LOCUS       V008847                 5969 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008847
VERSION     V008847
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5969)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..39
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             46..1257
                     /gene="CASP1"
                     /label="Caspase-1"
                     /note="Caspase-1 from Homo sapiens. Accession#: P29466"
     polyA_signal    1329..1384
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1745..1761)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1769..1785)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1793..1823)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1837..1858)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1916..2112
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2118..2252
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2490..3078)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3252..4109)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4110..4214)
                     /label="AmpR promoter"
     enhancer        4245..4624
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4245..4622
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4626..4903
                     /label="chicken beta-actin promoter"
     intron          4904..5921
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 gaattcctcg agatggacta caaggacgac gatgacaagg gtaccatggc cgacaaggtc
       61 ctgaaggaga agagaaagct gtttatccgt tccatgggtg aaggtacaat aaatggctta
      121 ctggatgaat tattacagac aagggtgctg aacaaggaag agatggagaa agtaaaacgt
      181 gaaaatgcta cagttatgga taagacccga gctttgattg actccgttat tccgaaaggg
      241 gcacaggcat gccaaatttg catcacatac atttgtgaag aagacagtta cctggcaggg
      301 acgctgggac tctcagcaga tcaaacatct ggaaattacc ttaatatgca agactctcaa
      361 ggagtacttt cttcctttcc agctcctcag gcagtgcagg acaacccagc tatgcccaca
      421 tcctcaggct cagaagggaa tgtcaagctt tgctccctag aagaagctca aaggatatgg
      481 aaacaaaagt cggcagagat ttatccaata atggacaagt caagccgcac acgtcttgct
      541 ctcattatct gcaatgaaga atttgacagt attcctagaa gaactggagc tgaggttgac
      601 atcacaggca tgacaatgct gctacaaaat ctggggtaca gcgtagatgt gaaaaaaaat
      661 ctcactgctt cggacatgac tacagagctg gaggcatttg cacaccgccc agagcacaag
      721 acctctgaca gcacgttcct ggtgttcatg tctcatggta ttcgggaagg catttgtggg
      781 aagaaacact ctgagcaagt cccagatata ctacaactca atgcaatctt taacatgttg
      841 aataccaaga actgcccaag tttgaaggac aaaccgaagg tgatcatcat ccaggcctgc
      901 cgtggtgaca gccctggtgt ggtgtggttt aaagattcag taggagtttc tggaaaccta
      961 tctttaccaa ctacagaaga gtttgaggat gatgctatta agaaagccca catagagaag
     1021 gattttatcg ctttctgctc ttccacacca gataatgttt cttggagaca tcccacaatg
     1081 ggctctgttt ttattggaag actcattgaa catatgcaag aatatgcctg ttcctgtgat
     1141 gtggaggaaa ttttccgcaa ggttcgattt tcatttgagc agccagatgg tagagcgcag
     1201 atgcccacca ctgaaagagt gactttgaca agatgtttct acctcttccc aggacattaa
     1261 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     1321 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     1381 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     1441 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     1501 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     1561 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     1621 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     1681 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     1741 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1801 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat
     1861 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     1921 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2101 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2161 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2221 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     2281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2401 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2461 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2521 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2581 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2641 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2701 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2761 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2821 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2881 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2941 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3001 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3061 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3121 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3181 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3241 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3301 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3361 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3421 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3481 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3541 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3601 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3661 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3721 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3781 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3841 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3901 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3961 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     4021 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4081 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4141 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4201 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac
     4261 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     4321 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4381 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4441 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4501 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4561 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4621 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     4681 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     4741 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa
     4801 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     4861 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg
     4921 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     4981 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     5041 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     5101 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     5161 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     5221 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     5281 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     5341 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     5401 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     5461 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     5521 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     5581 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     5641 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     5701 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     5761 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     5821 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     5881 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     5941 ttattgtgct gtctcatcat tttggcaaa
//
