LOCUS       V008872                 7258 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008872
VERSION     V008872
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7258)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7258)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..49
                     /label="E-tag"
                     /note="E-tag"
     CDS             59..2452
                     /gene="CSDE1"
                     /label="Cold shock domain-containing protein E1"
                     /note="Cold shock domain-containing protein E1 from Homo
                     sapiens. Accession#: O75534"
     polyA_signal    2610..2665
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3026..3042)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3050..3066)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3074..3104)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3118..3139)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3197..3393
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    3399..3533
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3771..4359)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4533..5390)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5391..5495)
                     /label="AmpR promoter"
     enhancer        5526..5905
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5526..5903
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5907..6184
                     /label="chicken beta-actin promoter"
     intron          6185..7202
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat
       61 gagctttgat ccaaaccttc tccacaacaa tggacataat gggtacccta atggtacttc
      121 agcagcactg cgtgaaactg gggttattga aaaactgtta acctcttacg gatttattca
      181 gtgttcagaa cgtcaagcta gacttttctt ccactgttca cagtataatg gcaacctgca
      241 agacttaaaa gtaggagatg atgttgaatt tgaagtatca tcggaccgac ggactgggaa
      301 acccattgct gttaaactgg tgaagataaa acaagaaatc ctccctgaag aacgaatgaa
      361 tggacaagtt gtgtgcgctg ttcctcacaa cttagagagt aaatctccag ctgccccggg
      421 tcagagtcca acagggagtg tatgctacga acgtaatggg gaagtgtttt atctgactta
      481 cacccctgaa gatgtcgaag ggaacgttca gctggaaact ggagataaaa taaactttgt
      541 aattgataac aataaacata ctggtgctgt aagtgctcgc aacattatgc tgttgaaaaa
      601 gaaacaagcc cgctgtcagg gagtagtttg tgccatgaag gaggcatttg gctttattga
      661 aagaggtgat gttgtaaaag agatattctt tcactatagt gaatttaagg gtgacttaga
      721 aaccttacag cctggcgatg atgtggaatt cacaatcaag gacagaaatg gtaaagaagt
      781 tgcaacagat gtcagactat tgcctcaagg aacagtcatt tttgaagata tcagcattga
      841 acattttgaa ggaactgtaa ccaaagttat cccaaaagta cccagtaaaa accagaatga
      901 cccattgcca ggacgcatca aagttgactt tgtgatccct aaagaacttc cctttggaga
      961 caaagatacg aaatccaagg tgaccctgct ggaaggtgac catgttaggt ttaatatttc
     1021 aacagaccga cgtgacaaat tagagcgagc aaccaatata gaagttctgt caaatacatt
     1081 tcagttcact aatgaagccc gagaaatggg tgtgattgct gccatgagag atggttttgg
     1141 tttcatcaag tgtgtggatc gtgatgttcg tatgttcttc cacttcagtg aaattctgga
     1201 tgggaaccag ctccatattg cagatgaagt agagtttact gtggttcctg atatgctctc
     1261 tgctcaaaga aatcatgcta ttaggattaa aaaacttccc aagggcacgg tttcatttca
     1321 ttcccattca gatcaccgtt ttctgggcac ggtagaaaaa gaagccactt tttccaatcc
     1381 taaaaccact agcccaaata aaggcaaaga gaaggaggct gaggatggca ttattgctta
     1441 tgatgactgt ggggtgaaac tgactattgc ttttcaagcc aaggatgtgg aaggatctac
     1501 ttctcctcaa ataggagata aggttgaatt tagtattagt gacaaacaga ggcctggaca
     1561 gcaggttgca acttgtgtgc gacttttagg tcgtaattct aactccaaga ggctcttggg
     1621 ttatgtggca actctgaagg ataattttgg atttattgaa acagccaatc atgataagga
     1681 aatctttttc cattacagtg agttctctgg tgatgttgat agcctggaac tgggggacat
     1741 ggtcgagtat agcttgtcca aaggcaaagg caacaaagtc agtgcagaaa aagtgaacaa
     1801 aacacactca gtgaatggca ttactgagga agctgatccc accatttact ctggcaaagt
     1861 aattcgcccc ctgaggagtg ttgatccaac acagactgag taccaaggaa tgattgagat
     1921 tgtggaggag ggcgatatga aaggtgaggt ctatccattt ggcatcgttg ggatggccaa
     1981 caaaggggat tgcctgcaga aaggggagag cgtcaagttc caattgtgtg tcctgggcca
     2041 aaatgcacaa actatggctt acaacatcac acccctgcgc agggccacag tggaatgtgt
     2101 gaaagatcag tttggcttca ttaactatga agtaggagat agcaagaagc tctttttcca
     2161 tgtgaaagaa gttcaggatg gcattgagct acaggcagga gatgaggtgg agttctcagt
     2221 gattcttaat cagcgcactg gcaagtgcag cgcctgtaat gtttggcgag tctgtgaggg
     2281 ccccaaggct gttgcagctc ctcgacctga tcggttggtc aatcgcttga agaatatcac
     2341 tctggatgat gccagtgctc ctcgcctaat ggttcttcgt cagccaaggg gaccagataa
     2401 ctcaatgggg tttggtgcag aaagaaagat ccgtcaagct ggtgtcattg attaactcga
     2461 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2521 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2581 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2641 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2701 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     2761 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     2821 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     2881 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     2941 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     3001 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3061 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg
     3121 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3181 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3361 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     3421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     3541 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3661 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca
     3721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5521 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     5581 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     5641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     5701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     5761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     5821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     5881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     5941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     6001 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
     6061 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
     6121 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
     6181 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
     6241 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     6301 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     6361 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     6421 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     6481 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     6541 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     6601 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     6661 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     6721 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     6781 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
     6841 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     6901 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     6961 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     7021 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     7081 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     7141 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     7201 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgaa
//
