LOCUS       V008844                 5678 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008844
VERSION     V008844
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5678)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5678)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             43..948
                     /gene="HNRNPC"
                     /label="Heterogeneous nuclear ribonucleoprotein C"
                     /note="Heterogeneous nuclear ribonucleoprotein C from Pongo
                     abelii. Accession#: Q5RA82"
     polyA_signal    1031..1086
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1447..1463)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1471..1487)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1495..1525)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1539..1560)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1618..1814
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1820..1954
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2192..2780)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2954..3811)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3812..3916)
                     /label="AmpR promoter"
     enhancer        3951..4330
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3951..4328
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4332..4609
                     /label="chicken beta-actin promoter"
     intron          4610..5627
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggccag caacgttacc
       61 aacaagacag atcctcgctc catgaactcc cgtgtattca ttgggaatct caacactctt
      121 gtggtcaaga aatctgatgt ggaggcaatc ttttcgaagt atggcaaaat tgtgggctgc
      181 tctgttcata agggctttgc cttcgttcag tatgttaatg agagaaatgc ccgggctgct
      241 gtagcaggag aggatggcag aatgattgct ggccaggttt tagatattaa cctggctgca
      301 gagccaaaag tgaaccgagg aaaagcaggt gtgaaacgat ctgcagcgga gatgtacggg
      361 tcagtaacag aacacccttc tccgtcccct ctactcagct cctcttttga cttggactat
      421 gactttcaac gggactatta tgataggatg tacagttacc cagcacgtgt acctcctcct
      481 cctcctattg ctcgggctgt agtgccctcg aaacgtcagc gtgtatcagg aaacacttca
      541 cgaaggggca aaagtggctt caattctaag agtggacagc ggggatcttc caagtctgga
      601 aagttgaaag gagatgacct tcaggccatt aagaaggagc tgacccagat aaaacaaaaa
      661 gtggattctc tcctggaaaa cctggaaaaa atggaaaagg aacagagcaa acaagcagta
      721 gagatgaaga atgataagtc agaagaggag cagagcagca gctccgtgaa gaaagatgag
      781 actaatgtga agatggagtc tgaggggggt gcagatgact ctgctgagga gggggaccta
      841 ctggatgatg atgataatga agatcggggg gatgaccagc tggagttgat caaggatgat
      901 gaaaaagagg ctgaggaagg agaggatgac agagacagcg ccaatggagg atgactcgac
      961 gaagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     1021 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     1081 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     1141 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     1201 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     1261 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     1321 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     1381 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta
     1441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     1501 acgagccgga agcataaagt gtaaagcctg ggtgcctaat gagtgagcta actcacatta
     1561 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc
     1621 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     1681 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1741 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1801 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa
     1861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1921 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc
     1981 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2101 tccacagaat cagggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgatc gacattgatt
     3961 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4021 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4081 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4141 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4201 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4261 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4321 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     4381 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     4441 gggggggggg gggccccccc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     4501 gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     4561 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc
     4621 gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
     4681 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa
     4741 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
     4801 gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     4861 cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg
     4921 cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc
     4981 gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     5041 gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg
     5101 agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct
     5161 cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg
     5221 ggccggggag ggctcggggg aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
     5281 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     5341 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
     5401 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
     5461 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
     5521 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
     5581 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     5641 tgctggttat tgtgctgtct catcattttg gcaaagaa
//
