LOCUS       V008876                 6535 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008876
VERSION     V008876
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6535)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..49
                     /label="E-tag"
                     /note="E-tag"
     CDS             59..1729
                     /gene="PTBP1"
                     /label="Polypyrimidine tract-binding protein 1"
                     /note="Polypyrimidine tract-binding protein 1 from Homo
                     sapiens. Accession#: P26599"
     polyA_signal    1887..1942
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2303..2319)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2327..2343)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2351..2381)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2395..2416)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2474..2670
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2676..2810
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3048..3636)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3810..4667)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4668..4772)
                     /label="AmpR promoter"
     enhancer        4803..5182
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4803..5180
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5184..5461
                     /label="chicken beta-actin promoter"
     intron          5462..6479
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgctat
       61 ggacggcatt gtcccagata tagccgttgg tacaaagcgg ggatctgacg agcttttctc
      121 tacttgtgtc actaacggac cgtttatcat gagcagcaac tcggcttctg cagcaaacgg
      181 aaatgacagc aagaagttca aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat
      241 ccacatccgg aagctcccca tcgacgtcac ggagggggaa gtcatctccc tggggctgcc
      301 ctttgggaag gtcaccaacc tcctgatgct gaaggggaaa aaccaggcct tcatcgagat
      361 gaacacggag gaggctgcca acaccatggt gaactactac acctcggtga cccctgtgct
      421 gcgcggccag cccatctaca tccagttctc caaccacaag gagctgaaga ccgacagctc
      481 tcccaaccag gcgcgggccc aggcggccct gcaggcggtg aactcggtcc agtcggggaa
      541 cctggccttg gctgcctcgg cggcggccgt ggacgcaggg atggcgatgg ccgggcagag
      601 ccccgtgctc aggatcatcg tggagaacct cttctaccct gtgaccctgg atgtgctgca
      661 ccagattttc tccaagttcg gcacagtgtt gaagatcatc accttcacca agaacaacca
      721 gttccaggcc ctgctgcagt atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct
      781 ggacgggcag aacatctaca acgcctgctg cacgctgcgc atcgactttt ccaagctcac
      841 cagcctcaac gtcaagtaca acaatgacaa gagccgtgac tacacacgcc cagacctgcc
      901 ttccggggac agccagccct cgctggacca gaccatggcc gcggccttcg gtgcacctgg
      961 tataatctca gcctctccgt atgcaggagc tggtttccct cccacctttg ccattcctca
     1021 agctgcaggc ctttccgttc cgaacgtcca cggcgccctg gcccccctgg ccatcccctc
     1081 ggcggcggcg gcagctgcgg cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg
     1141 aaattctgta ttgctggtca gcaacctcaa cccagagaga gtcacacccc aaagcctctt
     1201 tattcttttc ggcgtctacg gtgacgtgca gcgcgtgaag atcctgttca ataagaagga
     1261 gaacgcccta gtgcagatgg cggacggcaa ccaggcccag ctggccatga gccacctgaa
     1321 cgggcacaag ctgcacggga agcccatccg catcacgctc tcgaagcacc agaacgtgca
     1381 gctgccccgc gagggccagg aggaccaggg cctgaccaag gactacggca actcacccct
     1441 gcaccgcttc aagaagccgg gctccaagaa cttccagaac atattcccgc cctcggccac
     1501 gctgcacctc tccaacatcc cgccctcagt ctccgaggag gatctcaagg tcctgttttc
     1561 cagcaatggg ggcgtcgtca aaggattcaa gttcttccag aaggaccgca agatggcact
     1621 gatccagatg ggctccgtgg aggaggcggt ccaggccctc attgacctgc acaaccacga
     1681 cctcggggag aaccaccacc tgcgggtctc cttctccaag tccaccatct agctcgagga
     1741 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
     1801 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     1861 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     1921 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     1981 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     2041 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     2101 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     2161 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     2221 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     2281 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2341 caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg cctaatgagt
     2401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2461 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2521 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2581 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     2641 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     2701 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2761 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     2821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2941 ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat gtgagcaaaa
     3001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg
     4801 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     4861 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4921 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4981 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     5041 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     5101 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     5161 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     5221 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     5281 gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg
     5341 gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     5401 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     5461 gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc
     5521 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     5581 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     5641 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     5701 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     5761 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     5821 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     5881 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     5941 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     6001 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     6061 ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg
     6121 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     6181 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     6241 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     6301 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     6361 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     6421 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     6481 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgaa
//
