LOCUS       V008862                 6521 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008862
VERSION     V008862
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6521)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6521)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1692
                     /gene="sptP"
                     /label="Secreted effector protein SptP"
                     /note="Secreted effector protein SptP from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P74873"
     polyA_signal    1878..1933
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2294..2310)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2318..2334)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2342..2372)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2386..2407)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2465..2661
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2667..2801
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3039..3627)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3801..4658)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4659..4763)
                     /label="AmpR promoter"
     enhancer        4794..5173
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4794..5171
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5175..5452
                     /label="chicken beta-actin promoter"
     intron          5453..6470
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctatgctaa agtatgagga gagaaaattg aataatttaa cgttgtcttc gttttcaaaa
      121 gttggtgtgt cgaatgatgc ccgactttat attgctaagg aaaatactga taaggcatat
      181 gttgcgcctg aaaaattttc gtcaaaagta ttaacctggc ttggaaaaat gccgttattt
      241 aaaaacactg aagtggtgca aaaacatacg gaaaatatca gagtacagga ccaaaagatt
      301 ttacagacat ttctccatgc actaacggaa aaatatgggg aaacagcggt taatgacgca
      361 ctgttaatgt cccgtataaa tatgaacaaa ccccttaccc aacgtttagc agtgcagatc
      421 acggagtgtg taaaagctgc tgacgaaggg tttataaacc ttattaagag caaggataat
      481 gttggtgtca ggaatgccgc tttagtcata aaaggcggcg atacaaaagt ggcagaaaaa
      541 aataacgatg ttggagcaga aagtaagcaa cctttactcg atatcgcgct aaagggactt
      601 aaaagaacat taccgcaact ggaacaaatg gatggaaata gcctgcgaga aaacttccag
      661 gagatggctt caggtaacgg cccgctgcgt tcattaatga cgaatttaca gaatttaaat
      721 aaaattccag aggctaaaca acttaatgat tatgttacaa cgttaacaaa tattcaggtc
      781 ggtgtagccc ggttctcaca atggggaacc tgtggaggag aggttgagag gtgggttgat
      841 aaagcctcta ctcatgaatt aacccaggct gtaaaaaaaa ttcacgttat tgctaaagaa
      901 ctgaaaaatg ttacagctga acttgagaaa atagaagccg gcgcgccaat gccacagacg
      961 atgagcggac cgacactggg cctggcacga tttgcggtca gcagcattcc aattaatcag
     1021 caaacccagg tgaaactgag cgatggaatg cctgtgccag tgaatacgtt aacttttgac
     1081 ggtaagcctg tggcattagc cggttcgtac ccaaaaaata cgccggatgc gctggaagcg
     1141 catatgaaaa tgcttcttga aaaagaatgc tcgtgcctgg tggtgttaac gtcggaagat
     1201 cagatgcagg caaaacaatt accaccctat ttcagaggga gctacacctt tggcgaggtg
     1261 cataccaaca gccaaaaagt gagctcagcc agtcagggag aagcgataga ccaatacaat
     1321 atgcaactgt cctgcgggga aaagcggtat acaatcccgg tattgcatgt gaaaaattgg
     1381 ccagatcacc agccgttacc gtctacggat cagttagaat acctggcgga tagggtgaaa
     1441 aatagtaacc aaaatggcgc gccggggcgt agttcatcag ataagcattt accgatgatt
     1501 cattgtctgg gcggagtggg aagaaccgga acgatggcgg cggcccttgt acttaaggat
     1561 aatcctcata gtaatctgga gcaggtacgt gcagatttcc gggattcacg gaacaataga
     1621 atgctggaag acgcctcaca gtttgtacaa ctaaaagcaa tgcaagccca gttgcttatg
     1681 acgacggcaa gctgacccgg gacattaaac gcgtgggtac cggatccggc aattcactcc
     1741 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa
     1801 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga
     1861 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     1921 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga
     1981 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg
     2041 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag
     2101 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt
     2161 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact
     2221 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct
     2281 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2341 acaacatacg agccggaagc ataaagtgta aagcctgggt gcctaatgag tgagctaact
     2401 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg
     2461 gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2521 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2581 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2641 ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat ggttacaaat
     2701 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2761 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt aatgaatcgg
     2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2941 acggttatcc acagaatcag ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3001 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3061 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3121 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3181 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3241 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3301 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3361 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3421 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3481 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3541 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3601 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3661 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3721 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3781 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3841 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3901 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3961 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4021 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4081 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4141 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4201 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4261 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4321 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4381 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4441 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4501 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4561 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4621 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4681 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4741 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     4801 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     4861 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     4921 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     4981 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5041 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     5101 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     5161 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     5221 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     5281 gggggggggg ggggggcccc ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc
     5341 gaggcggaaa ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat
     5401 ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt
     5461 tgcgcgctgc cttccccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct
     5521 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     5581 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga
     5641 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     5701 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg
     5761 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc
     5821 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg
     5881 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc
     5941 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg
     6001 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc
     6061 tcgggccggg gagggctcgg gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc
     6121 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     6181 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     6241 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     6301 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     6361 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     6421 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     6481 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga a
//
