LOCUS       V008850                 6293 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008850
VERSION     V008850
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6293)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6293)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1464
                     /gene="yopH"
                     /label="Tyrosine-protein phosphatase YopH"
                     /note="Tyrosine-protein phosphatase YopH from Yersinia
                     enterocolitica. Accession#: P15273"
     polyA_signal    1650..1705
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2066..2082)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2090..2106)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2114..2144)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2158..2179)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2237..2433
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2439..2573
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2811..3399)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3573..4430)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4431..4535)
                     /label="AmpR promoter"
     enhancer        4566..4945
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4566..4943
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4947..5224
                     /label="chicken beta-actin promoter"
     intron          5225..6242
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctaacttat cattaagcga tcttcatcgt caggtatctc gattggtgca gcaagagagc
      121 ggtgattgta ccgggaaatt aagaggtaac gttgctgcca ataaagaaac tacctttcaa
      181 ggtttgacca tagccagtgg tgccagagag tcagaaaaag tatttgctca aactgtacta
      241 agccacgtag caaatattgt tctaactcaa gaagataccg ctaagctatt gcaaagcacg
      301 gtaaagcata atttgaataa ttatgaatta agaagtgtcg gcaatggtaa tagtgtactt
      361 gtcagtttac gtagtgacca aatgacacta caagacgcca aagtgctgtt ggaggctgca
      421 ttgcgacaag agtcgggagc gagggggcat gtatcatctc attcacattc agtccttcac
      481 gcaccgggaa ccccggtgcg tgaaggactg cgttcacatc tagaccccag aacaccaccg
      541 ttgccaccgc gtgaacgacc acacacttct ggccatcacg gggctggcga agccagagcc
      601 accgcaccaa gcactgtttc tccttatggc ccagaagcgc gcgcagaact cagcagccgc
      661 ctcaccacat tgcgcaatac gctggcgcca gcaacgaatg atccgcgtta cttacaagcc
      721 tgcggcggtg aaaagctaaa ccgatttaga gatattcaat gctgtcggca aaccgcagta
      781 cgcgccgatc ttaatgccaa ttacatccag gtcggtaaca ctcgtaccat agcgtgccag
      841 tatccgctac aatctcaact tgaaagccat ttccgtatgc tggcagaaaa ccgaacgcca
      901 gtgttggctg ttttagcgtc cagttctgag atagccaatc aaagattcgg tatgccagat
      961 tatttccgcc agagtggtac ctatggcagt atcactgtag agtctaaaat gactcagcaa
     1021 gttggtctcg gtgacgggat tatggcagat atgtatactt taacgattcg tgaagcgggt
     1081 caaaaaacaa tttctgttcc tgtggttcat gttggcaatt ggcccgatca gaccgcagtc
     1141 agctctgaag ttaccaaggc actcgcttca ctggtagatc aaacagcaga aacaaaacgc
     1201 aatatgtatg aaagcaaagg aagttcagcg gtagcagatg actccaaatt acggccggta
     1261 atacattgcc gtgcgggtgt tggccgtact gcgcaactga ttggcgcaat gtgcatgaat
     1321 gatagtcgta atagtcagtt aagcgtagaa gatatggtca gccaaatgcg agtacaaaga
     1381 aatggtatta tggtacaaaa agatgagcaa cttgatgttc tgattaagtt ggctgaagga
     1441 caagggcgac cattattaaa tagctaaccc gggacattaa acgcgtgggt accggatccg
     1501 gcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     1561 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     1621 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     1681 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     1741 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     1801 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     1861 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     1921 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     1981 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     2041 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2101 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg
     2161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2221 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2281 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2341 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2401 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     2461 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     2581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2701 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca
     2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4561 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     4621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4921 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     4981 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     5041 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     5101 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     5161 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     5221 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     5281 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     5341 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     5401 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     5461 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     5521 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     5581 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     5641 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     5701 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     5761 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     5821 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     5881 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     5941 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     6001 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     6061 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     6121 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     6181 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     6241 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa
//
