LOCUS       V008871                 6071 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008871
VERSION     V008871
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6071)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6071)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..51
                     /label="E-tag"
                     /note="E-tag"
     CDS             67..1356
                     /gene="Tnip2"
                     /label="TNFAIP3-interacting protein 2"
                     /note="TNFAIP3-interacting protein 2 from Mus musculus.
                     Accession#: Q99JG7"
     polyA_signal    1428..1483
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1844..1860)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1868..1884)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1892..1922)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1936..1957)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2015..2211
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2217..2351
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2589..3177)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3351..4208)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4209..4313)
                     /label="AmpR promoter"
     enhancer        4344..4723
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4344..4721
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4725..5002
                     /label="chicken beta-actin promoter"
     intron          5003..6020
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggg
       61 atccgtatgt cgtctgggga cccaaggtct ggtagacagg acggggcccc gcgtgcggcc
      121 gcagcgctct gtggcctgta ccacgaggcc ggccagcaac tacagcgcct gaaggatcag
      181 ctggccgcgc gtgacgccct catcgcgagc ctccgcaccc gcctcgcggc tctggaaggg
      241 cacacggcgc cgtcactcgt ggacgcactt ctggatcagg tggagcgctt ccgtgagcag
      301 ctgcgacgac aggaggaagg cgcttcggag acccagctgc ggcaggaagt tgaaagactt
      361 acggagcgtc tagaggaaaa agagagggag atgcaacagc tgatgagcca gcctcagcat
      421 gagcaagaga aggaggtagt cttgcttcgg cgaagtgtgg cagagaagga gaaagccagg
      481 gccgccagtg atgttctgtg ccgctccttg gctgatgaga cccaccaact gcgcaggaca
      541 ttggcagcca ctgcccacat gtgccaacat ctggccaaat gtctggatga acgacagtgt
      601 gcacagggag acgctgggga gaaaagccct gctgagctag agcaaacaag cagcgatgct
      661 tctggccaga gtgttattaa gaagttacag gaagaaaatc gactgttaaa acagaaggtg
      721 actcatgtag aagacctcaa tgctaagtgg cagcgttatg atgcaagtag ggacgaatat
      781 gtgaaggggt tgcatgccca gctaaagagg cggcaggtcc ctctggagcc tgagctgatg
      841 aagaaggaga tttcccgact taacagacag ttggaggaga aaataagtga ctgtgcggaa
      901 gcaaaccagg agctgacagc catgaggatg tcccgggaca ctgcgctgga gcgagtgcag
      961 atgctagaac agcagattct tgcttacaag gatgacttca aatcagaaag ggcagatcgg
     1021 gaacgagcgc acagtaggat tcaagagctg gaggaaaaga tcatgtcctt gatgtaccaa
     1081 gtgtcccaga gacaggactc ccgggagcca ggaccctgtc ggattcatac ggggaacaaa
     1141 actgccaagt acttagagat ggatgcactg gagcatgtga cccctggcgg ctggaggcct
     1201 gagtctaggt cccaacagat ggaaccttct gcagagggtg ggcatgtgtg cacagcccag
     1261 agaggtcagg gtgaccttca gtgccctcat tgcctgcggt gcttcagtga tgagcaaggc
     1321 gaggcattcc tcaggcacct gtctgagtgc tgccaatgaa gatctttttc cctctgccaa
     1381 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     1441 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     1501 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     1561 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     1621 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     1681 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     1741 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     1801 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     1861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1921 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1981 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     2041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     2161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     2221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     2281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     2341 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca
     2521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4321 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
     4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     4681 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
     4741 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     4801 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg
     4861 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca
     4921 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     4981 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc
     5041 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     5101 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
     5161 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
     5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
     5281 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
     5341 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
     5401 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
     5461 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
     5521 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
     5581 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc
     5641 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
     5701 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
     5761 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
     5821 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     5881 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
     5941 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
     6001 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     6061 ttggcaaaga a
//
