LOCUS       V038213                 6586 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038213
VERSION     V038213
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        4..383
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        385..662
                     /label="chicken β-actin promoter"
     intron          663..1680
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
                     /label="chimeric intron"
     CDS             1738..1764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             1771..3573
                     /label="ESCO2(NM_001017420)"
                     /note="ESCO2(NM_001017420)"
                     /gene="ESCO2"
     polyA_signal    3673..3728
                     /note="rabbit&nbsp;β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
                     /label="β-globin poly(A) signal"
     primer_bind     complement(4089..4105)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4113..4129
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4137..4167)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4182..4203
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        4262..4457
                     /note="SV40 early promoter"
                     /label="SV40 promoter"
     polyA_signal    4463..4597
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4835..5423)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5594..6454)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6455..6559)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaagc caccatgtac
     1741 ccatacgatg ttccagatta cgctgaattc atggcagctc ttactccaag gaagaggaag
     1801 caggattctt tgaagtgtga cagcctttta cacttcactg aaaatctgtt tccatcacct
     1861 aataaaaagc actgttttta tcaaaacagt gataaaaatg aagaaaacct gcattgctct
     1921 caacaagagc attttgtttt aagtgcgctc aaaacaactg aaataaatag actgccatca
     1981 gcaaatcaag gctcaccatt taaatctgcg ctctccactg tatcttttta caaccaaaat
     2041 aagtggtacc tcaatccact ggagagaaag ctgataaaag agagtagatc tacttgtcta
     2101 aaaactaatg atgaagataa atcttttccc attgtgacag aaaaaatgca aggaaaacca
     2161 gtctgctcca agaagaacaa caaaaaacca cagaagagtt taactgctaa gtatcaacca
     2221 aagtatagac acatcaagcc tgtatcaagg aattctagaa attccaagca aaatcgagtg
     2281 atctataagc caattgtgga gaaggaaaat aattgtcatt cagctgaaaa taattccaat
     2341 gctcctcggg ttctgagcca aaaaataaaa ccacaagtta cactccaggg tggagcagca
     2401 ttttttgtta gaaaaaaatc ttctcttaga aaatcgtccc tggaaaatga gccgtcactg
     2461 ggacgcaccc aaaagagtaa atcagaagtc attgaagatt ctgatgtaga gactgtcagt
     2521 gaaaaaaaaa cttttgcgac aaggcaagtg ccaaagtgct tggtcctaga agagaaattg
     2581 aaaattggac tactgagtgc aagcagtaaa aataaagaga aattaataaa ggattcatca
     2641 gatgacagag tttcttcaaa ggaacataaa gttgataaaa atgaggcttt ttcttcagag
     2701 gattctcttg gtgagaataa gacaatttct cctaagtcca ctgtctatcc aatcttcagt
     2761 gcatcttcag tcaattcaaa aagatcttta ggtgaagaac agttttctgt gggatctgtc
     2821 aacttcatga aacagaccaa tatccagaaa aatactaata ccagagatac aagtaaaaaa
     2881 acaaaagacc agctcatcat cgacgctggt cagaaacatt ttggggctac tgtgtgcaag
     2941 tcttgtggta tgatatatac tgcttccaac cctgaagatg aaatgcagca tgtacagcat
     3001 caccacaggt ttctggaagg aatcaaatat gtgggttgga agaaagaacg tgtagtagca
     3061 gagttttggg atgggaaaat cgtgttggtt ctgccacatg atccaagctt tgctatcaaa
     3121 aaggtagaag atgtccaaga acttgttgat aatgaattgg gcttccagca agttgttcct
     3181 aaatgtccaa acaaaataaa aacttttctt tttatatctg atgaaaagag agtagttggg
     3241 tgtttaattg cagaacccat caaacaggca tttcgtgtcc tgtctgaacc aattggtcca
     3301 gaatccccaa gctctacgga atgtcctagg gcttggcaat gttcagatgt accagaacct
     3361 gcagtctgtg ggataagtag aatctgggtt ttcagactga agagaagaaa gcgcattgca
     3421 agacgactgg ttgataccct caggaattgc ttcatgtttg gctgttttct cagcactgat
     3481 gaaatagcat tttctgaccc aacaccagat ggcaagttat ttgcaaccaa gtactgcaac
     3541 acccctaatt tcctcgtata taattttaat agttaactcg agctagcaga tcttaattaa
     3601 ttaaggatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3661 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3721 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3781 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3841 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     3901 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3961 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     4021 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     4081 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4141 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4201 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     4261 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4321 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4381 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4441 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     4501 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4561 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     4621 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4681 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4741 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4801 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4861 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4921 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4981 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5041 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5101 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5161 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5221 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5281 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5341 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5401 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5461 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5521 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5581 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5641 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5701 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5761 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5821 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5881 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5941 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6001 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6061 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6121 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6181 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6241 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6301 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6361 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6421 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6481 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6541 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgg
//
