LOCUS       40924_8381        5872 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAGmA20f3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5872)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             46..1143
                     /codon_start=1
                     /note="unnamed protein product; mA20 (1-366)"
                     /protein_id="SJL88009.1"
                     /translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMHR
                     YTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGNCL
                     MHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYDTRN
                     WNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSLESGS
                     NFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLVNRDRG
                     RFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEANLPKEIN
                     LVDDYFELVQHEYKKWQENSDQARRA"
     polyA_signal    1225..1280
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1641..1657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1665..1681)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1689..1719)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1733..1754)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1812..2008
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    2014..2148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2386..2974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3148..4005)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4006..4110)
                     /label=AmpR promoter
     enhancer        4145..4524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4145..4522
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4526..4803
                     /label=chicken beta-actin promoter
     intron          4804..5821
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
       61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
      121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
      181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
      241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
      301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
      361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
      421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
      481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
      541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
      601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
      661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
      721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
      781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
      841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
      901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
      961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
     1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
     1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
     1141 gcgtaagtgt cgacgaagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     1201 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     1261 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     1321 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     1381 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     1441 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     1501 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     1561 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     1621 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1681 attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc taatgagtga
     1741 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1801 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     1861 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     1921 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     1981 cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt
     2041 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2101 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat
     2161 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2221 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2281 cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt gagcaaaagg
     2341 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2401 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2461 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2521 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2581 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2641 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2701 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2761 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2821 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2881 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2941 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3001 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3061 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4081 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     4141 gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4201 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4261 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4321 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4381 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4441 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4501 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     4561 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     4621 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
     4681 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4741 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4801 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
     4861 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     4921 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     4981 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     5041 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     5101 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     5161 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     5221 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     5281 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5341 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     5401 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
     5461 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     5521 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     5581 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     5641 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     5701 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     5761 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     5821 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//
