LOCUS       V008864                 5612 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008864
VERSION     V008864
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5612)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5612)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..783
                     /gene="sopE"
                     /label="Guanine nucleotide exchange factor SopE"
                     /note="Guanine nucleotide exchange factor SopE from
                     Salmonella typhimurium. Accession#: O52623"
     polyA_signal    969..1024
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1385..1401)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1409..1425)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1433..1463)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1477..1498)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1556..1752
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1758..1892
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2130..2718)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2892..3749)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3750..3854)
                     /label="AmpR promoter"
     enhancer        3885..4264
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3885..4262
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4266..4543
                     /label="chicken beta-actin promoter"
     intron          4544..5561
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgtgacaa aaataacttt atctccccag aattttagaa tccaaaaaca ggaaaccaca
      121 ctactaaaag aaaaatcaac cgagaaaaat tctttagcaa aaagtattct cgcagtaaaa
      181 aatcacttca tcgaattaag gtcaaaatta tcggaacgtt ttatttcgca taagaacact
      241 gagtcttctg caacacactt tcaccgagga agcgcatctg agggccgggc agtgttgaca
      301 aataaagtcg ttaaagattt tatgcttcaa acgctcaatg atatagatat tagaggtagt
      361 gcgagtaaag accccgcata cgccagccag acccgtgaag ctatactatc ggcagtttac
      421 agcaagaata aagatcagtg ttgtaatttg ctcatcagca aagggatcaa catagcgcct
      481 tttcttcagg aaattggcga agcagcgaaa aatgcaggtc tgcccggaac aaccaaaaat
      541 gacgttttta cgccaagcgg cgcaggggcc aatcctttta taactccgtt gatttcatca
      601 gcaaacagta agtatccacg tatgtttatc aatcaacatc agcaggcatc ctttaaaatc
      661 tatgcggaga agatcattat gacagaagtt gcaccactgt ttaatgagtg tgctatgccg
      721 actccacagc aattccaact gatactagaa aacattgcta ataaatatat acaaaacact
      781 ccctaacccg ggacattaaa cgcgtgggta ccggatccgg caattcactc ctcaggtgca
      841 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
      901 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
      961 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     1021 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     1081 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     1141 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     1201 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     1261 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     1321 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     1381 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1441 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat
     1501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     1561 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1621 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1681 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1741 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1801 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1861 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     1921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2041 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac
     3901 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3961 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4021 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4081 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4141 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4201 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4261 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     4321 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     4381 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa
     4441 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     4501 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg
     4561 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     4621 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     4681 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     4741 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     4801 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     4861 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     4921 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     4981 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     5041 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     5101 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     5161 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     5221 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     5281 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     5341 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     5401 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     5461 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     5521 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     5581 ttattgtgct gtctcatcat tttggcaaag aa
//
