LOCUS       V008833                 5900 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008833
VERSION     V008833
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5900)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..27
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             28..1164
                     /gene="STING1"
                     /label="Stimulator of interferon genes protein"
                     /note="Stimulator of interferon genes protein from Homo
                     sapiens. Accession#: Q86WV6"
     polyA_signal    1244..1299
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1660..1676)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1684..1700)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1708..1738)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1752..1773)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1831..2027
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2033..2167
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2405..2993)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3167..4024)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4025..4129)
                     /label="AmpR promoter"
     enhancer        4164..4543
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4164..4541
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4545..4822
                     /label="chicken beta-actin promoter"
     intron          4823..5840
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 atggactaca aggacgacga tgacaagatg ccccactcca gcctgcatcc atccatcccg
       61 tgtcccaggg gtcacggggc ccagaaggca gccttggttc tgctgagtgc ctgcctggtg
      121 accctttggg ggctaggaga gccaccagag cacactctcc ggtacctggt gctccaccta
      181 gcctccctgc agctgggact gctgttaaac ggggtctgca gcctggctga ggagctgcac
      241 cacatccact ccaggtaccg gggcagctac tggaggactg tgcgggcctg cctgggctgc
      301 cccctccgcc gtggggccct gttgctgctg tccatctatt tctactactc cctcccaaat
      361 gcggtcggcc cgcccttcac ttggatgctt gccctcctgg gcctctcgca ggcactgaac
      421 atcctcctgg gcctcaaggg cctggcccca gctgagatct ctgcagtgtg tgaaaaaggg
      481 aatttcaacg tggcccatgg gctggcatgg tcatattaca tcggatatct gcggctgatc
      541 ctgccagagc tccaggcccg gattcgaact tacaatcagc attacaacaa cctgctacgg
      601 ggtgcagtga gccagcggct gtatattctc ctcccattgg actgtggggt gcctgataac
      661 ctgagtatgg ctgaccccaa cattcgcttc ctggataaac tgccccagca gaccgctgac
      721 cgtgctggca tcaaggatcg ggtttacagc aacagcatct atgagcttct ggagaacggg
      781 cagcgggcgg gcacctgtgt cctggagtac gccaccccct tgcagacttt gtttgccatg
      841 tcacaataca gtcaagctgg ctttagccgg gaggataggc ttgagcaggc caaactcttc
      901 tgccagacac ttgaggacat cctggcagat gcccctgagt ctcagaacaa ctgccgcctc
      961 attgcctacc aggaacctgc agatgacagc agcttctcgc tgtcccagga ggttctccgg
     1021 cacctgcggc aggaggaaaa ggaagaggtt actgtgggca gcttgaagac ctcagcggtg
     1081 cccagtacct ccacgatgtc ccaagagcct gagctcctca tcagtggaat ggaaaagccc
     1141 ctccctctcc gcacggattt ctcttgagtc gacgaagatc tttttccctc tgccaaaaat
     1201 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     1261 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     1321 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     1381 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     1441 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     1501 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     1561 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     1621 agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1681 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1741 ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1801 cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc
     1861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt
     2041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     2161 ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2221 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2281 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa
     2341 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2821 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     2881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3301 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4081 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4141 gaaaagtgcc acctgggtcg atcgacattg attattgact agttattaat agtaatcaat
     4201 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4261 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4321 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4381 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4441 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4501 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     4561 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     4621 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg
     4681 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca
     4741 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4801 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc
     4861 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     4921 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     4981 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     5041 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     5101 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     5161 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     5221 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     5281 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     5341 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     5401 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc
     5461 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     5521 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     5581 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     5641 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     5701 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     5761 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     5821 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     5881 ttggcaaaga attcctcgag
//
