LOCUS       V008854                 6079 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008854
VERSION     V008854
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6079)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6079)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             61..1263
                     /gene="Casp1"
                     /label="Caspase-1"
                     /note="Caspase-1 from Mus musculus. Accession#: P29452"
     polyA_signal    1436..1491
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1852..1868)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1876..1892)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1900..1930)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1944..1965)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2023..2219
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2225..2359
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2597..3185)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3359..4216)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4217..4321)
                     /label="AmpR promoter"
     enhancer        4352..4731
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4352..4729
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4733..5010
                     /label="chicken beta-actin promoter"
     intron          5011..6028
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgacaaga tcctgagggc aaagaggaag caatttatca actcagtgag tatagggaca
      121 ataaatggat tgttggatga acttttagag aagagagtgc tgaatcagga agaaatggat
      181 aaaataaaac ttgcaaacat tactgctatg gacaaggcac gggacctatg tgatcatgtc
      241 tctaaaaaag ggccccaggc aagccaaatc tttatcactt acatttgtaa tgaagactgc
      301 tacctggcag gaattctgga gcttcaatca gctccatcag ctgaaacatt tgttgctaca
      361 gaagaatcta aaggtggcca tccttcatcc tcagaaacaa aggaagaaca gaacaaagaa
      421 gagggtacct ttccaggact gactgggacc ctcaagtttt gccctttaga aaaagcccag
      481 aagttatgga aagaaaatcc ttcagagatt tatccaataa tgaatacaac cactcgtaca
      541 cgtcttgccc tcattatctg caacacagag tttcaacatc tttctccgag ggttggagct
      601 caagttgacc tcagagaaat gaagttgctg ctggaggatc tggggtatac cgtgaaagtg
      661 aaagaaaatc tcacagctct ggagatggtg aaagaggtga aagaatttgc tgcctgccca
      721 gagcacaaga cttctgacag tactttcctt gtattcatgt ctcatggtat ccaggaggga
      781 atatgtggga ccacatactc taatgaagtt tcagatattt taaaggttga cacaatcttt
      841 cagatgatga acactttgaa gtgcccaagc ttgaaagaca agcccaaggt gatcattatt
      901 caggcatgcc gtggagagaa acaaggagtg gtgttgttaa aagattcagt aagagactct
      961 gaagaggatt tcttaacgga tgcaattttt gaagatgatg gcattaagaa ggcccatata
     1021 gagaaagatt ttattgcttt ctgctcttca acaccagata atgtgtcttg gagacatcct
     1081 gtcaggggct cacttttcat tgagtcactc atcaaacaca tgaaagaata tgcctggtct
     1141 tgtgacttgg aggacatttt cagaaaggtt cgattttcat ttgaacaacc agaatttagg
     1201 ctacagatgc ccactgctga tagggtgacc ctgacaaaac gtttctacct cttcccggga
     1261 cattaaacgc gtgggtaccg gatccggcaa ttcactcctc aggtgcaggc tgcctatcag
     1321 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1381 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1441 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1501 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1561 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     1621 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     1681 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     1741 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     1801 cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct
     1861 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     1921 aaagtgtaaa gcctgggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1981 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
     2041 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     2101 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     2161 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     2221 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2341 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2401 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2461 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     4021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     4081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     4261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     4321 gcacatttcc ccgaaaagtg ccacctgggt cgacattgat tattgactag ttattaatag
     4381 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     4441 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4501 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     4561 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     4621 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     4681 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
     4741 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
     4801 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc
     4861 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca
     4921 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     4981 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg
     5041 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
     5101 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
     5161 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
     5221 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
     5281 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     5341 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
     5401 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     5461 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
     5521 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
     5581 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg
     5641 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     5701 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     5761 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     5821 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     5881 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     5941 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     6001 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     6061 tcatcatttt ggcaaagaa
//
