LOCUS       40924_8126        5752 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-E-hABIN-3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5752)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5752)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label=E-tag
                     /note="E-tag"
     CDS             64..1023
                     /codon_start=1
                     /note="unnamed protein product; hABIN-3"
                     /protein_id="SJL87743.1"
                     /translation="MAHFVQGTSRMIAAESSTEHKECAEPSTRKNLMNSLEQKIRCLEK
                     QRKELLEVNQQWDQQFRSMKELYERKVAELKTKLDAAERFLSTREKDPHQRQRKDDRQR
                     EDDRQRDLTRDRLQREEKEKERLNEELHELKEENKLLKGKNTLANKEKEHYECEIKRLN
                     KALQDALNIKCSFSEDCLRKSRVEFCHEEMRTEMEVLKQQVQIYEEDFKKERSDRERLN
                     QEKEELQQINETSQSQLNRLNSQIKACQMEKEKLEKQLKQMYCPPCNCGWFSTCKIHGY
                     QQALELCRSNGSTHQTISGMLLTSFRQMYNTRQMVYPQ"
     polyA_signal    1109..1164
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1525..1541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1549..1565)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1573..1603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1617..1638)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1696..1892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1898..2032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2270..2858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3032..3889)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3890..3994)
                     /label=AmpR promoter
     enhancer        4025..4404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4025..4402
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4406..4683
                     /label=chicken beta-actin promoter
     intron          4684..5701
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctatggcac attttgtaca aggcacatct agaatgattg ccgcagaaag ttctacggag
      121 cataaagagt gtgctgaacc atcaacaaga aagaacttga tgaattctct tgaacaaaag
      181 ataaggtgtt tggaaaaaca aagaaaagag ctcctggaag ttaaccagca atgggatcag
      241 caatttagaa gtatgaaaga gttatatgaa agaaaggtag cagagctgaa gacgaaactg
      301 gacgccgcgg aaagattcct cagcacgcgg gagaaggatc cgcatcagag gcagagaaag
      361 gacgacaggc agagagagga cgacaggcag cgcgacctga cccgggaccg gctgcagcgg
      421 gaggagaagg aaaaggaacg cctaaatgaa gaattacatg aattgaaaga agagaataaa
      481 cttttaaagg gaaaaaatac tcttgcgaac aaggaaaagg aacattacga atgtgaaata
      541 aaacgcctca ataaggctct tcaggatgcc ttgaatatca agtgttcatt ttccgaggac
      601 tgtttgagga agtctcgagt ggaattctgc catgaggaga tgagaacaga aatggaagtt
      661 ctgaagcagc aggtgcaaat atacgaagaa gacttcaaaa aggaacgatc ggatcgagag
      721 agacttaatc aagagaaaga ggagctacag caaattaatg aaacttccca atcccagttg
      781 aacaggctga attcccagat aaaagcttgt cagatggaga aagaaaaact agaaaagcaa
      841 ttaaaacaga tgtattgccc accctgtaac tgcggctggt tttccacctg caagatccat
      901 gggtaccaac aggccctgga gctgtgcaga agcaacggga gcacccacca gactatcagt
      961 ggtatgctct tgaccagctt ccgccagatg tacaacacaa ggcaaatggt ttatcctcag
     1021 taaagaaagt ccatccgtag agatcttttt ccctctgcca aaaattatgg ggacatcatg
     1081 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     1141 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     1201 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     1261 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     1321 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     1381 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     1441 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     1501 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg tgcctaatga
     1621 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1681 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     1741 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     1801 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     1861 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     1921 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1981 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     2041 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2101 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2161 aaggcggtaa tacggttatc cacagaatca gggataacgc aggaaagaac atgtgagcaa
     2221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     3061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     3121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     3181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     3241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     4021 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     4081 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     4141 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     4201 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     4261 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     4321 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     4381 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     4441 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     4501 gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg gggcgagggg
     4561 cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     4621 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     4681 gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc
     4741 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     4801 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     4861 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     4921 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     4981 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     5041 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     5101 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     5161 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     5221 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     5281 gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc cccggagcgc
     5341 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     5401 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     5461 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     5521 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     5581 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     5641 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     5701 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aa
//
