LOCUS       40924_8211        5462 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-EhCdc42-DN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5462)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5462)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label=E-tag
                     /note="E-tag"
     CDS             64..633
                     /label=Cdc42 (T17N)
                     /note="dominant negative mutant of human Rho family small
                     GTPase Cdc42"
     polyA_signal    819..874
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1235..1251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1259..1275)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1283..1313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1327..1348)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1406..1602
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1608..1742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1980..2568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2742..3599)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3600..3704)
                     /label=AmpR promoter
     enhancer        3735..4114
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3735..4112
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4116..4393
                     /label=chicken beta-actin promoter
     intron          4394..5411
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctcagacaa ttaagtgtgt tgttgtgggc gatggtgctg ttggtaaaaa ctgtctcctg
      121 atatcctaca caacaaacaa atttccatcg gaatatgtac cgactgtttt tgacaactat
      181 gcagtcacag ttatgattgg tggagaacca tatactcttg gactttttga tactgcaggg
      241 caagaggatt atgacagatt acgaccgctg agttatccac aaacagatgt atttctagtc
      301 tgtttttcag tggtctctcc atcttcattt gaaaacgtga aagaaaagtg ggtgcctgag
      361 ataactcacc actgtccaaa gactcctttc ttgcttgttg ggactcaaat tgatctcaga
      421 gatgacccct ctactattga gaaacttgcc aagaacaaac agaagcctat cactccagag
      481 actgctgaaa agctggcccg tgacctgaag gctgtcaagt atgtggagtg ttctgcactt
      541 acacagaaag gcctaaagaa tgtatttgac gaagcaatat tggctgccct ggagcctcca
      601 gaaccgaaga agagccgcag gtgtgtggtg ctatgacccg ggacattaaa cgcgtgggta
      661 ccggatccgg caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
      721 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
      781 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
      841 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
      901 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
      961 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     1021 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     1081 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     1141 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     1201 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1321 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1381 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
     1441 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     1501 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     1561 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
     1621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     1741 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1801 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1861 agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac
     1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3721 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     3781 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     3841 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3901 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3961 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4021 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     4081 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     4141 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     4201 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg
     4261 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc
     4321 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     4381 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc
     4441 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     4501 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     4561 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     4621 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     4681 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     4741 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     4801 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     4861 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     4921 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     4981 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc
     5041 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     5101 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     5161 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     5221 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     5281 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     5341 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     5401 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     5461 aa
//
