LOCUS       V008884                 5363 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008884
VERSION     V008884
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5363)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5363)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    19..57
                     /label="E-tag"
                     /note="E-tag"
     CDS             67..651
                     /gene="PYCARD"
                     /label="Apoptosis-associated speck-like protein containing
                     a CARD"
                     /note="Apoptosis-associated speck-like protein containing a
                     CARD from Homo sapiens. Accession#: Q9ULZ3"
     polyA_signal    723..778
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(1139..1155)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1163..1179)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1187..1217)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1231..1252)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1310..1506
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    1512..1646
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1884..2472)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2646..3503)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3504..3608)
                     /label="AmpR promoter"
     enhancer        3639..4018
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      3639..4016
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4020..4297
                     /label="chicken beta-actin promoter"
     intron          4298..5315
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 gaattgaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg
       61 gccgctatgg ggcgcgcgcg cgacgccatc ctggatgcgc tggagaacct gaccgccgag
      121 gagctcaaga agttcaagct gaagctgctg tcggtgccgc tgcgcgaggg ctacgggcgc
      181 atcccgcggg gcgcgctgct gtccatggac gccttggacc tcaccgacaa gctggtcagc
      241 ttctacctgg agacctacgg cgccgagctc accgctaacg tgctgcgcga catgggcctg
      301 caggagatgg ccgggcagct gcaggcggcc acgcaccagg gctctggagc cgcgccagct
      361 gggatccagg cccctcctca gtcggcagcc aagccaggcc tgcactttat agaccagcac
      421 cgggctgcgc ttatcgcgag ggtcacaaac gttgagtggc tgctggatgc tctgtacggg
      481 aaggtcctga cggatgagca gtaccaggca gtgcgggccg agcccaccaa cccaagcaag
      541 atgcggaagc tcttcagttt cacaccagcc tggaactgga cctgcaagga cttgctcctc
      601 caggccctaa gggagtccca gtcctacctg gtggaggacc tggagcggag ctgaagatct
      661 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
      721 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
      781 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
      841 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
      901 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
      961 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1021 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1081 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     1141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1201 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
     1261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     1321 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1381 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1441 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1501 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1621 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     1681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1801 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3601 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
     3661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3721 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3781 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3841 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3901 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3961 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4021 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     4081 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
     4141 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
     4201 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     4261 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
     4321 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     4381 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     4441 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     4501 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     4561 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     4621 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     4681 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     4741 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     4801 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     4861 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     4921 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     4981 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     5041 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     5101 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     5161 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     5221 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     5281 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
     5341 tgctgtctca tcattttggc aaa
//
