LOCUS       V008886                 6261 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008886
VERSION     V008886
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6261)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..296
                     /label="5' UTR of cowpox virus crmA"
                     /note="5' UTR of cowpox virus crmA"
     CDS             297..1319
                     /gene="OPG199"
                     /label="Serine proteinase inhibitor 2"
                     /note="Serine proteinase inhibitor 2 from Cowpox virus
                     (strain Brighton Red). Accession#: P07385"
     misc_feature    1323..1468
                     /label="3' UTR of cowpox virus crmA"
                     /note="3' UTR of cowpox virus crmA"
     polyA_signal    1618..1673
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(2034..2050)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2058..2074)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2082..2112)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2126..2147)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2205..2401
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    2407..2541
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2779..3367)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3541..4398)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4399..4503)
                     /label="AmpR promoter"
     enhancer        4534..4913
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4534..4911
                     /label="hCMV-IE enhancer"
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     promoter        4915..5192
                     /label="chicken beta-actin promoter"
     intron          5193..6210
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttcccatgga agaacgaaag tagtataaaa gtaataaaac aaaaaaaaga atataaaaaa
       61 tttatagcca ctttctttga ggactgtttt cctgaaggaa atgaacctct ggaattagtt
      121 agatatatag aattagtata cacgctagat tattctcaaa ctcctaatta tgacagacta
      181 cgtagactgt ttatacaaga ttgaaaatat atttcttttt attgagtggt ggtagttacg
      241 gatatctaat attaatatta gactatctct atcgtcacac aacaaaatcg attgccatgg
      301 atatcttcag ggaaatcgca tcttctatga aaggagagaa tgtattcatt tctccaccgt
      361 caatctcgtc agtattgaca atactgtatt atggagctaa tggatccact gctgaacagc
      421 tatcaaaata tgtagaaaag gaggcggaca agaataagga tgatatctca ttcaagtcca
      481 tgaataaagt atatgggcga tattctgcag tgtttaaaga ttcctttttg agaaaaattg
      541 gagataattt ccaaactgtt gacttcactg attgtcgcac tgtagatgcg atcaacaagt
      601 gtgttgatat cttcactgag gggaaaatta atccactatt ggatgaacca ttgtctccag
      661 atacctgtct cctagcaatt agtgccgtat actttaaagc aaaatggttg atgccatttg
      721 aaaaggaatt taccagtgat tatccctttt acgtatctcc aacggaaatg gtagatgtaa
      781 gtatgatgtc tatgtacggc gaggcattta atcacgcatc tgtaaaagaa tcattcggca
      841 acttttcaat catagaactg ccatatgttg gagatactag tatggtggta attcttccag
      901 acaatattga tggactagaa tccatagaac aaaatctaac agatacaaat tttaagaaat
      961 ggtgtgactc tatggatgct atgtttatcg atgtgcacat tcccaagttt aaggtaacag
     1021 gctcgtataa tctggtggat gcgctagtaa agttgggact gacagaggtg ttcggttcaa
     1081 ctggagatta tagcaatatg tgtaattcag atgtgagtgt cgacgctatg atccacaaaa
     1141 cgtatataga tgtcaatgaa gagtatcgcg aagcggctgc agcaacttgt gcgctggtgg
     1201 cagactgtgc atcaacagtt acaaatgagt tctgtgcaga tcatccgttc atctatgtga
     1261 ttaggcatgt cgatggcaaa attcttttcg ttggtagata ttgctctcca acaactaatt
     1321 aaatcacatt cttaatatta gaatattaga atattatata gttaagattt ttactaattg
     1381 gttaaccatt tttttaaaaa aatagaaaaa aaacatgtta tattagcgag ggtcgttatt
     1441 cttccaattg caattggtaa gatgacgggg aattcactcc tcaggtgcag gctgcctatc
     1501 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     1561 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     1621 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     1681 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     1741 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     1801 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     1861 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     1921 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     1981 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     2041 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2101 ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     2221 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     2281 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     2341 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     2401 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2521 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2581 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2641 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3181 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3301 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4501 gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat
     4561 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     4621 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     4681 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     4741 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     4801 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     4861 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
     4921 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     4981 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc
     5041 ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg
     5101 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     5161 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg
     5221 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     5281 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     5341 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
     5401 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
     5461 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     5521 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
     5581 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
     5641 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
     5701 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
     5761 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     5821 gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
     5881 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
     5941 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
     6001 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
     6061 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
     6121 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
     6181 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
     6241 tctcatcatt ttggcaaaga a
//
