LOCUS       V008882                 7736 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008882
VERSION     V008882
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7736)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..49
                     /label="E-tag"
                     /note="E-tag"
     CDS             59..2926
                     /gene="CYLD"
                     /label="Ubiquitin carboxyl-terminal hydrolase CYLD"
                     /note="Ubiquitin carboxyl-terminal hydrolase CYLD from Homo
                     sapiens. Accession#: Q9NQC7"
     polyA_signal    3093..3148
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3509..3525)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3533..3549)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3557..3587)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3601..3622)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3680..3876
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    3882..4016
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4254..4842)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5016..5873)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5874..5978)
                     /label="AmpR promoter"
     enhancer        6009..6388
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      6009..6386
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        6390..6667
                     /label="chicken beta-actin promoter"
     intron          6668..7685
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcaat
       61 gagttcaggc ttatggagcc aagaaaaagt cacttcaccc tactgggaag agcggatttt
      121 ttacttgctt cttcaagaat gcagcgttac agacaaacaa acacaaaagc tccttaaagt
      181 accgaaggga agtataggac agtatattca agatcgttct gtggggcatt caaggattcc
      241 ttctgcaaaa ggcaagaaaa atcagattgg attaaaaatt ctagagcaac ctcatgcagt
      301 tctctttgtt gatgaaaagg atgttgtaga gataaatgaa aagttcacag agttactttt
      361 ggcaattacc aattgtgagg agaggttcag cctgtttaaa aacagaaaca gactaagtaa
      421 aggcctccaa atagacgtgg gctgtcctgt gaaagtacag ctgagatctg gggaagaaaa
      481 atttcctgga gttgtacgct tcagaggacc cctgttagca gagaggacag tctccggaat
      541 attctttgga gttgaattgc tggaagaagg tcgtggtcaa ggtttcactg acggggtgta
      601 ccaagggaaa cagctttttc agtgtgatga agattgtggc gtgtttgttg cattggacaa
      661 gctagaactc atagaagatg atgacactgc attggaaagt gattacgcag gtcctgggga
      721 cacaatgcag gtcgaacttc ctcctttgga aataaactcc agagtttctt tgaaggttgg
      781 agaaacaata gaatctggaa cagttatatt ctgtgatgtt ttgccaggaa aagaaagctt
      841 aggatatttt gttggtgtgg acatggataa ccctattggc aactgggatg gaagatttga
      901 tggagtgcag ctttgtagtt ttgcgtgtgt tgaaagtaca attctattgc acatcaatga
      961 tatcatccca gctttatcag agagtgtgac gcaggaaagg aggcctccca aacttgcctt
     1021 tatgtcaaga ggtgttgggg acaaaggttc atccagtcat aataaaccaa aggctacagg
     1081 atctacctca gaccctggaa atagaaacag atctgaatta ttttatacct taaatgggtc
     1141 ttctgttgac tcacaaccac aatccaaatc aaaaaataca tggtacattg atgaagttgc
     1201 agaagaccct gcaaaatctc ttacagagat atctacagac tttgaccgtt cttcaccacc
     1261 actccagcct cctcctgtga actcactgac caccgagaac agattccact ctttaccatt
     1321 cagtctcacc aagatgccca ataccaatgg aagtattggc cacagtccac tttctctgtc
     1381 agcccagtct gtaatggaag agctaaacac tgcacccgtc caagagagtc cacccttggc
     1441 catgcctcct gggaactcac atggtctaga agtgggctca ttggctgaag ttaaggagaa
     1501 ccctcctttc tatggggtaa tccgttggat cggtcagcca ccaggactga atgaagtgct
     1561 cgctggactg gaactggaag atgagtgtgc aggctgtacg gatggaacct tcagaggcac
     1621 tcggtatttc acctgtgccc tgaagaaggc gctgtttgtg aaactgaaga gctgcaggcc
     1681 tgactctagg tttgcatcat tgcagccggt ttccaatcag attgagcgct gtaactcttt
     1741 agcatttgga ggctacttaa gtgaagtagt agaagaaaat actccaccaa aaatggaaaa
     1801 agaaggcttg gagataatga ttgggaagaa gaaaggcatc cagggtcatt acaattcttg
     1861 ttacttagac tcaaccttat tctgcttatt tgcttttagt tctgttctgg acactgtgtt
     1921 acttagaccc aaagaaaaga acgatgtaga atattatagt gaaacccaag agctactgag
     1981 gacagaaatt gttaatcctc tgagaatata tggatatgtg tgtgccacaa aaattatgaa
     2041 actgaggaaa atacttgaaa aggtggaggc tgcatcagga tttacctctg aagaaaaaga
     2101 tcctgaggaa ttcttgaata ttctgtttca tcatatttta agggtagaac ctttgctaaa
     2161 aataagatca gcaggtcaaa aggtacaaga ttgttacttc tatcaaattt ttatggaaaa
     2221 aaatgagaaa gttggcgttc ccacaattca gcagttgtta gaatggtctt ttatcaacag
     2281 taacctgaaa tttgcagagg caccatcatg tctgattatt cagatgcctc gatttggaaa
     2341 agactttaaa ctatttaaaa aaatttttcc ttctctggaa ttaaatataa cagatttact
     2401 tgaagacact cccagacagt gccggatatg tggagggctt gcaatgtatg agtgtagaga
     2461 atgctacgac gatccggaca tctcagctgg aaaaatcaag cagttttgta aaacctgcaa
     2521 cactcaagtc caccttcatc cgaagaggct gaatcataaa tataacccag tgtcacttcc
     2581 caaagactta cccgactggg actggagaca cggctgcatc ccttgccaga atatggagtt
     2641 atttgctgtt ctctgcatag aaacaagcca ctatgttgct tttgtgaagt atgggaagga
     2701 cgattctgcc tggctcttct ttgacagcat ggccgatcgg gatggtggtc agaatggctt
     2761 caacattcct caagtcaccc catgcccaga agtaggagag tacttgaaga tgtctctgga
     2821 agacctgcat tccttggact ccaggagaat ccaaggctgt gcacgaagac tgctttgtga
     2881 tgcatatatg tgcatgtacc agagtccaac aatgagtttg tacaaataag acctcgaggt
     2941 cgaggaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     3001 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     3061 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     3121 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     3181 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     3241 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     3301 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     3361 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     3421 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     3481 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3541 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta
     3601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3661 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3721 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3781 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3841 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     3901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     4021 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4081 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4141 ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga
     4201 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4441 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4681 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6001 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     6061 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     6121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     6181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     6241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     6301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     6361 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     6421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     6481 tgcagcgatg ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga
     6541 ggggcggggc ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     6601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     6661 gcgggcggga gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg
     6721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     6781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     6841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     6901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     6961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     7021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     7081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     7141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     7201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     7261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga
     7321 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     7381 agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc
     7441 gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg
     7501 gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg
     7561 gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct
     7621 ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc
     7681 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaa
//
