LOCUS       40924_8181        5722 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-E-RIG-I-CARD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5722)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5722)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    16..54
                     /label=E-tag
                     /note="E-tag"
     CDS             64..915
                     /codon_start=1
                     /note="unnamed protein product; hDDX58 CARD"
                     /protein_id="SJL86630.1"
                     /translation="MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEK
                     NNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYR
                     LLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSD
                     KENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNL
                     SENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHH
                     LKK"
     polyA_signal    1079..1134
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1495..1511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1519..1535)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1543..1573)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1587..1608)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1666..1862
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    1868..2002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2240..2828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3002..3859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3860..3964)
                     /label=AmpR promoter
     enhancer        3995..4374
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3995..4372
                     /label=hCMV-IE enhancer
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4376..4653
                     /label=chicken beta-actin promoter
     intron          4654..5671
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
       61 gcaatgacca ccgagcagcg acgcagcctg caagccttcc aggattatat ccggaagacc
      121 ctggacccta cctacatcct gagctacatg gccccctggt ttagggagga agaggtgcag
      181 tatattcagg ctgagaaaaa caacaagggc ccaatggagg ctgccacact ttttctcaag
      241 ttcctgttgg agctccagga ggaaggctgg ttccgtggct ttttggatgc cctagaccat
      301 gcaggttatt ctggacttta tgaagccatt gaaagttggg atttcaaaaa aattgaaaag
      361 ttggaggagt atagattact tttaaaacgt ttacaaccag aatttaaaac cagaattatc
      421 ccaaccgata tcatttctga tctgtctgaa tgtttaatta atcaggaatg tgaagaaatt
      481 ctacagattt gctctactaa ggggatgatg gcaggtgcag agaaattggt ggaatgcctt
      541 ctcagatcag acaaggaaaa ctggcccaaa actttgaaac ttgctttgga gaaagaaagg
      601 aacaagttca gtgaactgtg gattgtagag aaaggtataa aagatgttga aacagaagat
      661 cttgaggata agatggaaac ttctgacata cagattttct accaagaaga tccagaatgc
      721 cagaatctta gtgagaattc atgtccacct tcagaagtgt ctgatacaaa cttgtacagc
      781 ccatttaaac caagaaatta ccaattagag cttgctttgc ctgctatgaa aggaaaaaac
      841 acaataatat gtgctcctac aggttgtgga aaaacctttg tttcactgct tatatgtgaa
      901 catcatctta aaaaatgact cgaggtcgag gaattcactc ctcaggtgca ggctgcctat
      961 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     1021 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     1081 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     1141 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     1201 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     1261 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt
     1321 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc
     1381 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc
     1441 cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata
     1501 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1561 cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1621 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     1681 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     1741 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     1801 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     1861 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1981 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     2041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2161 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2221 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2281 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2341 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2401 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2461 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2521 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2581 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2641 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2701 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2761 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2821 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2881 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2941 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3001 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3061 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3961 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa
     4021 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     4081 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     4141 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     4201 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     4261 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     4321 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
     4381 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
     4441 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc
     4501 cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg
     4561 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
     4621 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc
     4681 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
     4741 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
     4801 tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct
     4861 ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     4921 tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     4981 ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc
     5041 tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg
     5101 ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc
     5161 ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg
     5221 gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg
     5281 ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag
     5341 ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg
     5401 tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg
     5461 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
     5521 tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg
     5581 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     5641 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     5701 gtctcatcat tttggcaaag aa
//
