LOCUS       V008846                 6161 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008846
VERSION     V008846
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6161)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6161)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..36
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             43..1434
                     /gene="HNRNPK"
                     /label="Heterogeneous nuclear ribonucleoprotein K"
                     /note="Heterogeneous nuclear ribonucleoprotein K from
                     Macaca fascicularis. Accession#: Q4R4M6"
     polyA_signal    1514..1569
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1930..1946)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1954..1970)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1978..2008)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2022..2043)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2101..2297
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2303..2437
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2675..3263)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3437..4294)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4295..4399)
                     /label="AmpR promoter"
     enhancer        4434..4813
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4434..4811
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4815..5092
                     /label="chicken beta-actin promoter"
     intron          5093..6110
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggaaac tgaacagcca
       61 gaagaaacct tccctaacac tgaaaccaat ggtgaatttg gtaaacgccc tgcagaagat
      121 atggaagagg aacaagcatt taaaagatct agaaacactg atgagatggt tgaattacgc
      181 attctgcttc agagcaagaa tgctggggca gtgattggaa aaggaggcaa gaatattaag
      241 gctctccgta cagactacaa tgccagtgtt tcagtcccag acagcagtgg ccccgagcgc
      301 atattgagta tcagtgctga tattgaaaca attggagaaa ttctgaagaa aatcatccct
      361 accttggaag agggcctgca gttgccatca cccactgcaa ccagccagct cccgctcgaa
      421 tctgatgctg tggaatgctt aaattaccaa cactataaag gaagtgactt tgactgcgag
      481 ttgaggctgt tgattcatca gagtctagca ggaggaatta ttggggtcaa aggtgctaaa
      541 attaaagaac ttcgagagaa cactcaaacc accatcaagc ttttccagga atgctgtcct
      601 cattccactg acagagttgt tcttattgga ggaaaacccg atagggttgt agagtgcata
      661 aagatcatcc ttgatcttat atctgagtct cccatcaaag gacgtgcaca gccttatgat
      721 cccaattttt acgatgaaac ctatgattat ggtggtttta caatgatgtt tgatgaccgt
      781 cgcggacgcc cagtgggatt tcccatgcgg ggaagaggtg gttttgacag aatgcctcct
      841 ggtcggggtg ggcgtcccat gcctccatct agaagagatt atgatgatat gagccctcgt
      901 cgaggaccac ctccccctcc tcccggacga ggcggccggg gtggtagcag agctcggaat
      961 cttcctcttc ctccaccacc accacctaga gggggagacc tcatggccta tgacagaaga
     1021 gggagacctg gagaccgtta cgacggcatg gttggtttca gtgctgatga aacttgggac
     1081 tctgcaatag atacatggag cccatcagaa tggcagatgg cttatgaacc acagggtggc
     1141 tccggatatg attattccta tgcagggggt cgtggctcat atggtgatct tggtggacct
     1201 attattacta cacaagtaac tattcccaaa gatttggctg gatctattat tggcaaaggt
     1261 ggtcagcgga ttaaacaaat ccgtcatgag tcgggagctt cgatcaaaat tgatgagcct
     1321 ttagaaggat ccgaagatcg gatcattacc attacaggaa cacaggacca gatacagaat
     1381 gcacagtatt tgctgcagaa cagtgtgaag cagtatgcag atgttgaagg attctaagtc
     1441 gacgaagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     1501 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     1561 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     1621 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     1681 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     1741 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     1801 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     1861 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     1921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1981 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca
     2041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     2101 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2281 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     2341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2401 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     2461 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2521 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2581 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3241 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3361 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3421 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3481 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3541 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3601 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3661 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3721 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3781 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3841 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3901 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3961 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4021 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4081 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4141 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4201 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4261 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg atcgacattg
     4441 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4501 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4561 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4621 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     4681 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     4741 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     4801 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     4861 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     4921 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     4981 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     5041 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
     5101 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
     5161 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     5221 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
     5281 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     5341 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
     5401 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
     5461 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     5521 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
     5581 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
     5641 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     5701 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
     5761 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     5821 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     5881 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     5941 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     6001 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     6061 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     6121 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
//
