LOCUS       V008868                 6395 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008868
VERSION     V008868
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6395)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6395)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1680
                     /gene="Ripk2"
                     /label="Receptor-interacting serine/threonine-protein
                     kinase 2"
                     /note="Receptor-interacting serine/threonine-protein kinase
                     2 from Mus musculus. Accession#: P58801"
     polyA_signal    1752..1807
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2168..2184)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2192..2208)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2216..2246)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2260..2281)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2339..2535
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2541..2675
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2913..3501)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3675..4532)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4533..4637)
                     /label="AmpR promoter"
     enhancer        4668..5047
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4668..5045
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5049..5326
                     /label="chicken beta-actin promoter"
     intron          5327..6344
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctatgaacg gggacgccat ctgcagcgcg ctacccccca tcccgtacca caagctcgcc
      121 gacctgcact acctcagccg gggcgcctct ggcacggtct cgtcggcacg acacgcagac
      181 tggcgcgtgc gggtggcagt gaagcaccta cacatccaca cgccgctgct cgacagtgaa
      241 agaaatgata tcttacgaga agccgaaata ttacataaag caagatttag ttacattctt
      301 ccaattttgg gaatttgcaa tgagcctgaa tttttgggca tagttactga atacatgcca
      361 aatggatcat taaatgagct tctacacaga aaaactgaat atcctgatat tgcatggcca
      421 ttgagattcc gcatcctgca tgaaattgct cttggtgtaa attatctgca caatatgaac
      481 ccccctctgc ttcatcatga cctaaagact cagaatatct tgttggataa tgaatttcat
      541 gttaagattg cagattttgg tttatcaaaa tggcgcatga tgtcactctc tcaatcacga
      601 agttacaaat ctgcacccga aggaggaaca atcatctata tgccacctga gaactatgag
      661 ccaggacaga aatcaagggc cagtgtgaag catgatatat acagctatgc agttatcatg
      721 tgggaagtgt tatccagaaa acagcctttt gaagaggtca ccaatccttt gcagatcatg
      781 tacagtgtgt cccagggaca tcgacctgac accagtgagg agaatttgcc atttgatata
      841 cctcatcgag gtctcatgat ctctctaata cagagtggat gggcgcaaaa cccagatgaa
      901 agaccatcct ttttgaaatg cttaatagaa cttgaaccag ttctgagaac atttgaagac
      961 ataactttcc ttgaggctgt tattcagcta aagaaagcaa agatacagag ttcctcaagt
     1021 actattcact tatgtgataa gaaaatggat ttatcgctaa acatacctgc aaatcatccc
     1081 ccacaggagg aatcgtgtgg atcctctctg ctctctagaa acactggttc tcctggaccc
     1141 tccagatccc tatcagctcc tcaagacaag ggatttttat ctggagcacc tcaagactgc
     1201 tcttcgttga aggcacacca ctgtcctgga aaccacagct gggatggtat cgtttctgta
     1261 ccaccagggg cagccttctg tgatcgcagg gcctcctcgt gttccttggc tgtaataagt
     1321 ccattcttgg ttgagaaagg ttcagagcgc ccaccaattg gtatagccca acagtggatc
     1381 cagagtaaga gggaagccat tgtgagccag atgactgaag cctgccttaa ccaatcgctg
     1441 gacgcccttc tgtctcgtga cctgatcatg aaggaggact atgaactcat tagcaccaaa
     1501 cctacaagga cctcaaaagt cagacagctg ctagatacgt ctgacatcca aggagaagag
     1561 tttgccaaag ttgtagtgca gaagttgaaa gataacaagc agctggggct gcagccttac
     1621 ccagaagtac ctgtgctttc caaagcaccg ccttcaaatt tccctcagaa taaaagcttg
     1681 tgaagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     1741 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     1801 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     1861 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     1921 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     1981 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     2041 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     2101 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     2161 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2221 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt
     2281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg
     2341 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2401 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2461 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     2521 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca
     2581 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     2641 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg
     2701 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2821 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3001 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3721 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4081 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt
     4681 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     4741 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     4801 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     4861 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     4921 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     4981 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     5041 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     5101 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     5161 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
     5221 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     5281 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg
     5341 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     5401 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     5461 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     5521 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     5581 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     5641 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     5701 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     5761 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     5821 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     5881 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     5941 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     6001 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     6061 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     6121 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     6181 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     6241 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     6301 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     6361 tggttattgt gctgtctcat cattttggca aagaa
//
