aagaa //