LOCUS       V008880                 6772 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008880
VERSION     V008880
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6772)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..49
                     /label="E-tag"
                     /note="E-tag"
     CDS             59..1966
                     /gene="TNIP1"
                     /label="TNFAIP3-interacting protein 1"
                     /note="TNFAIP3-interacting protein 1 from Homo sapiens.
                     Accession#: Q15025"
     polyA_signal    2124..2179
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2540..2556)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2564..2580)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2588..2618)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2632..2653)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2711..2907
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2913..3047
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3285..3873)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4047..4904)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4905..5009)
                     /label="AmpR promoter"
     enhancer        5040..5419
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5040..5417
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5421..5698
                     /label="chicken beta-actin promoter"
     intron          5699..6716
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgccat
       61 ggaagggaga ggaccgtacc ggatttacga ccctgggggc agcgtgccct caggagaggc
      121 atccgcagct tttgagcgcc tagtgaagga gaattcccgg ctgaaggaaa aaatgcaagg
      181 gataaagatg ttaggggagc ttttggaaga gtcccagatg gaagcgacca ggctccggca
      241 gaaggcagag gagctagtga aggacaacga gctgctccca ccaccttctc cctccttggg
      301 ctccttcgac cccctggctg agctcacagg aaaggactca aatgtcacag catctcccac
      361 agcccctgca tgccccagtg acaagccagc accagtccag aagcctccat ccagtggcac
      421 ctcctctgaa tttgaagtgg tcactcctga ggagcagaat tcaccagaga gcagcagcca
      481 tgccaatgcg atggcgctgg gccccctgcc ccgtgaggac ggcaacctga tgctgcacct
      541 gcagcgcctg gagaccacgc tgagtgtgtg tgccgaggag ccggaccacg gccagctctt
      601 cacccacctg ggccgcatgg ccctggagtt caaccgactg gcatccaagg tgcacaagaa
      661 tgagcagcgc acctccattc tgcagaccct gtgtgagcag cttcggaagg agaacgaggc
      721 tctgaaggcc aagttggata agggcctgga acagcgggat caggctgccg agaggctgcg
      781 ggaggaaaat ttggagctca agaagttgtt gatgagcaat ggcaacaaag agggtgcgtc
      841 tgggcggcca ggctcaccga agatggaagg gacaggcaag aaggcagtgg ctggacagca
      901 gcaggctagt gtgacggcag gtaaggtccc agaggtggtg gccttgggcg cacccgagaa
      961 gaaggtgaag atgctggagc agcagcgcag tgagctgctg gaagtgaaca agcagtggga
     1021 ccagcatttc cggtccatga agcagcagta tgagcagaag atcactgagc tgcgtcagaa
     1081 gctggctgat ttgcagaagc aggtgactga cctggaggcc gagcgggagc agaagcagcg
     1141 tgactttgac cgcaagctcc tcctggccaa gtccaagatt gaaatggagg agaccgacaa
     1201 ggagcagctg acagcagagg ccaaggagct gcgccaaaag gtcaagtacc tgcaggatca
     1261 gctgagccca ctcacccgac agcgtgagta ccaggaaaag gagatccagc ggctcaacaa
     1321 ggccctggag gaagcactga gcatccaaac cccgccatca tctccaccaa cagcatttgg
     1381 gagcccagaa ggagcagggg ccctcctaag gaaacaggag ctggtcacgc agaatgagtt
     1441 gctgaaacag caggtgaaga tcttcgagga ggacttccag agggagcgca gtgatcgtga
     1501 gcgcatgaat gaggagaagg aagagctgaa gaagcaagtg gagaagctgc aggcccaggt
     1561 caccctgtca aatgcccagc taaaagcatt caaagatgag gagaaggcaa gagaagccct
     1621 cagacagcag aagaggaaag caaaggcctc aggagagcgt taccatgtgg agccccaccc
     1681 agaacatctc tgcggggcct acccctacgc ctacccgccc atgccagcca tggtgccaca
     1741 ccatggcttc gaggactggt cccagatccg ctacccccct ccccccatgg ccatggagca
     1801 cccgccccca ctccccaact cgcgcctctt ccatctgccg gaatacacct ggcgtctacc
     1861 ctgtggaggg gttcgaaatc caaatcagag ctcccaagtg atggaccctc ccacagccag
     1921 gcctacagaa ccagagtctc caaaaaatga ccgtgagggg cctcagtgac tcgaggaatt
     1981 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     2041 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     2101 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     2161 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     2221 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     2281 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     2341 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     2401 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     2461 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     2521 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2581 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag
     2641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2701 ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     2821 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     2881 ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt
     2941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg
     3061 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3181 ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc
     3241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4021 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4081 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4141 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4201 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4261 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4321 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4381 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4441 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4501 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4561 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4621 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4681 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4741 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4801 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4921 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4981 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
     5041 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     5101 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     5161 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     5221 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5281 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5341 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     5401 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     5461 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     5521 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg
     5581 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     5641 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     5701 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     5761 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     5821 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     5881 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     5941 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     6001 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     6061 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     6121 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     6181 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     6241 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     6301 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg
     6361 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     6421 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     6481 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     6541 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     6601 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     6661 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     6721 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg aa
//
