LOCUS       V038275                 7189 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038275
VERSION     V038275
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1741..4122
                     /label="FURIN(NM_002569)"
                     /note="FURIN(NM_002569)"
                     /gene="FURIN"
     CDS             4129..4194
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    4277..4332
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(4693..4709)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4717..4733
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4741..4771)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4785..4806
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        4865..5060
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    5066..5200
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5438..6026)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6197..7057)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7058..7162)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccacc
     1741 atggagctga ggccctggtt gctatgggtg gtagcagcaa caggaacctt ggtcctgcta
     1801 gcagctgatg ctcagggcca gaaggtcttc accaacacgt gggctgtgcg catccctgga
     1861 ggcccagcgg tggccaacag tgtggcacgg aagcatgggt tcctcaacct gggccagatc
     1921 ttcggggact attaccactt ctggcatcga ggagtgacga agcggtccct gtcgcctcac
     1981 cgcccgcggc acagccggct gcagagggag cctcaagtac agtggctgga acagcaggtg
     2041 gcaaagcgac ggactaaacg ggacgtgtac caggagccca cagaccccaa gtttcctcag
     2101 cagtggtacc tgtctggtgt cactcagcgg gacctgaatg tgaaggcggc ctgggcgcag
     2161 ggctacacag ggcacggcat tgtggtctcc attctggacg atggcatcga gaagaaccac
     2221 ccggacttgg caggcaatta tgatcctggg gccagttttg atgtcaatga ccaggaccct
     2281 gacccccagc ctcggtacac acagatgaat gacaacaggc acggcacacg gtgtgcgggg
     2341 gaagtggctg cggtggccaa caacggtgtc tgtggtgtag gtgtggccta caacgcccgc
     2401 attggagggg tgcgcatgct ggatggcgag gtgacagatg cagtggaggc acgctcgctg
     2461 ggcctgaacc ccaaccacat ccacatctac agtgccagct ggggccccga ggatgacggc
     2521 aagacagtgg atgggccagc ccgcctcgcc gaggaggcct tcttccgtgg ggttagccag
     2581 ggccgagggg ggctgggctc catctttgtc tgggcctcgg ggaacggggg ccgggaacat
     2641 gacagctgca actgcgacgg ctacaccaac agtatctaca cgctgtccat cagcagcgcc
     2701 acgcagtttg gcaacgtgcc gtggtacagc gaggcctgct cgtccacact ggccacgacc
     2761 tacagcagtg gcaaccagaa tgagaagcag atcgtgacga ctgacttgcg gcagaagtgc
     2821 acggagtctc acacgggcac ctcagcctct gcccccttag cagccggcat cattgctctc
     2881 accctggagg ccaataagaa cctcacatgg cgggacatgc aacacctggt ggtacagacc
     2941 tcgaagccag cccacctcaa tgccaacgac tgggccacca atggtgtggg ccggaaagtg
     3001 agccactcat atggctacgg gcttttggac gcaggcgcca tggtggccct ggcccagaat
     3061 tggaccacag tggcccccca gcggaagtgc atcatcgaca tcctcaccga gcccaaagac
     3121 atcgggaaac ggctcgaggt gcggaagacc gtgaccgcgt gcctgggcga gcccaaccac
     3181 atcactcggc tggagcacgc tcaggcgcgg ctcaccctgt cctataatcg ccgtggcgac
     3241 ctggccatcc acctggtcag ccccatgggc acccgctcca ccctgctggc agccaggcca
     3301 catgactact ccgcagatgg gtttaatgac tgggccttca tgacaactca ttcctgggat
     3361 gaggatccct ctggcgagtg ggtcctagag attgaaaaca ccagcgaagc caacaactat
     3421 gggacgctga ccaagttcac cctcgtactc tatggcaccg cccctgaggg gctgcccgta
     3481 cctccagaaa gcagtggctg caagaccctc acgtccagtc aggcctgtgt ggtgtgcgag
     3541 gaaggcttct ccctgcacca gaagagctgt gtccagcact gccctccagg gttcgccccc
     3601 caagtcctcg atacgcacta tagcaccgag aatgacgtgg agaccatccg ggccagcgtc
     3661 tgcgccccct gccacgcctc atgtgccaca tgccaggggc cggccctgac agactgcctc
     3721 agctgcccca gccacgcctc cttggaccct gtggagcaga cttgctcccg gcaaagccag
     3781 agcagccgag agtccccgcc acagcagcag ccacctcggc tgcccccgga ggtggaggcg
     3841 gggcaacggc tgcgggcagg gctgctgccc tcacacctgc ctgaggtggt ggccggcctc
     3901 agctgcgcct tcatcgtgct ggtcttcgtc actgtcttcc tggtcctgca gctgcgctct
     3961 ggctttagtt ttcggggggt gaaggtgtac accatggacc gtggcctcat ctcctacaag
     4021 gggctgcccc ctgaagcctg gcaggaggag tgcccgtctg actcagaaga ggacgagggc
     4081 cggggcgaga ggaccgcctt tatcaaagac cagagcgccc tcggtaccga ctacaaagac
     4141 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagctc
     4201 gagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag
     4261 catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt
     4321 ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag
     4381 tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg
     4441 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     4501 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     4561 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     4621 ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt
     4681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4741 caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc
     4801 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     4861 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4921 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4981 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5041 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     5101 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5161 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     5221 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5281 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5341 cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5401 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5461 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5521 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5581 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5641 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5701 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5761 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5821 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5881 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5941 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6001 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6061 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6121 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6181 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6241 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6301 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6361 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6421 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6481 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6541 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6601 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6661 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6721 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6781 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6841 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6901 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6961 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7021 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7081 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7141 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgg
//
