LOCUS       40924_8221        5471 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-EhRac1-DN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5471)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5471)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label=E-tag
                     /note="E-tag"
     CDS             64..636
                     /label=Rac1 (T17N)
                     /note="dominant negative mutant of human Rac family small
                     GTPase 1"
     polyA_signal    828..883
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1268..1284)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1292..1322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1336..1357)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1415..1611
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1617..1751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1989..2577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2751..3608)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3609..3713)
                     /label=AmpR promoter
     enhancer        3744..4123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3744..4121
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4125..4402
                     /label=chicken beta-actin promoter
     intron          4403..5420
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctcaggcca tcaagtgtgt ggtggtggga gacggagctg taggtaaaaa ttgcctactg
      121 atcagttaca caaccaatgc atttcctgga gaatatatcc ctactgtctt tgacaattat
      181 tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg gcttatggga tacagctgga
      241 caagaagatt atgacagatt acgcccccta tcctatccgc aaacagatgt gttcttaatt
      301 tgcttttccc ttgtgagtcc tgcatcattt gaaaatgtcc gtgcaaagtg gtatcctgag
      361 gtgcggcacc actgtcccaa cactcccatc atcctagtgg gaactaaact tgatcttagg
      421 gatgataaag acacgatcga gaaactgaag gagaagaagc tgactcccat cacctatccg
      481 cagggtctag ccatggctaa ggagattggt gctgtaaaat acctggagtg ctcggcgctc
      541 acacagcgag gcctcaagac agtgtttgac gaagcgatcc gagcagtcct ctgcccgcct
      601 cccgtgaaga agaggaagag aaaatgcctg ctgttgtgac tcgagcccgg gacattaaac
      661 gcgtgggtac cggatccggc aattcactcc tcaggtgcag gctgcctatc agaaggtggt
      721 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
      781 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
      841 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
      901 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
      961 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     1021 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     1081 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     1141 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     1201 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     1261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1321 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1381 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     1441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     1501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     1561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     1621 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1681 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1741 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1801 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1861 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca
     1921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2401 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2641 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2701 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2761 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2821 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2881 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2941 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3001 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3061 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3121 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3181 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3241 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3301 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3361 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3421 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3481 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3541 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3601 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3661 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3721 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
     3781 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     3841 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     3901 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     3961 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4021 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4081 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     4141 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     4201 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg
     4261 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca
     4321 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4381 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc
     4441 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     4501 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     4561 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     4621 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     4681 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     4741 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     4801 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     4861 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     4921 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     4981 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc
     5041 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     5101 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     5161 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     5221 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     5281 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     5341 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     5401 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     5461 ttggcaaaga a
//
