LOCUS       V008879                 6557 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008879
VERSION     V008879
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6557)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..51
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1350
                     /gene="TNIP2"
                     /label="TNFAIP3-interacting protein 2"
                     /note="TNFAIP3-interacting protein 2 from Homo sapiens.
                     Accession#: Q8NFZ5"
     polyA_signal    1914..1969
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2330..2346)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2354..2370)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2378..2408)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2422..2443)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2501..2697
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2703..2837
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3075..3663)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3837..4694)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4695..4799)
                     /label="AmpR promoter"
     enhancer        4830..5209
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4830..5207
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5211..5488
                     /label="chicken beta-actin promoter"
     intron          5489..6506
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggt
       61 accatgtccc gggacccggg gtcgggcggc tgggaggagg ccccgcgcgc agctgccgcg
      121 ctctgcaccc tgtaccacga ggccggacag cggctgcgcc gcctgcagga ccagctcgct
      181 gcccgcgacg ccctcatcgc tcgcctccgc gcccgcctgg ccgcgctgga gggggacgcc
      241 gcgccgtccc tagtggacgc gctgctggag caggttgcgc gcttccggga gcagctgcga
      301 aggcaggagg gcggcgccgc cgaggcccag atgcgccagg aaattgagag gctgactgag
      361 cgactagaag aaaaagagag ggagatgcag cagctgctga gccagcccca acacgagcga
      421 gagaaggaag tcgtcctgct acggaggagc atggcagaag gggagcgcgc ccgggccgcc
      481 agtgacgtcc tgtgccgctc cttggccaac gagacccatc atctgcggag gacgctgacc
      541 gccaccgccc acatgtgtca gcatctggcc aagtgtctgg atgaacgaca gcatgcacaa
      601 aggaatgtgg gggagagaag tcctgaccag tcggaacaca cagatgggca cacctctgtc
      661 cagagtgtta ttgagaagtt gcaggaagaa aatcgactgt taaaacagaa ggtgactcac
      721 gttgaagacc tcaatgccaa gtggcagcgc tacaacgcca gcagggacga atacgtgagg
      781 gggctccatg cgcagctcag ggggctgcag atcccccacg agcccgagct gatgaggaag
      841 gagatctccc ggctcaacag acagttggaa gagaaaataa atgactgtgc cgaagtgaag
      901 caggagctgg cggcctccag gacggcccgg gatgctgcgt tggagcgggt gcagatgctg
      961 gaacagcaga ttctcgctta caaggatgac ttcatgtcag aaagggccga tcgggaacgg
     1021 gctcaaagta ggattcaaga actggaggaa aaggtcgcct ctttgctgca ccaggtgtcc
     1081 tggagacagg attctcgaga gccagacgcc ggccggattc acgctgggag caaaactgcc
     1141 aagtatttgg ccgccgacgc attagagctt atggtgcctg gtggctggag gcctgggact
     1201 gggtcccagc agccagaacc ccctgcagag ggcgggcatc ctggcgcggc ccagagaggc
     1261 cagggggacc ttcagtgccc tcactgcctg cagtgcttca gtgacgagca aggggaagag
     1321 ctcctcaggc atgtggccga gtgctgccag tgaccgagac tcacccgtgc ccttgcggcc
     1381 tcctggcccg gtgcagctgc cctcagggac agggtgggtg ctctcagatg ccatgggttg
     1441 agctctactg agagccaagg cccctagaat agttgcgggg cactctgatc gttcactttg
     1501 gtccctttgg ctatggaaca ggctgggtca cagggaactg ccagtgaggc tggaggctgg
     1561 aggtggagat ggggtcagga acatctggca gagggaggtc ccagtctgtg tctccatcag
     1621 gcttaagcca gagctatctg gtgctggtgt gccagcccct cccccagcct gcctagaaag
     1681 gggtggctgc ctgagggagt cacttgtatg gtccccaggg tgggagcccc atcctgttct
     1741 atggaataaa gcgtcgcctc tctgcctcga accagtcaaa tggagtattg cggctgcacg
     1801 tcacactagg gtggccaccc ccgccattgc gagccacatg tctgcggatc tttttccctc
     1861 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     1921 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     1981 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     2041 atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat
     2101 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     2161 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     2221 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     2281 tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     2341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2401 agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     2461 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     2521 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     2581 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     2641 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     2701 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2761 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2821 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggat
     3001 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3061 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3121 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3181 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3241 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3301 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3361 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     3421 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     3481 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3541 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3601 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3661 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3721 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3781 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3841 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3901 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3961 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4021 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4081 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4141 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4201 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4261 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4321 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4381 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     4441 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4501 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4561 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4621 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4681 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4741 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4801 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta
     4861 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4921 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4981 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     5041 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     5101 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     5161 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
     5221 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
     5281 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcccccccc
     5341 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggaaaggtg cggcggcagc
     5401 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     5461 ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc
     5521 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     5581 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     5641 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     5701 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     5761 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     5821 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     5881 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     5941 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     6001 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     6061 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     6121 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     6181 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     6241 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     6301 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     6361 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     6421 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     6481 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     6541 atcattttgg caaagaa
//
