LOCUS       40924_8401        5332 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAGmA20f8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5332)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             49..606
                     /codon_start=1
                     /note="unnamed protein product; mA20 (591-775)"
                     /protein_id="SJL88084.1"
                     /translation="KCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASAKAGSPAPR
                     FQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDMPRTTQ
                     VASRPKCARASCKNILACRSEELCMECQHLSQRVGSVAHRGEPTPEEPPKQRCRAPACD
                     HFGNAKCNGYCNECYQFKQMYG"
     polyA_signal    685..740
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1101..1117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1125..1141)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1149..1179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1193..1214)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1272..1468
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1474..1608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1846..2434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2608..3465)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3466..3570)
                     /label=AmpR promoter
     enhancer        3605..3984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3605..3982
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        3986..4263
                     /label=chicken beta-actin promoter
     intron          4264..5281
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatgaa gtgcaggaaa
       61 gctggctgca tgtattttgg gactccagaa aacaagggct tttgcactct atgtttcatc
      121 gaatacagag aaaataagca gtctgttact gcctctgcga aagctggttc cccggccccc
      181 aggttccaga acaatgtccc gtgcctgggc agggagtgcg gcacactcgg aagcaccatg
      241 tttgaagggt actgtcagaa gtgtttcatc gaagctcaga accagagatt ccatgaagca
      301 agaagaacgg aagaacagct gagatcaagc cagcatagag acatgcctcg aactacacag
      361 gtagcctcaa ggccgaaatg tgcccgggcc tcctgcaaga acattctggc ctgtcgcagt
      421 gaggaactct gtatggagtg ccagcaccta agccaacgag taggttctgt ggcccaccgg
      481 ggtgagccca cgcctgaaga gccccctaaa cagcgctgcc gggcccctgc ttgtgatcac
      541 tttggcaatg ccaagtgtaa tggttactgc aatgagtgct accagttcaa gcagatgtat
      601 ggctaagtgt cgacgaagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
      661 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
      721 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
      781 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
      841 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
      901 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
      961 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     1021 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     1081 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1141 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga
     1201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1261 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     1321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     1381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1441 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     1501 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1561 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
     1621 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1681 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1741 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg
     1801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2281 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2521 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2581 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2641 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2701 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2761 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2821 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2881 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2941 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3001 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3061 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3121 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3181 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3241 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3301 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3361 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3421 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3481 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3541 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     3601 gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3661 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3781 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3841 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3901 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3961 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     4021 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     4081 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
     4141 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4201 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4261 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
     4321 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     4381 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     4441 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     4501 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     4561 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     4621 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     4681 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     4741 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     4801 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     4861 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
     4921 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     4981 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     5041 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     5101 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     5161 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     5221 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     5281 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//
