LOCUS       V008851                 5546 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008851
VERSION     V008851
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5546)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5546)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..717
                     /gene="yopE"
                     /label="Outer membrane virulence protein YopE"
                     /note="Outer membrane virulence protein YopE from Yersinia
                     pestis. Accession#: P31493"
     polyA_signal    903..958
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1319..1335)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1343..1359)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1367..1397)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1411..1432)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1490..1686
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1692..1826
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2064..2652)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2826..3683)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3684..3788)
                     /label="AmpR promoter"
     enhancer        3819..4198
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3819..4196
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4200..4477
                     /label="chicken beta-actin promoter"
     intron          4478..5495
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctaaaatat catcatttat ttctacatca ctgcccctgc cggcatcagt gtcaggatct
      121 agcagcgtag gagaaatgtc tgggcgctca gtctcacagc aaaaaagtga tcaatatgca
      181 aacaatctgg ccgggcgcac tgaaagccct cagggttcca gcttagccag ccgtatcatt
      241 gagaggttat catcaatggc ccactctgtg attggattta tccaacgcat gttctcggag
      301 gggagccata aaccggtggt gacaccagca ctcacgcctg cacaaatgcc aagccctacg
      361 tctttcagtg atagtatcaa gcaacttgct gctgagacgc tgccaaaata catgcagcag
      421 ttgagtagct tggatgcaga gacgctgcag aaaaatcatg accagttcgc cacgggcagc
      481 ggccctcttc gtggcagtat cactcaatgc caagggctga tgcagttttg tggtggggaa
      541 ttgcaagctg aggccagtgc cattttaaac acgcctgttt gtggtattcc cttctcgcag
      601 tggggaactg ttggtggggc ggccagcgcg tacgtcgcca gtggcgttga tctaacgcag
      661 gcagcaaatg agatcaaagg gctggggcaa cagatgcagc aattactgtc attgatgtga
      721 cccgggacat taaacgcgtg ggtaccggat ccggcaattc actcctcagg tgcaggctgc
      781 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
      841 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
      901 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
      961 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     1021 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     1081 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     1141 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1201 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1261 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     1321 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1381 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
     1441 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     1501 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1561 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1621 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1681 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1741 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1801 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     1861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1981 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2041 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2101 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2161 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2221 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2281 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2401 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2461 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2521 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2581 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2641 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2701 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2761 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2821 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2881 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2941 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3001 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3061 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3121 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3181 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3241 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3301 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3361 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3421 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3481 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3541 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3601 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3661 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3721 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3781 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
     3841 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3901 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3961 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4021 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4081 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4141 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4201 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4261 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     4321 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
     4381 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     4441 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
     4501 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     4561 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     4621 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     4681 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     4741 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     4801 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     4861 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     4921 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     4981 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     5041 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     5101 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     5161 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     5221 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     5281 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     5341 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     5401 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     5461 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     5521 tgctgtctca tcattttggc aaagaa
//
