LOCUS       40924_8141        6373 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-E-hPTBdelnls, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6373)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..49
                     /label=E-tag
                     /note="E-tag"
     CDS             59..1570
                     /codon_start=1
                     /note="unnamed protein product; hPTBdelnls"
                     /protein_id="SJL87136.1"
                     /translation="MVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFI
                     EMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQ
                     SGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFT
                     KNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYT
                     RPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALA
                     PLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVK
                     ILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQGLT
                     KDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVKGFKF
                     FQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI"
     polyA_signal    1725..1780
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2141..2157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2165..2181)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2189..2219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2233..2254)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2312..2508
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    2514..2648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2886..3474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3648..4505)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4506..4610)
                     /label=AmpR promoter
     enhancer        4641..5020
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4641..5018
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5022..5299
                     /label=chicken beta-actin promoter
     intron          5300..6317
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat
       61 ggtcccctct agagtgatcc acatccggaa gctccccatc gacgtcacgg agggggaagt
      121 catctccctg gggctgccct ttgggaaggt caccaacctc ctgatgctga aggggaaaaa
      181 ccaggccttc atcgagatga acacggagga ggctgccaac accatggtga actactacac
      241 ctcggtgacc cctgtgctgc gcggccagcc catctacatc cagttctcca accacaagga
      301 gctgaagacc gacagctctc ccaaccaggc gcgggcccag gcggccctgc aggcggtgaa
      361 ctcggtccag tcggggaacc tggccttggc tgcctcggcg gcggccgtgg acgcagggat
      421 ggcgatggcc gggcagagcc ccgtgctcag gatcatcgtg gagaacctct tctaccctgt
      481 gaccctggat gtgctgcacc agattttctc caagttcggc acagtgttga agatcatcac
      541 cttcaccaag aacaaccagt tccaggccct gctgcagtat gcggaccccg tgagcgccca
      601 gcacgccaag ctgtcgctgg acgggcagaa catctacaac gcctgctgca cgctgcgcat
      661 cgacttttcc aagctcacca gcctcaacgt caagtacaac aatgacaaga gccgtgacta
      721 cacacgccca gacctgcctt ccggggacag ccagccctcg ctggaccaga ccatggccgc
      781 ggccttcggt gcacctggta taatctcagc ctctccgtat gcaggagctg gtttccctcc
      841 cacctttgcc attcctcaag ctgcaggcct ttccgttccg aacgtccacg gcgccctggc
      901 ccccctggcc atcccctcgg cggcggcggc agctgcggcg gcaggtcgga tcgccatccc
      961 gggcctggcg ggggcaggaa attctgtatt gctggtcagc aacctcaacc cagagagagt
     1021 cacaccccaa agcctcttta ttcttttcgg cgtctacggt gacgtgcagc gcgtgaagat
     1081 cctgttcaat aagaaggaga acgccctagt gcagatggcg gacggcaacc aggcccagct
     1141 ggccatgagc cacctgaacg ggcacaagct gcacgggaag cccatccgca tcacgctctc
     1201 gaagcaccag aacgtgcagc tgccccgcga gggccaggag gaccagggcc tgaccaagga
     1261 ctacggcaac tcacccctgc accgcttcaa gaagccgggc tccaagaact tccagaacat
     1321 attcccgccc tcggccacgc tgcacctctc caacatcccg ccctcagtct ccgaggagga
     1381 tctcaaggtc ctgttttcca gcaatggggg cgtcgtcaaa ggattcaagt tcttccagaa
     1441 ggaccgcaag atggcactga tccagatggg ctccgtggag gaggcggtcc aggccctcat
     1501 tgacctgcac aaccacgacc tcggggagaa ccaccacctg cgggtctcct tctccaagtc
     1561 caccatctag ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     1621 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     1681 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     1741 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     1801 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     1861 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     1921 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     1981 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     2041 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     2101 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2161 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2221 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     2281 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc
     2341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     2401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     2461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt
     2521 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2581 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2641 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga
     2821 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3301 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3361 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3961 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4261 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4621 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac
     4681 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4741 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4801 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4861 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4921 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4981 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     5041 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     5101 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     5161 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     5221 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     5281 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     5341 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     5401 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     5461 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     5521 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     5581 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     5641 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     5701 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     5761 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     5821 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     5881 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     5941 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     6001 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     6061 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     6121 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     6181 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     6241 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     6301 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     6361 gcaaagaatt gaa
//
