LOCUS       V008883                 5912 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008883
VERSION     V008883
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label="E-tag"
                     /note="E-tag"
     CDS             64..1083
                     /gene="OPG199"
                     /label="Serine proteinase inhibitor 2"
                     /note="Serine proteinase inhibitor 2 from Cowpox virus
                     (strain Brighton Red). Accession#: P07385"
     polyA_signal    1269..1324
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1685..1701)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1709..1725)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1733..1763)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1777..1798)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1856..2052
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2058..2192
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2430..3018)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3192..4049)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4050..4154)
                     /label="AmpR promoter"
     enhancer        4185..4564
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4185..4562
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4566..4843
                     /label="chicken beta-actin promoter"
     intron          4844..5861
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gctgatatct tcagggaaat cgcatcttct atgaaaggag agaatgtatt catttctcca
      121 ccgtcaatct cgtcagtatt gacaatactg tattatggag ctaatggatc cactgctgaa
      181 cagctatcaa aatatgtaga aaaggaggcg gacaagaata aggatgatat ctcattcaag
      241 tccatgaata aagtatatgg gcgatattct gcagtgttta aagattcctt tttgagaaaa
      301 attggagata atttccaaac tgttgacttc actgattgtc gcactgtaga tgcgatcaac
      361 aagtgtgttg atatcttcac tgaggggaaa attaatccac tattggatga accattgtct
      421 ccagatacct gtctcctagc aattagtgcc gtatacttta aagcaaaatg gttgatgcca
      481 tttgaaaagg aatttaccag tgattatccc ttttacgtat ctccaacgga aatggtagat
      541 gtaagtatga tgtctatgta cggcgaggca tttaatcacg catctgtaaa agaatcattc
      601 ggcaactttt caatcataga actgccatat gttggagata ctagtatggt ggtaattctt
      661 ccagacaata ttgatggact agaatccata gaacaaaatc taacagatac aaattttaag
      721 aaatggtgtg actctatgga tgctatgttt atcgatgtgc acattcccaa gtttaaggta
      781 acaggctcgt ataatctggt ggatgcgcta gtaaagttgg gactgacaga ggtgttcggt
      841 tcaactggag attatagcaa tatgtgtaat tcagatgtga gtgtcgacgc tatgatccac
      901 aaaacgtata tagatgtcaa tgaagagtat acagaagcag ctgcagcaac ttgtgcgctg
      961 gtggcagact gtgcatcaac agttacaaat gagttctgtg cagatcatcc gttcatctat
     1021 gtgattaggc atgtcgatgg caaaattctt ttcgttggta gatattgctc tccaacaact
     1081 aattaacccg ggacattaaa cgcgtgggta ccggatccgg caattcactc ctcaggtgca
     1141 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     1201 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     1261 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     1321 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     1381 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     1441 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     1501 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     1561 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     1621 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     1681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1741 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat
     1801 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat
     1861 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1921 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1981 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2041 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2161 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc
     2221 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     2281 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2341 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2401 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2461 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2521 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2581 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2641 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2701 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2761 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2821 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2881 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2941 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3001 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3061 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3121 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3181 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3241 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3301 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3361 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3421 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3481 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3541 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3601 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3661 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3721 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3781 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3841 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3901 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3961 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     4021 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     4081 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4141 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac
     4201 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     4261 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4321 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4381 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4441 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4501 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4561 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     4621 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     4681 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa
     4741 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     4801 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg
     4861 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac
     4921 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
     4981 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg
     5041 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
     5101 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
     5161 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg
     5221 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     5281 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc
     5341 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt
     5401 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg
     5461 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
     5521 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     5581 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     5641 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     5701 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     5761 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     5821 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     5881 ttattgtgct gtctcatcat tttggcaaag aa
//
