LOCUS       40924_8406        5712 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5712)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5712)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gap             14..23
                     /estimated_length=10
     CDS             24..737
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     misc_feature    738..803
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1069..1124
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1485..1501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1509..1525)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1533..1563)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1577..1598)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1656..1852
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1858..1992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2230..2818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2992..3849)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3850..3954)
                     /label=AmpR promoter
     enhancer        3985..4364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3985..4362
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4366..4643
                     /label=chicken beta-actin promoter
     intron          4644..5661
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaca tgannnnnnn nnnatgggta aaggagaaga acttttcact ggagttgtcc
       61 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      121 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      181 tacctgttcc atggccaaca cttgtcacta ctttcacnta tggtgttcaa tgcttttcaa
      241 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
      301 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      361 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      421 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      481 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      541 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      601 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      661 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      721 tggatgaact atacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
      781 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
      841 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      901 ataaaatgaa tgnnntcgag gaattcactc ctcaggtgca ggctgcctat cagaaggtgg
      961 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
     1021 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     1081 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     1141 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     1201 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     1261 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     1321 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     1381 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     1441 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     1501 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1561 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1621 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     1681 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1741 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     1801 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     1861 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1921 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1981 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc
     2161 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2221 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2281 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2341 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2401 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2461 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2521 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2581 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2641 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2701 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2761 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2821 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2881 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2941 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3001 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3061 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3121 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3181 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3241 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3301 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3361 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3421 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3481 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3541 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3601 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3661 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3721 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3781 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3841 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3901 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3961 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
     4021 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     4081 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     4141 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     4201 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     4261 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     4321 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     4381 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     4441 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg
     4501 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc
     4561 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
     4621 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct
     4681 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
     4741 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
     4801 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
     4861 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg
     4921 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg
     4981 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga
     5041 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg
     5101 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg
     5161 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc
     5221 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg
     5281 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     5341 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     5401 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     5461 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     5521 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     5581 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     5641 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     5701 tttggcaaag aa
//
