LOCUS       V008866                 6668 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008866
VERSION     V008866
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6668)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6668)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1839
                     /gene="sctE1"
                     /label="SPI-1 type 3 secretion system translocon protein
                     SctE"
                     /note="SPI-1 type 3 secretion system translocon protein
                     SctE from Salmonella typhimurium (strain LT2 / SGSC1412 /
                     ATCC 700720). Accession#: Q56019"
     polyA_signal    2025..2080
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2441..2457)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2465..2481)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2489..2519)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2533..2554)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2612..2808
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2814..2948
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3186..3774)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3948..4805)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4806..4910)
                     /label="AmpR promoter"
     enhancer        4941..5320
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4941..5318
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5322..5599
                     /label="chicken beta-actin promoter"
     intron          5600..6617
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgtaaatg acgcaagtag cattagccgt agcggatata cccaaaatcc gcgcctcgct
      121 gaggcggctt ttgaaggcgt tcgtaagaac acggactttt taaaagcggc ggataaagct
      181 tttaaagatg tggtggcaac gaaagcgggc gaccttaaag ccggaacaaa gtccggcgag
      241 agcgctatta atacggtggg tctaaagccg cctacggacg ccgcccggga aaaactctcc
      301 agcgaagggc aattgacatt actgcttggc aagttaatga ccctactggg cgatgtttcg
      361 ctgtctcaac tggagtctcg tctggcggta tggcaggcga tgattgagtc acaaaaagag
      421 atggggattc aggtatcgaa agaattccag acggctctgg gagaggctca ggaggcgacg
      481 gatctctatg aagccagtat caaaaagacg gataccgcca agagtgttta tgacgctgcg
      541 accaaaaaac tgacgcaggc gcaaaataaa ttgcaatcgc tggacccggc tgaccccggc
      601 tatgcacaag ctgaagccgc ggtagaacag gccggaaaag aagcgacaga ggcgaaagag
      661 gccttagata aggccacgga tgcgacggtt aaagcaggca cagacgccaa agcgaaagcc
      721 gagaaagcgg ataacattct gaccaaattc cagggaacgg ctaatgccgc ctctcagaat
      781 caggtttccc agggtgagca ggataatctg tcaaatgtcg cccgcctcac tatgctcatg
      841 gccatgttta ttgagattgt gggcaaaaat acggaagaaa gcctgcaaaa cgatcttgcg
      901 cttttcaacg ccttgcagga agggcgtcag gcggagatgg aaaagaaatc ggctgaattc
      961 caggaagaga cgcgcaaagc cgaggaaacg aaccgcatta tgggatgtat cgggaaagtc
     1021 ctcggcgcgc tgctaaccat tgtcagcgtt gtggccgctg tttttaccgg tggggcgagt
     1081 ctggcgctgg ctgcggtggg acttgcggta atggtggccg atgaaattgt gaaggcggcg
     1141 acgggagtgt cgtttattca gcaggcgcta aacccgatta tggagcatgt gctgaagccg
     1201 ttaatggagc tgattggcaa ggcgattacc aaagcgctgg aaggattagg cgtcgataag
     1261 aaaacggcag agatggccgg cagcattgtt ggtgcgattg tcgccgctat tgccatggtg
     1321 gcggtcattg tggtggtcgc agttgtcggg aaaggcgcgg cggcgaaact gggtaacgcg
     1381 ctgagcaaaa tgatgggcga aacgattaag aagttggtgc ctaacgtgct gaaacagttg
     1441 gcgcaaaacg gcagcaaact ctttacccag gggatgcaac gtattactag cggtctgggt
     1501 aatgtgggta gcaagatggg cctgcaaacg aatgccttaa gtaaagagct ggtaggtaat
     1561 accctaaata aagtggcgtt gggcatggaa gtcacgaata ccgcagccca gtcagccggt
     1621 ggtgttgccg agggcgtatt tattaaaaat gccagcgagg cgcttgctga ttttatgctc
     1681 gcccgttttg ccatggatca gattcagcag tggcttaaac aatccgtaga aatatttggt
     1741 gaaaaccaga aggtaacggc ggaactgcaa aaagccatgt cttctgcggt acagcaaaat
     1801 gcggatgctt cgcgttttat tctgcgccag agtcgcgcat aacccgggac attaaacgcg
     1861 tgggtaccgg atccggcaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     1921 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     1981 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     2041 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     2101 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     2161 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     2221 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     2281 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     2341 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2401 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2461 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2521 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2581 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     2641 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2701 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2761 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     2821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2941 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3001 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3061 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     3121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4921 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     4981 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5041 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5101 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5161 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     5221 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     5281 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     5341 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     5401 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     5461 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     5521 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     5581 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     5641 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     5701 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     5761 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     5821 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     5881 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     5941 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     6001 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     6061 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     6121 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     6181 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     6241 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     6301 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     6361 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     6421 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     6481 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     6541 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     6601 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     6661 gcaaagaa
//
