LOCUS       V008874                 5527 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008874
VERSION     V008874
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5527)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..768
                     /gene="TICAM2"
                     /label="TIR domain-containing adapter molecule 2"
                     /note="TIR domain-containing adapter molecule 2 from Homo
                     sapiens. Accession#: Q86XR7"
     polyA_signal    880..935
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1296..1312)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1320..1336)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1344..1374)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1388..1409)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1467..1663
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1669..1803
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2041..2629)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2803..3660)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3661..3765)
                     /label="AmpR promoter"
     enhancer        3796..4175
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3796..4173
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4177..4454
                     /label="chicken beta-actin promoter"
     intron          4455..5476
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gcaatgggta tcgggaagtc taaaataaat tcctgccctc tttctctctc ttggggtaaa
      121 aggcacagtg tggatacaag tccaggatat catgagtcag attccaagaa gtctgaagat
      181 ctatccttgt gtaatgttgc tgagcacagc aatacaacag aggggccaac aggaaagcag
      241 gagggagctc agagcgtgga agagatgttt gaagaagaag ctgaagaaga ggtgttcctc
      301 aaatttgtga tattgcatgc agaagatgac acagatgaag ccctcagagt ccagaatctg
      361 ctacaagatg actttggtat caaacccgga ataatctttg ctgagatgcc atgtggcaga
      421 cagcatttac agaatttaga tgatgctgta aatgggtctg catggacaat cttattactg
      481 actgaaaact ttttaagaga tacttggtgt aatttccagt tctatacgtc cctaatgaac
      541 tccgttaaca ggcagcataa atacaactct gttataccca tgcggcccct gaacaatccc
      601 cttccccgag aaaggactcc ctttgccctc caaaccatca atgccttaga ggaagaaagt
      661 cgtggatttc ctacacaagt agaaagaatt tttcaggagt ctgtgtataa gacacaacaa
      721 actatatgga aagagacaag aaatatggta caaagacaat ttattgcctg actcgagctg
      781 cagccaatgc cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg
      841 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
      901 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
      961 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
     1021 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
     1081 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     1141 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     1201 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
     1261 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1321 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1441 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     1501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     1561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     1621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     1681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1801 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1861 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1921 cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa
     1981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2221 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2461 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3781 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
     3841 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3901 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3961 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4021 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4081 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4141 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
     4201 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
     4261 tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc
     4321 ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg
     4381 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     4441 gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg
     4501 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     4561 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     4621 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     4681 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     4741 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     4801 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     4861 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     4921 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     4981 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     5041 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc
     5101 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     5161 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     5221 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     5281 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     5341 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     5401 ggcttctggc gtgtgaccgg cggctctagc tagagcctct gctaaccatg ttcatgcctt
     5461 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
     5521 caaagaa
//
