LOCUS       V008873                 7025 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008873
VERSION     V008873
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7025)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7025)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..2196
                     /gene="TICAM1"
                     /label="TIR domain-containing adapter molecule 1"
                     /note="TIR domain-containing adapter molecule 1 from Homo
                     sapiens. Accession#: Q8IUC6"
     polyA_signal    2382..2437
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2798..2814)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2822..2838)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2846..2876)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2890..2911)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2969..3165
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    3171..3305
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3543..4131)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4305..5162)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5163..5267)
                     /label="AmpR promoter"
     enhancer        5298..5677
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5298..5675
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5679..5956
                     /label="chicken beta-actin promoter"
     intron          5957..6974
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgcctgca caggcccatc acttcctagc gccttcgaca ttctaggtgc agcaggccag
      121 gacaagctct tgtatctgaa gcacaaactg aagaccccac gcccaggctg ccaggggcag
      181 gacctcctgc atgccatggt tctcctgaag ctgggccagg aaactgaggc caggatctct
      241 ctagaggcat tgaaggccga tgcggtggcc cggctggtgg cccgccagtg ggctggcgtg
      301 gacagcaccg aggacccaga ggagccccca gatgtgtcct gggctgtggc ccgcttgtac
      361 cacctgctgg ctgaggagaa gctgtgcccc gcctcgctgc gggacgtggc ctaccaggaa
      421 gccgtccgca ccctcagctc cagggacgac caccggctgg gggaacttca ggatgaggcc
      481 cgaaaccggt gtgggtggga cattgctggg gatccaggga gcatccggac gctccagtcc
      541 aatctgggct gcctcccacc atcctcggct ttgccctctg ggaccaggag cctcccacgc
      601 cccattgacg gtgtttcgga ctggagccaa gggtgctccc tgcgatccac tggcagccct
      661 gcctccctgg ccagcaactt ggaaatcagc cagtccccta ccatgccctt cctcagcctg
      721 caccgcagcc cacatgggcc cagcaagctc tgtgacgacc cccaggccag cttggtgccc
      781 gagcctgtcc ccggtggctg ccaggagcct gaggagatga gctggccgcc atcgggggag
      841 attgccagcc caccagagct gccaagcagc ccacctcctg ggcttcccga agtggcccca
      901 gatgcaacct ccactggcct ccctgatacc cccgcagctc cagaaaccag caccaactac
      961 ccagtggagt gcaccgaggg gtctgcaggc ccccagtctc tccccttgcc tattctggag
     1021 ccggtcaaaa acccctgctc tgtcaaagac cagacgccac tccaactttc tgtagaagat
     1081 accacctctc caaataccaa gccgtgccca cctactccca ccaccccaga aacatcccct
     1141 cctcctcctc ctcctcctcc ttcatctact ccttgttcag ctcacctgac cccctcctcc
     1201 ctgttccctt cctccctgga atcatcatcg gaacagaaat tctataactt tgtgatcctc
     1261 cacgccaggg cagacgaaca catcgccctg cgggttcggg agaagctgga ggcccttggc
     1321 gtgcccgacg gggccacctt ctgcgaggat ttccaggtgc cggggcgcgg ggagctgagc
     1381 tgcctgcagg acgccataga ccactcagct ttcatcatcc tacttctcac ctccaacttc
     1441 gactgtcgcc tgagcctgca ccaggtgaac caagccatga tgagcaacct cacgcgacag
     1501 gggtcgccag actgtgtcat ccccttcctg cccctggaga gctccccggc ccagctcagc
     1561 tccgacacgg ccagcctgct ctccgggctg gtgcggctgg acgaacactc ccagatcttc
     1621 gccaggaagg tggccaacac cttcaagccc cacaggcttc aggcccgaaa ggccatgtgg
     1681 aggaaggaac aggacacccg agccctgcgg gaacagagcc aacacctgga cggtgagcgg
     1741 atgcaggcgg cggcactgaa cgcagcctac tcagcctacc tccagagcta cttgtcctac
     1801 caggcacaga tggagcagct ccaggtggct tttgggagcc acatgtcatt tgggactggg
     1861 gcgccctatg gggctcgaat gccctttggg ggccaggtgc ccctgggagc cccgccaccc
     1921 tttcccactt ggccggggtg cccgcagccg ccacccctgc acgcatggca ggctggcacc
     1981 cccccaccgc cctccccaca gccagcagcc tttccacagt cactgccctt cccgcagtcc
     2041 ccagccttcc ctacggcctc acccgcaccc cctcagagcc cagggctgca acccctcatt
     2101 atccaccacg cacagatggt acagctgggg ctgaacaacc acatgtggaa ccagagaggg
     2161 tcccaggcgc ccgaggacaa gacgcaggag gcagaatgac ccgggacatt aaacgcgtgg
     2221 gtaccggatc cggcaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     2281 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     2341 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     2401 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2461 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     2521 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     2581 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     2641 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     2701 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     2761 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2941 gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg
     3001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta
     3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3301 ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3361 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3421 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag
     3481 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3541 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3601 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3661 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3721 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3781 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3841 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3901 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3961 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4021 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4081 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4141 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4201 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4261 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4321 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4381 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4441 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4501 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4561 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4621 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4681 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4741 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4801 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4861 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4921 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4981 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5041 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5101 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5161 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5221 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5281 aaagtgccac ctgggtcgac attgattatt gactagttat taatagtaat caattacggg
     5341 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     5401 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     5461 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     5521 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     5581 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     5641 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     5701 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     5761 attattttgt gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg
     5821 gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg
     5881 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
     5941 aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc
     6001 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     6061 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     6121 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     6181 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     6241 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     6301 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     6361 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     6421 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     6481 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     6541 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg
     6601 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     6661 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     6721 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     6781 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     6841 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     6901 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     6961 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     7021 aagaa
//
