LOCUS       40924_8391        6871 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAGmA20f6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6871)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             46..2142
                     /codon_start=1
                     /note="unnamed protein product; mA20 (1-699)"
                     /protein_id="SJL88016.1"
                     /translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMHR
                     YTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGNCL
                     MHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRN
                     WNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSLESGS
                     NFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLVNRDRG
                     RFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEANLPKEIN
                     LVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNCPFFMSVN
                     TQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPHSAPPTAPS
                     LFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQACLQDVTRTF
                     NGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHRTGNVSPSGCL
                     SQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVTASAKAGSPAPR
                     FQNNVPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEARRTEEQLRSSQHRDMPRTTQ
                     VASRL"
     polyA_signal    2224..2279
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2640..2656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2664..2680)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2688..2718)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2732..2753)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2811..3007
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    3013..3147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3385..3973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4147..5004)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5005..5109)
                     /label=AmpR promoter
     enhancer        5144..5523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5144..5521
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5525..5802
                     /label=chicken beta-actin promoter
     intron          5803..6820
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
       61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
      121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
      181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
      241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
      301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
      361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
      421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
      481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
      541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
      601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
      661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
      721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
      781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
      841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
      901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
      961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
     1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
     1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
     1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata
     1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac
     1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta
     1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc
     1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc
     1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga
     1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag
     1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc
     1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac
     1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac
     1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac
     1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac
     1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc
     1921 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg
     1981 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa
     2041 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag
     2101 catagagaca tgcctcgaac tacacaggta gcctcaaggc tgtaagtgtc gacgaagatc
     2161 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     2221 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     2281 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     2341 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     2401 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     2461 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     2521 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     2581 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     2641 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2701 ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2761 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa
     2821 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     2881 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     2941 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3001 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3061 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3121 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     3181 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3241 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3301 aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3781 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3841 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3901 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3961 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4021 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4081 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4141 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4201 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4261 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4321 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4381 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4441 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4501 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4561 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4621 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4681 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4741 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4801 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4861 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4921 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4981 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5041 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5101 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg attattgact
     5161 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     5221 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     5281 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     5341 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     5401 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     5461 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     5521 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc
     5581 ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg
     5641 ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa
     5701 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     5761 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc
     5821 cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
     5881 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
     5941 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
     6001 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
     6061 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
     6121 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
     6181 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
     6241 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
     6301 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
     6361 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
     6421 gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc
     6481 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     6541 ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     6601 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     6661 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     6721 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     6781 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     6841 tattgtgctg tctcatcatt ttggcaaaga a
//
