LOCUS       V008869                 5666 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008869
VERSION     V008869
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5666)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5666)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..951
                     /gene="Myd88"
                     /label="Myeloid differentiation primary response protein
                     MyD88"
                     /note="Myeloid differentiation primary response protein
                     MyD88 from Mus musculus. Accession#: P22366"
     polyA_signal    1023..1078
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1439..1455)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1463..1479)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1487..1517)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1531..1552)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1610..1806
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1812..1946
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2184..2772)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2946..3803)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3804..3908)
                     /label="AmpR promoter"
     enhancer        3939..4318
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3939..4316
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4320..4597
                     /label="chicken beta-actin promoter"
     intron          4598..5615
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gcgatgtctg cgggagaccc ccgcgtggga tccgggtccc tggactcctt catgttctcc
      121 atacccttgg tcgcgcttaa cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct
      181 cggacgcccg tggcggccga ctggaccttg ctcccggagg agatgggctt cgagtacttg
      241 gagatccgag agctggaaac gcgccctgac cccactcgca gtttgttgga tgcctggcag
      301 gggcgctctg gcgcgtctgt cggcaggctg ctagagctgc tggccttgtt agaccgtgag
      361 gatatactga aggagctgaa gtcgcgcatc gaggaggact gccagaaata cttaggtaag
      421 cagcagaacc aggagtccga gaagccttta caggtggcca gagtggaaag cagtgtccca
      481 caaacaaagg aactgggagg catcaccacc cttgatgacc ccctaggaca aacgccggaa
      541 cttttcgatg cctttatctg ctactgcccc aacgatatcg agtttgtgca ggagatgatc
      601 cggcaactag aacagacaga ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg
      661 ccgggcacct gtgtctggtc cattgccagc gagctaattg agaaaaggtg tcgccgcatg
      721 gtggtggttg tttctgacga ttatctacag agcaaggaat gtgacttcca gaccaagttt
      781 gcactcagcc tgtctccagg tgtccaacag aagcgactga ttcctattaa atacaaggcg
      841 atgaagaagg actttcccag tatcctgcgg ttcatcacta tatgcgacta taccaaccct
      901 tgcaccaagt cctggttctg gacccgcctt gccaaggctt tgtccctgcc ctgaagatct
      961 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     1021 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     1081 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     1141 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     1201 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     1261 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1321 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1381 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     1441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1501 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
     1561 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     1621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1801 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1921 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     1981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2101 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2161 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2221 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2281 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2341 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2401 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2461 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2521 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2581 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2641 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2701 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2761 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2821 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2881 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2941 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3001 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3061 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3121 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3181 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3241 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3301 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3361 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3421 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3481 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3541 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3601 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3661 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3721 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3781 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3841 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3901 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
     3961 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     4021 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     4081 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     4141 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     4201 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4261 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4321 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4381 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     4441 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
     4501 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     4561 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
     4621 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     4681 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     4741 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     4801 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     4861 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     4921 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     4981 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     5041 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     5101 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     5161 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     5221 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     5281 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     5341 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     5401 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     5461 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     5521 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     5581 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     5641 tgctgtctca tcattttggc aaagaa
//
