LOCUS       V008881                 7278 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008881
VERSION     V008881
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7278)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..2433
                     /gene="TNFAIP3"
                     /label="Tumor necrosis factor alpha-induced protein 3"
                     /note="Tumor necrosis factor alpha-induced protein 3 from
                     Homo sapiens. Accession#: P21580"
     misc_feature    2437..2480
                     /label="3' UTR of hA20"
                     /note="3' UTR of hA20"
     polyA_signal    2635..2690
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3051..3067)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3075..3091)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3099..3129)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3143..3164)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3222..3418
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    3424..3558
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3796..4384)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4558..5415)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5416..5520)
                     /label="AmpR promoter"
     enhancer        5551..5930
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5551..5928
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        5932..6209
                     /label="chicken beta-actin promoter"
     intron          6210..7227
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gcaatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg
      121 aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat cattcatcat
      181 tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt ttgtcctcag
      241 tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac cctggaaagc
      301 cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt
      361 gacggcaata gcctcatgca tgccacttct cagtacatgt ggggcgttca ggacacagac
      421 ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg caactttaaa
      481 ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg gctttgctat
      541 gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc cacagacaca
      601 cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat atttgtcctt
      661 tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag aagtttggaa
      721 tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct ccactggcct
      781 gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca ttttgtaccc
      841 ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt taacagagac
      901 cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa tgagatgaag
      961 gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg ctgggaccat
     1021 ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc
     1081 aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac
     1141 agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc cgtgccccag
     1201 ctttctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac
     1261 acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa caaactccca
     1321 aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg ggcctctcgg
     1381 ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg gcctcattcg
     1441 gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat gaagtgcagg
     1501 agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga acgttgccac
     1561 aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt ggatcccggg
     1621 aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg cagtacttgc
     1681 ttcaaaagga ctacagcaga ggcctcctcc agcctcagca ccagcctccc tccttcctgt
     1741 caccagcgtt ccaagtcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc
     1801 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg
     1861 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa
     1921 aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca ttttgctgct
     1981 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg
     2041 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt
     2101 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc
     2161 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc
     2221 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc
     2281 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag
     2341 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc
     2401 aacgaatgct ttcagttcaa gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct
     2461 gcaagaagtg gggcctcgag gtcgaggaat tcactcctca ggtgcaggct gcctatcaga
     2521 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     2581 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     2641 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     2701 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     2761 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     2821 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     2881 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     2941 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     3001 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     3061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3121 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3181 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
     3241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3421 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3541 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     3601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
     3721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5521 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
     5581 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     5641 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     5701 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5761 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     5821 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     5881 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     5941 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     6001 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
     6061 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
     6121 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     6181 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
     6241 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     6301 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     6361 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     6421 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     6481 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     6541 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     6601 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     6661 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     6721 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     6781 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     6841 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     6901 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
     6961 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
     7021 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
     7081 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
     7141 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
     7201 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     7261 catcattttg gcaaagaa
//
