LOCUS       V008870                 5339 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008870
VERSION     V008870
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5339)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5339)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..51
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..624
                     /gene="Bnip3"
                     /label="BCL2/adenovirus E1B 19 kDa protein-interacting
                     protein 3"
                     /note="BCL2/adenovirus E1B 19 kDa protein-interacting
                     protein 3 from Mus musculus. Accession#: O55003"
     polyA_signal    696..751
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1112..1128)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1136..1152)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1160..1190)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1204..1225)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1283..1479
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1485..1619
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1857..2445)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2619..3476)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3477..3581)
                     /label="AmpR promoter"
     enhancer        3612..3991
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3612..3989
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        3993..4270
                     /label="chicken beta-actin promoter"
     intron          4271..5288
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt
       61 accatgtcgc agagcgggga ggagaacctg cagggctcct gggtagaact gcacttcagc
      121 aatggcaatg ggagcagcgt tccagcctcc gtctctattt ataatggtga catggaaaaa
      181 atactgttgg atgcccagca tgaatctgga cgaagtagct ccaagagttc tcactgtgac
      241 agcccacctc gctcccagac accacaagat accaacagag ctgaaataga cagccacagc
      301 tttggcgaga aaaacagcac tctgtctgag gaagattata ttgagagaag aagagaagtt
      361 gaaagtatcc tgaagaaaaa ctcagattgg atatgggatt ggtcaagtcg accagaaaat
      421 attcccccca aggagttcct ttttaaacac ccgaagcgca cagctactct cagcatgaga
      481 aacacaagcg ttatgaagaa agggggaatt ttctcagcag actttctgaa ggttttcctt
      541 ccatctctgt tactgtctca tctgctggcc attggcttgg ggatctacat tggaaggcgt
      601 ctgacaactt ccactagcac cttctgaaga tctttttccc tctgccaaaa attatgggga
      661 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
      721 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
      781 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
      841 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
      901 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
      961 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     1021 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     1081 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctgggtgc
     1201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1261 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     1321 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1381 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     1441 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     1501 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1561 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     1621 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1741 tcactcaaag gcggtaatac ggttatccac agaatcaggg ataacgcagg aaagaacatg
     1801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2281 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     2641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     2701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     3241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3601 ccacctgggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     3661 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3721 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3781 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     3841 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     3901 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     3961 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     4021 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     4081 ttgtgcagcg atgggggcgg gggggggggg ggggcccccc ccaggcgggg cggggcgggg
     4141 cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca gccaatcaga gcggcgcgct
     4201 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     4261 gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg ccccgctccg ccgccgcctc
     4321 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     4381 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     4441 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     4501 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     4561 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     4621 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     4681 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     4741 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     4801 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     4861 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaaggggcgc ggcggccccc
     4921 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     4981 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     5041 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     5101 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     5161 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     5221 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     5281 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaa
//
