LOCUS       V008849                 5855 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008849
VERSION     V008849
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5855)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1026
                     /gene="yopT"
                     /label="Cysteine protease YopT"
                     /note="Cysteine protease YopT from Yersinia
                     pseudotuberculosis serotype I (strain IP32953). Accession#:
                     Q93RN4"
     polyA_signal    1212..1267
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1628..1644)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1652..1668)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1676..1706)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1720..1741)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1799..1995
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2001..2135
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2373..2961)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3135..3992)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3993..4097)
                     /label="AmpR promoter"
     enhancer        4128..4507
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4128..4505
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4509..4786
                     /label="chicken beta-actin promoter"
     intron          4787..5804
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgacagta ttcacggaca ctaccatatc caactatcga attattctgc cggtgaaaac
      121 cttcaatcag ctacactcac cgaaggggtg attggcgcac accgagtgaa agtggaaaca
      181 gcactgtcac actcaaaccg gcagaaaaag ttatcagcta ccataaaaca taaccagtca
      241 agccgttcta tgctggatag aaagttgacc agcgacggca aagctaacca acgaagcagc
      301 tttaccttca gtatgattat gtatcgcatg atacattttg tactcagtac tcgtgtgccc
      361 gcggtgagag agtctgttgc aaattacgga ggtaacatca atttcaagtt tgctcagacc
      421 aaaggggctt ttcttcatca aataataaaa cattcagaca ccgctagcgg tgtcagtgag
      481 gctttatgtg cacattggat ttggagtcat gcacagggcc aaagcttatt tgaccagctc
      541 tatgttggcg ggcgtaaagg gaaattccag atcgatacac tttactcaat taaacagttg
      601 caaatagatg gttgtaaagc agacgttgat caagatgagg taacactaga ttggttcaag
      661 aaaaagggca tatcagaacg tatgattgaa cggcattgct tactgcgtcc agttgatgtt
      721 actggtacga cggaatcaga agggccggat caattattaa acgctatcct tgatactcat
      781 gggataggtt acggttataa aaaaatatat ctctcaggcc aaatgtcagc ccacgccata
      841 gcggcgtatg tcaacgaaaa gagtggtgtt actttcttcg atcccaattt cggtgaattc
      901 cacttttctg ataaggaaaa gttccgcaaa tggtttacta actcattctg ggaaaattct
      961 atgtatcatt atcctctggg ggtggggcag cgttttagcg tgttaacatt tgactccaag
     1021 gaggtttgac ccgggacatt aaacgcgtgg gtaccggatc cggcaattca ctcctcaggt
     1081 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     1141 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     1201 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     1261 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     1321 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     1381 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     1441 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     1501 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     1561 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     1621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1681 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt
     1741 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     1801 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1861 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1921 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1981 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     2041 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2101 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     2161 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2221 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2281 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3721 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt
     4141 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     4201 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     4261 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     4321 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     4381 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     4441 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     4501 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     4561 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     4621 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     4681 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     4741 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg
     4801 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     4861 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     4921 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     4981 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     5041 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     5101 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     5161 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     5221 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     5281 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     5341 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     5401 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     5461 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     5521 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     5581 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     5641 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     5701 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     5761 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     5821 tggttattgt gctgtctcat cattttggca aagaa
//
